Şadiye KUM, Bülent BOZDOĞAN, İlknur DABANOĞLU

Testosteron Takviyesinin Yaşlı Farelerin Kalbinde Kapillar Morfometri ve VEGF Expresyon Seviyesine Etkisi

Bu çalışmanın amacı yaşlı farelerin kalbinde testosteron hormon takviyesinin anjiyogenik etkisini moleküler ve histolojik olarak tespit etmektir. Toplam 30 fareden 3 çalışma grubu oluşturulmuştur. Bunlar; yalancı operasyon yapılan (kontrol) grup, gonadektomi yapılan (G) grup ve hem gonadektomi hem de testosteron takviyesi yapılan (GTS) gruptur. Kalp dokusunda kapillar sayısı ve geniş kapillar damarların iç çapı ışık mikroskobunda ölçülmüştür. Kalp dokusundaki VEGF (vasküler endoteliyal büyüme faktörü) mRNA seviyesi RT-PCR ile ölçülmüştür. Dişi farelerde; kontrol grubuna göre G ve GTS (P

The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice

The aim of this study was to determine the effct of testosterone hormone supplementation on the capillary morphometry and VEGFexpression level of the heart of old mice using histological and molecular techniques. A total of 30 old mice were enrolled in 3 groups: shamoperation group (Control), gonadectomy group (G) and gonadectomy and testosterone supplementation group (GTS). The capillary numberand the inner diameter of larger capillary vessels in the heart were measured by light microscope in terms of angiogenic processes. The levelsof angiogenic factor, VEGF (vascular endothelial growth factor), mRNA in the heart tissue were determined by RT-PCR. The capillary densitiesdecreased in female mice, in which G and GTS (P

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