Forutan SALEHINEZHAD, Hamidreza ESHRAGHI, Ali KADIVAR, Sadegh SHIRIAN, Ahmad ASGHARI, Ehsan AALI, Najmeh DAVOODIAN

Amelioration Effects of Vitamin E, Melatonin, L-carnitine, and Atorvastatin, on Destructive Effects of Busulfan in the Testes of Male Rats: A Gene Expression Evaluation

According to toxicity of various types of cancer treatments on different kind of cells with high division activities such as germ cells, antioxidants may protect these cells in testes against the toxic effects of the chemotherapeutic drugs. For this purpose, 24 h after busulfan treatment, 30 adult male wistar-rats were divided to six groups. Intra-peritoneally administrations of normal saline in control group and DMSO (as a busulfan solvent) in DMSO group were performed daily for 6 weeks beside the treatment contain vitamin E (Vit-E group), L-carnitine and melatonin (LM group), atorvastatin and melatonin (AM group), atorvastatin, L-carnitine, and melatonin (ALM group). After decapitation and removal of the testes, molecular evaluations were performed by the relative abundance measurement of DAZL, Bcl2, and Casp3 transcripts. The results of this study exhibited high level of expression of DAZL in Vit-E treated rats compared to control counterparts (P

Erkek Rat Testislerinde Busulfan Kaynaklı Hasara Karşı Vitamin E, Melatonin, L-karnitin ve Atorvastatin’in Koruyucu Etkisi: Gen Ekspresyonunun Değerlendirilmesi

Çeşitli kanser ilaçlarının toksisitelerine bağlı olarak eşey hücreleri gibi yüksek bölünme kapasitesine sahip çeşitli hücrelerde meydana gelen hasara karşı antioksidanlar koruyucu olabilir ve bu durum testis dokusunda kemoterapötik ilaçlarla oluşan hasarda etkili olabilir. Bu amaçla, 24 saat busulfan uygulaması sonucunda 30 ergin erkek Wistar rat altı gruba ayrıldı. İntraperitoneal olarak 6 hafta süresince DMSO grubuna fizyolojik tuzlu su ve DMSO (busulfan çözücüsü olarak) uygulandı. Diğer gruplarda busulfan uygulamasına ilave olarak E (Vit-E grubu), L-karnitin ve melatonin (LM grubu), atorvastatin ve melatonin (AM grubu), atorvastatin, L-karnitin ve melatonin (ALM grubu) uygulamaları gerçekleştirildi. Hayvanlarda dekapitasyonu takiben testis dokuları alındı ve DAZL, Bcl2, ve Casp3 miktarları karşılaştırmalı olarak moleküler açıdan değerlendirildi. Çalışma sonucu, Vit-E uygulanan ratlarda DAZL ekspresyonunun kontrol grubuna oranla daha fazla olduğu gözlemlendi (P

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