KÜRŞAD YAPAR, EMİNE ATAKİŞİ, ERDOĞAN UZLU, ONUR ATAKİŞİ, MEHMET ÇİTİL, Metehan UZUN, HİDAYET METİN ERDOĞAN

Farklı dozlardaki metamizol sodyum’un farelerde serum enzim aktiviteleri ile karaciğer ve böbrek dokularındaki oksidant seviyeleri üzerine etkileri

Metamizol sodyum (MS), veteriner sahada yaygın olarak kullanılan pirazolon türevi narkotik olmayan ağrı kesici ilaçlardandır. Bu çalışma, farelere farklı dozda metamizol sodyum uygulamalarının serum üre, kreatinin, alanin aminotransferaz (ALT), aspartat aminotransferaz (AST) ve alkalen fosfataz (ALP) enzim aktiviteleri ile karaciğer ve böbrek doku malondialdehit (MDA) ve indirgenmiş glutatyon (GSH) düzeyleri üzerine olan etkilerini araştırmak amacıyla yapıldı. Çalışmada toplam 32 adet Swiss Albino fare kullanıldı ve hayvanlar 4 eşit gruba (n=8) ayrıldı. Bir grup kontrol olarak ayrılırken, diğer üç gruptaki farelere 7 gün süre ile her gün 20 (Grup I), 50 (Grup II) ve 100 mg/kg (Grup III) olmak üzere normal, orta ve yüksek dozlarda MS kas içi uygulandı. Yedi günlük uygulamadan sonra farelerden kan ve doku örnekleri alındı. Serum örneklerinden üre ve kreatinin düzeyleri, ALT, AST ve ALP enzim aktiviteleri ile karaciğer ve böbrek doku süpernatantlarında MDA ve GSH analizleri yapıldı. Çalışmada, yüksek doz (100 mg/kg) uygulanan deney grubu serum üre ve kreatinin düzeyleri ile ALT, AST ve ALP enzim aktiviteleri, karaciğer ve böbrek dokusunda MDA miktarı, kontrol ve diğer iki gruba göre yüksek (p

Anahtar Kelimeler:

enzim aktivitesi, fareler, karaciğer, böbrekler, toksisite, ağrı kesiciler

The effect of different doses of metamisole sodium on serum enzyme activities and tissue oxidant levels in liver and kidney in mice

Metamisole sodium (MS), a pyrosilone derivate, is widely used non-opioid analgesic in veterinary medicine. This study evaluated the effect of different doses of metamisole sodium on serum urea, creatin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phophatase (ALP) activities and tissue malondialdehyde (MDA) and reduced glutathione (GSH) level in liver and kidney in mice. The study involved 32 Swiss Albino mice. Animals were divided into four equal groups. One group was left control the other three groups received 20 (Group I), 50 (Group II) and 100 mg/kg (Group III) MS intramuscularly for 7 days. After a seven-day injection blood and tissue samples were collected from mice. Serum samples were examined for the determination of BUN, creatin, ALT, AST and ALP while tissue samples were evaluated for MDA and GSH levels. The high dose group (100mg/kg) had higher concentrations of serum BUN, creatin, ALT, AST and tissue MDA but low concentration of GSH (p<0.05) when compared to other groups. In conclusion, short term high dose (100 mg/kg) of MS resulted in liver and kidney degeneration.

Keywords:

enzyme activity, mice, liver, kidneys, toxicity, analgesics,

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