Ayona SILVA-FLETCHER, Puntia SIENGDEE, Korakot NGANVONGPANIT, Dheerawan BOONYAWAN, Chatchote THITARAM, Sarisa KLINHOM

Termal Olmayan Plazma Enerjisi Uygulanan Besiyeri Ortamının Asya Fillerinin (Elephas maximus) Fibroblast Hücrelerinin Büyüme ve Canlılığı Üzerine In-vitro Etkisi

Termal olmayan plazma (NTP), yara iyileşmesinin hızlandırılması, enfektif mikroorganizmaların eliminasyonu ve kanser tedavisi gibi çeşitli tıbbi uygulamalar için geliştirilmektedir. Bu çalışmada NTP’ye maruz burakılan besiyeri ortamının Asya fillerinden elde edilen deri fibroblastlarının in vitro proliferasyonu üzerindeki etkisi araştırıldı. Bu amaçla Dulbecco’nun Modifiye Eagle’s Medium’u (DMEM) besiyeri olarak kullanıldı ve üç farklı yoğunlukta NTP’ye maruz bırakıldı. NTP reaktif türleri Nitrit (NO2-) hücrelere eklenmeden önce uygulandığı besiyerinde ölçüldü. Fibroblastlar, 24 saat boyunca, %10 Fetal Sığır Serumu (FBS) ve %1 antibiyotik/antimikotik içeren NTP ile muamele edilmiş besiyerinde inkübe edildi. İnkübasyonun başlamasından 24, 48 ve 72 saat sonra hücre proliferasyonu, hücre sayısı ve canlılık oranı akış sitometrisi kullanılarak analiz edildi. NTP ile muamele edilmiş besiyerinde inkübe edilen fibroblastların proliferasyon hızı, kontrol grubundan anlamlı olarak daha yüksek bulundu (P

Effect of Culture Medium Treated with Non-thermal Plasma Energy on the Growth and Viability In-vitro of Fibroblast Cells from Asian Elephants (Elephas maximus)

Non-thermal plasma (NTP) is being developed for a wide-range of medical applications such as improvement of wound healing, elimination of infective microorganisms, and treatment of cancer. This study investigated the effct of culture medium exposed to NTP on the proliferation in-vitro of skin fibroblasts from Asian elephants. Dulbecco’s Modified Eagle’s Medium (DMEM) was used as culture medium and was exposed to NTP with three diffrent intensities. The NTP reactive species Nitrite (NO2-) was measured in the treated medium before addition to cells. Fibroblasts were incubated for 24 h with NTP-treated complete medium supplemented with 10% Fetal Bovine Serum (FBS) and 1% antibiotic/antimycotic. Cell proliferation, the number of cells and viability rate were analysed using flw cytometry 24, 48 and 72 h after the start of the incubation. The proliferation rate of fibroblasts incubated with NTP treated medium was significantly higher (P

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