AHMET CEYLAN, İlker SERİN, HASAN AKŞİT, Kamil SEYREK, CENGİZ GÖKBULUT

Döl tutmayan veanöstruslu süt ineklerinde vitamin A, E, Beta-karoten, kolesterol ve trigliserid düzeylerinin araştırılması.

Vitamin A, E ve β-karoten süt ineklerinde normal reprodüktif faaliyetlerin yürütülmesi için oldukca önemlidir. Bu çalışmanın amacı, döl tutmayan ve anöstruslu ineklerde vitamin A, E ve beta-karoten ile kolesterol ve trigliserid gibi bazı biyokimyasal parametrelerin serum düzeylerini tespit etmek ve reprodüktif problemlerin ortaya çıkmasındaki olası rollerini araştırmaktır. Çalışma, yaşları 3–8 arasında değişen, toplam 64 baş Holştein ırkı inek üzerinde Mart-Kasım aylarında gerçekleştirildi. Deneme grubunu 44 adet reprodüktif problemli inek, kontrol grubunu ise 20 adet sağlıklı inek oluşturdu. Deneme grubuda kendi içinde döl tutmayan (n=26) ve anöstrus grubu (n=18) olarak ikiye ayrıldı. İneklerin kan serum vitamin A, beta-karoten, kolesterol ve trigliserid düzeyleri spektrofotometrik yöntemle ölçüldü. Serum vitamin E düzeyi ise yüksek performanslı sıvı kromatografisi (HPLC) kullanılarak analiz edildi. Döl tutmayan ve anöstruslu grupların vitamin E düzeyleri kontrol grubuna göre istatistiksel olarak daha düşük bulundu (P

Anahtar Kelimeler:

anöstrüs, beta-karoten, kolesterol, inek, kısırlık, döl tutmayan, retinol, triyosilgliserol, E vitamini

Investigation of vitamins A, E, beta-carotene, cholesterol and triglyceride concentrations in dairy cows with repeat breeder and anestrus

Vitamins A, E and beta-carotene are essential to maintaining normal reproduction in dairy cattle. The objective of this study were to determined serum concentrations of vitamins A, E, and beta-carotene as well as some biochemical parameters as kolosterol and trigliseride in dairy cows with repeat breeder and anestrus and their role on the etiology of these reproductive problems were investigated. The Study has been carried out between the months of March and November and total 64 Holstein cows. Fourty-four cows with reproductive problems and twenty controls were used in the study. Cows with reproductive problems were divided into two groups as repeat breeder (n=26) and anestrus group (n=18). Vitamin A, beta-carotene, cholesterol and triglyceride concentrations were determined by spectrohpotometrically. Vitamin E level in serum were analysed using validated a method with high performance liquid chromatography (HPLC). Vitamin E levels in the repeat breeder and anestrus groups were significantly lower than that in the control group (P<0.05). Although the serum levels of vitamins A and beta-carotene were lower in the cows with repeat breeder and anestrus than in the control group cows, the difference was not statistically significant. The triglyceride concentration in cows with repeat breeder was significantly higher (P<0.05) than those in the control group. It was also noted that the level of cholesterol was no statistical difference between these three groups. In conclusion, the low concentrations of vitamins E, A and beta-carotene analyzed may have some effects on reproductive problems as repeat breeder and anestrus.

Keywords:

anoestrus, beta-carotene, cholesterol, cows, infertility, repeat breeders, retinol, triacylglycerols, vitamin E,

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