BİLAL AKYÜZ, KORHAN ARSLAN, DAVUT BAYRAM, M. Kaan İŞCAN

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Allelic frequency of kappa-casein, growth hormone and prolactin gene in holstein, brown swiss and simmental cattle breeds in Turkey

Bu çalışmada Türkiye’de yetiştirilen Holştayn (n=150), Simental (n=50) ve İsviçre Esmeri (n=50) sığır ırklarında kapa-kazein (κ-CN), büyüme hormonu (bGH) ve prolaktin hormonu (PRL) gen polimorfizimlerinin belirlenmesi amaçlanmıştır. Kapa-kazein-HindIII, PRL-RsaI ve bGH-AluI polimorfizimleri polimeraz zincir reaksiyonu ve restriksiyon parçacık büyüklük polimorfizimi (PCR-RFLP) ile belirlenmiştir. Kapa-kazein geni için 443 bç’lik bir bant, bGH geni için 223 bç’lik bir bant ve PRL geni için 156 bç’lik bir bant PZR ile yükseltgenmiştir. Bu çalışmada κ-CN için A ve B; bGH için L ve V; PRL için A ve B olarak olarak adlandırılan iki allel belirlenmiştir. En yüksek κ-CN-A allel frekansı HL ırkında (0.82), en yüksek κ-CN-B allel frekansı ise BS ırkında (0.55) belirlenmiştir. En yüksek bGH-L allel frekansı HL ırkında (0.85), en yüksek bGH-V allel frekansı ise S ırkında (0.34) belirlenmiştir. En yüksek PRL-A allel frekansı HL ırkında (0.87), en yüksek PRL-B allel frekansı ise BS ırkında (0.24) belirlenmiştir. Ki-kare sonuçlarına göre, incelenen ırklarda Harrdy-Weinberd dengesinden istatiksel olarak sapma sadece bGH lokusunda gözlenmiştir. Bu çalışma Türkiye’de yetiştirilen Holştayn, Simmental ve İsviçre Esmeri sığır ırklarında κ-CN, bGH ve PRL genotiplendirilmesinin yapıldığı ilk çalışmadır.
Anahtar Kelimeler:

somatotropin, Amerikan Esmer Irkı, Alacalı Sığır, Simmental, Türkiye, alleller, sığır, sığır ırkları, inek, süt sığırı, süt ineği, genler, genetik polimorfizm, kappa kazein, lokuslar, prolaktin

Türkiye’deki Holştayn, İsviçre esmeri ve simmental sığır ırklarında kapa-kazein, büyüme hormonu ve prolaktin genlerinin allel frekansları

The purpose of this study was to examine the kappa-casein (κ-CN), growth hormone (bGH) and prolactin hormone (PRL) gene polymorphisms in the Holstein (n=150), Simmental (n=50) and Brown Swiss (n=50) cattle breeds in Turkey. In order to determine the κ-CN-HindIII, PRL-RsaI and bGH-AluI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 443 bp fragment of κ-CN, a 223 bp fragment of bGH and a 156 bp fragment of PRL were amplified. In this study, two types of alleles, A and B for κ-CN, V and L for bGH, and A and B for PRL, were observed. The cattle breeds in which the highest frequencies of the alleles were estimated, were the HL breed (0.82) for κ-CN-A, the BS breed (0.55) for κ-CN-B, the HL breed (0.85) for bGH-L, the S breed (0.34) for bGH-V, the HL breed (0.87) for PRL-A, and the BS breed (0.24) for PRL-B. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined only for the bGH locus in the investigated breeds. The present study is the first report that examines three loci (κ-CN, bGH and PRL) in three cattle breeds of European origin (Holstein, Simmental and Brown Swiss) raised in Turkey.
Keywords:

somatotropin, American Brown Swiss, Holstein-Friesian, Simmental, Turkey, alleles, cattle, cattle breeds, cows, dairy cattle, dairy cows, genes, genetic polymorphism, kappa-casein, loci, prolactin,

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Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
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