DİLEK ÖZTÜRK, Sırrı AVKİ, Hülya TÜRÜTOĞLU, KÜRŞAD YİĞİTARSLAN, Sonay SAĞNAK

Methicillin resistance Among coagulase-positive Staphylococci isolated from dogs with otitis externa, skin wounds and pyoderma

Bu çalışmada, otitis eksterna, deri yarası ve pyodermalı 96 köpekten elde edilen 54 koagülaz-pozitif stafilokok (33 Staphylococcus aureus ve 21Staphylococcus intermedius) izolatı metisilin (oksasilin) direnci yönünden incelendi. Otitis eksterna ve deri enfeksiyonu tespit edilen köpeklerden en fazla izole edilen koagülaz-pozitif stafilokokun, S. aureus olduğu belirlendi. Koagülaz-pozitif stafilokoklar arasında disk diffüzyon testi ile metisiline fenotipik olarak dirençli bulunan dört S. aureus ve bir S. intermedius izolatının mecA geni taşımadıkları ve amoksisilin/klavulanik aside duyarlı oldukları saptandı. Fenotipik metisilin direnci belirlenen beş koagülaz-pozitif stafilokok izolatının ikisi (%2.2) otitis eksterna, ikisi (%15.4) pyoderma ve biri de (%3.2) deri yarasına sahip köpeklerden elde edildi. Çalışmadan elde edilen sonuçlar, otitis eksterna ve deri enfeksiyonlarına sahip köpeklerde en yaygın izole edilen etkenlerin metisiline duyarlı veya dirençli S. aureus ile S. intermedius olduğunu ve metisilin direncinin hızlı ve doğru bir şekilde teşhis edilmesinin, bu köpeklerin tedavisi için büyük öneme sahip olduğunu göstermektedir.

Anahtar Kelimeler:

hastalık kontrolü, Staphylococcus aureus, Staphylococcus intermedius, amoksilin, klavulanik asit, teşhis, yara, piyoderma, fenotip, oksasilin, dış kulak iltihabı, metisilin, genler, ilaç tedavisi, ilaç direnci, köpek

Otitis eksterna, deri yarası ve pyodermalı köpeklerden izole edilen koagülaz pozitif stafilokokların metisilin dirençliliği

In this study, 54 coagulase-positive staphylococci (33 Staphylococcus aureus and 21 Staphylococcus intermedius) isolated from 96 dogs with otitis externa, skin wounds and pyoderma were investigated for the methicillin (oxacillin) resistance. S. aureus was detected as the predominant coagulase-positive staphylococci in dogs affected by otitis externa and skin infections. Among coagulase-positive staphylococci, four S. aureus and one S. intermedius isolates, which did not contain mecA gene were found phenotypically resistant to methicillin, but were susceptible to amoxicillin/clavulanic acid by the disk diffusion test. Five phenotypic methicillin-resistant coagulase-positive staphylococci were isolated from two (2.2%) dogs with otitis externa, two (15.4%) dogs with pyoderma and one (3.2%) dog with skin wound. The results of this study shows that methicillin-susceptible or -resistant S. aureus and S. intermedius are the predominate organisms in dogs with otitis externa and skin infections, and the rapid and correct diagnosis of methicillin-resistance is of great importance for the treatment of dogs.

Keywords:

disease control, Staphylococcus aureus, Staphylococcus intermedius, amoxicillin, clavulanic acid, diagnosis, wounds, pyoderma, phenotypes, oxacillin, otitis externa, methicillin, genes, drug therapy, drug resistance, dogs,

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