Sunset yellow FCF' nin tavuk embriyosu deri ve barsak mast hücrelerinin degranülasyonu üzerindeki etkileri

Bu çalışmada, hazır gıdalarda yapay renklendirici olarak kullanılan sunset yellowun (E110) tavuk embriyosu dermal ve mukozal mast hücrelerindeki degranülasyon etkisi histolojik yönden incelenmiştir. Sunset yellow (2.5 mg/kg) inkübasyonun 15. gününde vitellusa enjekte edilmiştir. Enjeksiyonundan 6, 12 ve 24 saat sonra dermal ve mukozal mast hücrelerindeki degranülasyon etkisi incelenmiştir. Dermal mast hücrelerinde (deri) en fazla kısmi degranülasyon sunset yellow enjeksiyondan 12 saat, mukozal mast hücrelerinde (barsak) 6 saat sonra, en fazla ileri düzeyde degranülasyon ise deride enjeksiyondan 24 saat, barsakta 12 saat sonra gözlenmiştir. Barsaktaki mukozal mast hücrelerinde ileri degranülasyonun enjeksiyondan 24 saat sonra da devam ettiği belirlenmiştir. Her iki dokuda kısmi degranüle mast hücresi granüllerinin gevşek ve kaba granüller oluşturduğu, ileri degranülasyon gösteren mast hücrelerinin ise daha az granül içerdiklerinden dolayı daha aydınlık sitoplazmaya sahip oldukları görülmüştür.

Degranulation effects of sunset yellow-FCF on dermal and intestinal mast cells of chicken embryo

In this study, the degranulation effect of sunset yellow (E110) which is used as artificial coloring agent in ready food was histologically examined on dermal and mucosal mast cells of chicken embryo. Sunset yellow (2.5 mg/kg) was injected into vitellus of eggs on the 15th day of incubation. Degranulation of dermal and mucosal mast cells were evaluated 6, 12, 24 h after injection. The most of partial degranulated in dermal mast cells (skin) was observed after 12 h from injection of sunset yellow, and after 6 h in mucosal mast cells (intestine). High level degranulation in skin was showed after 24 h from injection, and after 12 h in intestine. Also, it was seen that high level degranulation in the mucosal mast cells of intestine were continued after 24 h from injection. In both types of tissues, it was found that partially degranulated mast cells formed loose and coarse granules while mast cells showing high level of degranulation have brighter cytoplasm since they contain less granuless.

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