Saad Hashim AL-HUSSEINY, Qassim Haleem KSHASH, Asaad JASSIM

Sero-detection of Foot and Mouth Disease Virus Serotypes A and O in One-humped Camels (Camelus dromedarius) in the Middle of Iraq

Foot and mouth disease (FMD) is a severe trans-boundary animal disease caused by a foot and mouth disease virus (FMDV) that spreads through Asia and Arabic countries. The current study aimed to investigate the FMDV antibodies in Iraqi dromedary’s camels (Camelus dromedaries). A total of 520 serum samples were collected from clinically healthy camels (265 females and 255males) from different areas in the three Provinces of Iraq (Al-Najaf, Al-Muthanna, and Al-Qadisiyah) from February to July 2019 and divided into three groups based on the age of the camels. All sera samples were screened for antibodies against the non-structural protein (NSP) of FMDV using ELISA and further the NSP positive serum samples were screened for antibodies against structural proteins of FMDV serotype O A and C by liquid phase blocking ELISA (LPB-ELISA).The Result indicated that 10% of the sera were positive for NSP antibodies and FMDV serotype A found to be predominant. It was also observed that NSP positive was more in samples collected from female camels (11.30%) than male camels (8.60%), NSP FMDV antibodies were detected in camels of all ages. In summary, our study showed, for the first time in Iraq, that camels are more susceptible to the A and O FMD serotypes.

Irak’ın Orta Bölgesinde Tek Hörgüçlü Develerde (Camelus dromedarius) Şap Hastalığı Virüsü Serotipleri A ve O’nun Sero-Tespiti

Şap hastalığı (FMD), Asya ve Arap ülkelerine yayılan ayak ve ağız hastalığı virüsünün (FMDV) neden olduğu ciddi bir sınır ötesi hayvan hastalığıdır. Bu çalışmada Irak tek hörgüçlü develerinde (Camelus dromedaries) FMDV antikorlarının araştırılması amaçlandı. Şubat-Temmuz 2019 tarihleri arasında Irak’ın üç vilayetinde (El-Necef, El-Muthanna ve El-Kadisiyah) farklı bölgelerden klinik olarak sağlıklı develerden (265 dişi ve 255 erkek) toplam 520 adet serum örneği toplandı ve develerin yaşına göre üç gruba ayrıldı. Tüm serum numuneleri, ELISA kullanılarak FMDV’nin yapısal olmayan proteinine (NSP) karşı antikor varlığı yönünden tarandı ve NSP pozitif serum örnekleri, sıvı fazı bloke eden ELISA (LPBE) ile FMDV serotipi O, A ve C’nin yapısal proteinlerine karşı antikor varlığının tespiti amacıyla tarandı. Sonuçlar, serumların %10’unun NSP antikorları için pozitif olduğunu ve FMDV serotip A’nın baskın olduğunu gösterdi. Ayrıca NSP pozitiflik oranının dişi develerden (%11.30) toplanan örneklerde erkek develerden (%8.60) daha fazla olduğu, NSP FMDV antikorlarının her yaştan devede bulunduğu belirlendi. Özetle, bu çalışma ile Irak’ta ilk kez develerin A ve O FMD serotiplerine daha duyarlı olduğu tespit edildi.

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