ERDOĞAN UZLU, MEHMET ÇİTİL, HİDAYET METİN ERDOĞAN, ONUR ATAKİŞİ, KÜRŞAD YAPAR

Tavşanlarda farklı nonsteroidal antiinflamatorik ilaçların serbest triiyodotronin (fT3), serbest troksin (fT4) ve troid stimulan hormon (TSH) konsantrasyonlarına etkileri.

Bu çalışmada hekimliğimizde sıklıkla kullanım alanı bulan Flunixin meglimune, Carprofen ve Meloxicam gibi nonsteroidal antiinflamatorik ilaçların (NSAİD) beş günlük kullanım süresince serbest triiyodotronin (fT3), serbest troksin (fT4) ve troid stimulan hormon (TSH) konsantrasyonları üzerine olan etkilerinin belirlenmesi amaçlandı. Bu amaçla Yeni Zelanda ırkı, farklı cinsiyet ile benzer yaş ve ağırlıkta toplam 32 tavşan kullanıldı. Tavşanlar uygulama çeşitliğine göre 8’erli 4 gruba ayrıldı. Kontrol grubundaki tavşanlara serum fizyolojik, ikinci grup (FLU) tavşanlara 1.1 mg/kg dozda flunixin meglumine, üçüncü grup (CAR) tavşanlara 2.2 mg/kg dozda carprofen ve dördüncü grup (MEL) tavşanlara 0.2 mg/kg dozda meloxicam 5 gün süreyle günde bir kez İM uygulandı. Hormon konsantrasyonlarının tespiti amacı ile İlaç uygulamasından 4 saat sonra vena auricularis’ten usulüne uygun olarak antikoagulantsız ependorf tüplere alınan kan örnekleri 3000 rpm’de 10 dakika santrifüj edilerek serum elde edildi. Serbest T3, T4 ve TSH ölçümleri ticari ELISA kitler kullanılarak spektrofotometrik olarak yapıldı. Çalışma sonucunda NSAİD uygulanan her üç grupta ve beş günlük deneme süresince fT3 ve fT4 konsantrasyonlarının arttığı (p

Anahtar Kelimeler:

sıçan, tiroksin, tiroid hormonları, triiyodotironin, tirotropin, ilaçların etkileri, antienflamatuar ajanlar

Effects of different nonsteroid antiinflammatory drugs on free triiodothyronin (fT3), free thyroxin (fT4) and thyroid stimulating hormone concentration in rabbits

This study was aimed to investigate the effects of commonly prescribed NSAIDs, flunixin meglumine, carprofen and meloxicam, on the concentrations of free triiodothyronin (fT3), free thyroxin (fT4) and thyroid stimulating hormone in healthy rabbits. For this purpose, 32 New Zealand rabbits of similar age and weight and different sex were divided into four equal groups of eight. Rabbits in control group (n=8) received serum physiologic, animals in group FLU (n=8) were given flunixin meglumine at dose of 1.1 mg/kg, group CAR (n=8) received 2.2 mg/kg carprofen and group MEL (n=8) was given 0.2 mg/kg meloxicam intramuscularly (IM) for 5 days. Blood samples were collected 4 hours after each injection from vena auricularis into plain vials to determine hormone concentrations. Following separation of serum by centrifugation at 3000 rpm for 10 minutes, commercially available ELISA kits for free T3, freeT3 and TSH were used for determination. Results shown that fT3, and fT4 concentrations increased throughout the study period of 5 days within 3 treatment groups (p<0.001) and these increases were also significant when compared to control group (p<0.05, p<0.01 and p<0.001) while TSH concentrations decreased in treatment groups (p<0.001) during the study period and it was also lower than the control group (p<0.001). In conclusion, use of NSAIDs can alter thyroid hormone concentrations and thus these alterations should be considered in diagnosis and therapy in order to avoid misleading.

Keywords:

rats, thyroxine, thyroid hormones, triiodothyronine, thyrotropin, drug effects, antiinflammatory agents,

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