GENCAY TAŞKIN TAŞÇI, ZATİ VATANSEVER, Nilgün PARMAKSIZOĞLU AYDIN, NESLİHAN ÖLMEZ, ATİLA AKÇA, MÜKREMİN ÖZKAN ARSLAN, NERİMAN MOR, BARIŞ SARI

Prevalence and Molecular Characterization of Trypanosoma spp. in Domestic Geese (Anser anser domesticus) from the North-East Anatolia Region of Turkey

Avian trypanosomiosis is a protozoan disease transmitted by blood-sucking arthropods belonging to the Simuliidae, Culicidae, Ceratopogonidae, Hippoboscidae, and Dermanyssidae. Trypanosoma spp. are diagnosed by using microscopic and/or molecular methods. In this study, a total of 400 domestic geese blood samples were examined using nested PCR with the primers that amplify a gene fragment of 18S rRNA of Trypanosoma spp. The molecular prevalence of Trypanosoma spp. was determined as 50.25%. 18S rRNA amplicons from the two positive isolates were sequenced with the PCR primers. The obtained sequences from the two isolates exhibited 99.7% identity to each other. Sequence and blastn analyses revealed that two isolates were highly identical (99.7-100%) to the published 18S rRNA sequences of T. thomasbancrofti, T. gallinarum and T. avium from Xanthomyza phrygia (regent honeyeater) and Gallus gallus (the red junglefowl). This study provides the first molecular data on Trypanosoma generations in poultry from Turkey. The presence of Trypanosoma spp. was also firstly identified in domestic geese (Anser anser domesticus) in the world by molecular tools and genetic characterization of a short fragment of the 18S rRNA gene region has been achieved.

Türkiye’nin Kuzeydoğu Anadolu Bölgesinde Kazlarda (Anser anser domesticus) Trypanosoma spp. Prevalansı ve Moleküler Karakterizasyonu

Kanatlı trypanosomiosisi, Simuliidae, Culicidae, Ceratopogonidae, Hippoboscidae ve Dermanyssidae ailelerinde bulunan kan emen artropodlar tarafından bulaştırılan bir protozoan enfeksiyonudur. Trypanosoma türleri mikroskobik ve/veya moleküler yöntemlerle teşhis edilebilir. Bu çalışmada, Trypanosoma türlerinin 18S rRNA gen fragmanını çoğaltan primerler kullanılarak nested PCR ile toplam 400 kazın kan örneği incelenmiştir. Trypanosoma spp. prevalansı %50.25 olarak tespit edilmiştir. İki pozitif izolattan elde edilen 18S rRNA amplikonlarının PCR primerleri ile sekans analizi yaptırılmıştır. İki izolattan elde edilen sekanslar birbirleri ile %99.7 oranında identiklik göstermiştir. Sekans ve blastn analizleri, bu iki izolatın Xanthomyza phrygia (regent honeyeater) ve Gallus gallus’tan (kırmızı junglefowl) izole edilen T. thomasbancrofti, T. gallinarum ve T. avium’un 18S rRNA dizilimlerine çok benzer olduğunu (%99.7-100) ortaya koymuştur. Bu çalışma Türkiye’de kanatlılarda yayılış gösteren Trypanosoma türleriyle ilgili ilk moleküler verileri sağlamaktadır. Ayrıca Trypanosoma türlerinin evcil kazlarda (Anser anser domesticus) varlığı dünyada ilk olarak tespit edilmiş ve 18S rRNA gen bölgesinin kısa bir parçasının genetik karakterizasyonu sağlanmıştır.

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