ABDURRAHMAN KÖSEMAN, YUSUF ÖZŞENSOY, METİN ERDOĞAN, Cevdet YARALI, Burhan TOPRAK, Kemal ZENGİN, İbrahim ŞEKER

Investigation of Genetic Structures of Coloured Horses by mtDNA D-loop Sequence Analysis in Turkey

The aim of this study was to determine the genetic structure of Coloured horses in Turkey by analysis of the D-loop sequence of mitochondrial DNA (mtDNA). A total of 28 Coloured horses were examined. DNA was extracted from blood samples using DNA isolation kit; 519 bp long mtDNA D-loop region was amplified by PCR and sequenced by capillary electrophoresis system. Population parameters and phylogenetic trees were drawn by using MEGA4 software package. It was also compared with the DNA sequences of horse populations from diffrent countries. In this study, 42 diffrent polymorphism regions and 10 diffrent haplogroups were detected. Additionaly, Tajima D value was found to be -0.31 and population expansion was determined. It was determined that the base diffrences among the horses ranged between 0.000 and 0.032. It was detected that the horses formed diffrent clusters from each other and they were intertwined with the populations of diffrent countries. Moreover, it was also observed that some horses formed their own populations at diffrent points from the other countries’ horses. As a result, it was observed that the genetic structures of horses which used in population were diffrent from each other and they originated from diffrent mothers. According to the results obtained, it is considered that Coloured horses can be a native horse breed of Turkey.

Türkiye’deki Alaca Atların mtDNA D-Loop Dizi Analizi İle Genetik Yapılarının Araştırılması

Bu çalışma, Türkiye’deki Alaca atların mtDNA D-Loop dizi analizi ile genetik yapılarının belirlenmesi amacıyla yapılmıştır. Araştırmada, toplam 28 Alaca at incelenmiştir. Alınan kan örneklerinden DNA izolasyon kiti kullanılarak DNA elde edilmiş ve 519 bp uzunluğundaki mtDNA D-loop bölgesi PZR ile çoğaltılmış ve kapiller elektroforez sisteminde dizileme işlemi yapılmıştır. MEGA4 paket programı kullanılarak populasyon parametreleri ve filogenetik ağaçları çizilmiştir. Ayrıca farklı ülke at populasyonlarına ait DNA dizileri ile de karşılaştırılması yapılmıştır. Araştırmada, 42 farklı polimorfizm bölgesi ve 10 farklı haplogrup elde edilmiştir. Ayrıca Tajima D değeri -0.31 elde edilmiş ve populasyon genişlemesi olduğu belirlenmiştir. Örnekler arasındaki baz faklılıklarının 0.000 ile 0.032 arasında değiştiği belirlenmiştir. Çizilen filogenetik ağaçlar sonucunda örneklerin birbirlerinden farklı kümeler oluşturduğu ve farklı ülke populasyonları ile de iç içe girdiği belirlenmiştir. Ayrıca, bazı örneklerin farklı ülke atlarından tamamen ayrı noktalarda kendi populasyonunu oluşturduğu da gözlenmiştir. Sonuç olarak çalışılan populasyondaki örneklerin genetik yapılarının birbirinden farklı olduğu ve farklı analardan köken aldıkları görülmüştür. Elde edilen sonuçlara göre; Alaca atların Türkiye’ye ait yerli bir ırk olabileceği düşünülmektedir.

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