Düşük Nişastalı Rasyonlarla Beslenen Geçiş Dönemindeki İneklerde Amilaz Enziminin Etkisi: 1. Laktasyon Performansı
Bu araştırmanın amacı geçiş dönemindeki ineklerin rasyonlarına amilaz enzimi ilavesinin kuru madde tüketimi ve laktasyon performansı üzerine etkisini incelemektir. Rasyonların nişasta düzeyi kuru madde esasına göre kuru dönemdeki hayvanlar için %15.5, laktasyon dönemindekiler için ise %19.5 olarak tespit edildi. Araştırmada birden fazla doğum yapmış 30 baş siyah alaca ırkı inekler rastgele amilaz (n=15) ve kontrol (n=15) gruplarına dağıtıldı. Araştırma kontrol ve amilaz (0.5 g Ronozyme RumiStar/kg toplam karma rasyon kuru madde) grupları şeklinde oluşturuldu. Deneme doğumdan önceki 21 gün ile doğumdan sonraki 84. günler arasında yürütüldü. Kuruda bulunan kontrol ve amilaz grubundaki rasyonların nişasta ve nötral deterjant fiber (NDF) içerikleri sırasıyla %15.5±0.5 ve %15.7±0.9 ile %42.6±1.1 ve %43.4±1.2 iken laktasyondakilerde ise %19.8±2.9 ve %19.4±0.5 ile %33.6±0.8 and %34.2±0.6 arasında tespit edildi. Doğum sonrası kuru madde tüketimi, organic madde, NDF, ham protein, nişasta düzeyleri bakımından gruplar arasında bir farklılık bulunmamıştır. Süt verimi uygulamadan etkilenmedi ancak sayısal olarak amilaz ile beslenen ineklerde 2 kg/ gün daha fazla süt elde edildi. Süt yağı, proteini ve laktoz düzeyleri denemeden etkilenmedi, ancak yağa, katı maddeye ve enerjiye gore düzeltilmiş süt verimleri amilaz ile beslenen ineklerde 2 kg/gün daha fazla süt üretildi. Yağa, katı maddeye ve enerjiye gore düzeltilmiş süt verimlerinin yeme dönüşüm oranları (kg/kg kuru madde tüketimi) amilaz ile beslenen ineklerde kontrol grubuna gore %5-6 daha yüksek bulundu. Sonuç olarak düşük nişasta içeriğine sahip rasyonlarla beslenen geçiş dönemindeki ineklerde amilaz enzimi ilavesinin yemden yararlanmayı iyileştirdiği, süt veriminde potansiyal bir artış sağlayabileceği; ancak kuru madde tüketimini etkilemediği tespiti yapılmıştır.
Effects of Exogenous Amylase in Transition Dairy Cows Fed LowStarch Diets: 1. Lactation Performance
The objective of this trial was to determine the effect of exogenous amylase during the transition period in dairy cows on dry matter intake and lactation performance. The effect of exogenous amylase supplementation on lactation diets with low starch concentration (19.5% of dry matter) and dry period diets with moderate starch concentration (15.5% of dry matter) was evaluated. A total of 30 multiparus Holstein cows were randomly assigned to two groups fed diets with (n=15) or without amylase (n=15). Treatments were granular amylase (0.5 g of Ronozyme RumiStar per kg of total mixed ration dry matter) or control. The research was conducted starting at 21 d prepartum until 84 d postpartum. Starch and neutral detergent fiber concentrations averaged 15.5±0.5% and 15.7±0.9%, 42.6±1.1% and 43.4±1.2% in close up diets and 19.8±2.9% and 19.4±0.5%, 33.6±0.8% and 34.2±0.6% in lactation diets for control and amylase, respectively. Dry matter intake, milk yield and composition were evaluated for differences between treatments. Postpartum intakes of dry matter (DMI) and organic matter (OM), neutral detergent fiber (NDF), crude protein (CP), and starch intake were unaffected by treatment. Milk yield was not influenced by treatment, but numerically greater by 2.0 kg/d for cows fed amylase compared with control diet. The percentages of milk fat, protein and lactose were not impressed by treatment, however fat-, solid-, and energy-corrected milk were 2 kg/d greater for cows fed amylase diet than for cows fed control diet. Fat-, solid-, and energy-corrected milk feed conversions (kg/kg DMI) were 5 to 6% greater for cows fed amylase diet than for cows fed control diet (P
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- 1. Nafikov RA, Beitz DC: Carbohydrate and lipid metabolism in farm animals. J Nutr, 137 (3): 702-705, 2007. DOI: 10.1093/jn/137.3.702
- 2. NRC: Nutrients Requirements of Dairy Cattle. 7th rev. ed., National Academy Press Washington, DC, USA; 2001.
- 3. Gencoglu H, Shaver RD, Steinberg W, Ensink J, Ferraretto LF, Bertics SJ, Lopes JC, Akins MS: Effect of feeding a reduced-starch diet with or without amylase addition on lactation performance in dairy cows. J Dairy Sci, 93 (2): 723-732, 2010. DOI: 10.3168/jds.2009-2673
- 4. Cetin I, Turkmen II, Kara C, Orman A, Sen E: Improved lactational performance in dairy cows supplemented with methionine or rumenprotected choline during the transition period. Kafkas Univ Vet Fak, 24 (2): 289-293, 2018. DOI: 10.9775/kvfd.2017.18854
- 5. Kovacevic Z, Cincovic MR, Stojanovic D, Belic B, Jezdimirovic M, Djokovic R, Davidov I: Influence of ketoprofen application on lipid mobilization, ketogenesis and metabolic status in cows during early lactation. Kafkas Univ Vet Fak, 22 (1): 7-12, 2016. DOI: 10.9775/ kvfd.2015.13479
- 6. Firkins JL, Eastridge ML, St-Pierre NR, Noftsger SM: Effects of grain variability and processing on starch utilization by lactating dairy cattle. J Anim Sci, 79 (Suppl E): E218-E238, 2001. DOI: 10.2527/jas2001.79ESupplE218x
- 7. Ferraretto LF, Crump PM, Shaver RD: Effect of cereal grain type and corn grain harvesting and processing methods on intake, digestion, and milk production by dairy cows through a meta-analysis. J Dairy Sci, 96, 533-550, 2013. DOI: 10.3168/jds.2012-5932
- 8. Ferraretto LF, Shaver RD: Meta-analysis: Effect of corn silage harvest practices on intake, digestion, and milk production by dairy cows. Prof Anim Sci, 28 (2): 141-149, 2012. DOI: 10.15232/S1080-7446(15)30334-X
- 9. Hristov AN, McAllister TA, Cheng KJ: Stability of exogenous polysaccharide-degrading enzymes in the rumen. Anim Feed Sci Technol, 76 (1-2): 161–168, 1998. DOI: 10.1016/S0377-8401(98)00217-X
- 10. Klingerman CM, Hu W, McDonell EE, Der Bedrosian MC, Kung L: An evaluation of exogenous enzymes with amylolytic activity for dairy cows. J Dairy Sci, 92 (3): 1050-1059, 2009. DOI: 10.3168/jds.2008-1339
- 11. DiLorenzo N, Smith DR, Quinn MJ, May ML, Ponce CH, Steinberg W, Engstrom MA, Galyean ML: Effects of grain processing and supplementation with exogenous amylase on nutrient digestibility in feedlot diets. Livest Sci, 137 (1-3): 178-184, 2011. DOI: 10.1016/j. livsci.2010.11.003
- 12. Ferraretto LF, Shaver RD, Espineira M, Gencoglu H, Bertics SJ: Influence of a reduced-starch diet with or without exogenous amylase on lactation performance by dairy cows. J Dairy Sci, 94 (3): 1490–1499, 2011. DOI: 10.3168/jds.2010-3736
- 13. Weiss WP, Steinberg W, Engstrom MA: Milk production and nutrient digestibility by dairy cows when fed exogenous amylase with coarsely ground dry corn. J Dairy Sci, 94 (5): 2492–2499, 2011. DOI: 10.3168/ jds.2010-3766
- 14. Jung S, Vogel K: Determination of Ronozyme RumiStar AlphaAmylase Activity in Feed and Per Se Samples. DSM Nutritional Products Ltd., Basel, Switzerland: Regulatory Report No.2500706, 2008. 15. AOAC: Official Methods of Analysis. 17th ed., Gaithersburg, Maryland, USA: AOAC International; 2002.
- 16. Van Soest PJ, Robertson JB, Lewis BA: Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci, 74 (10): 3583-3597, 1991. DOI: 10.3168/jds. S0022-0302(91)78551-2
- 17. Bal MA, Shaver RD, Jirovec AG, Shinners KJ, Coors JG: Crop processing and chop length of corn silage: Effects on intake, digestion, and milk production by dairy cows. J Dairy Sci, 83 (6): 1264-1273, 2000. DOI: 10.3168/jds.S0022-0302(00)74993-9
- 18. Kononoff PJ, Heinrichs AJ, Buckmaster DR: Modification of the Penn State forage and total mixed ration particle separator and the effects of moisture content on its measurements. J Dairy Sci, 86 (5): 18581863, 2003. DOI: 10.3168/jds.S0022-0302(03)73773-4
- 19. Noziere P, Steinberg W, Silberberg M, Morgavi DP: Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets. J Dairy Sci, 97 (4): 2319-2328, 2014. DOI: 10.3168/jds.2013-7095
- 20. DeFrain JM, Hippen AR, Kalscheur KF, Tricarico JM: Effects of dietary α-amylase on metabolism and performance on transition cows. J Dairy Sci, 88 (12): 4405-4413, 2005. DOI: 10.3168/jds.S0022-0302(05)73127-1
- 21. McCarthy MM, Engstrom MA, Azem E, Gressley TF: The effect of an exogenous amylase on performance and total-tract digestibility in lactating dairy cows fed a high-byproduct diet. J Dairy Sci, 96 (5): 30753084, 2013. DOI: 10.3168/jds.2012-6045
- 22. Tricarico JM, Johnston JD, Dawson KA, Hanson KC, McLeod KR, Harmon DL: The effects of an Aspergillus oryzae extract containing alpha-amylase activity on ruminal fermentation and milk production in lactating Holstein cows. Anim Sci, 81 (3): 365-374, 2005. DOI: 10.1079/ ASC50410365
- 23. Andreazzi ASR, Pereira MN, eis RB, Pereira RAN, Júnior NNM, Acedo TS, Hermes RG, Cortinhas CS: Effect of exogenous amylase on lactation performance of dairy cows fed a high-starch diet. J Dairy Sci, 101 (8): 7129-7207, 2018. DOI: 10.3168/jds.2017-14331
- 24. Harrison GA, Tricarico JM: Effects of an Aspergillus oryzae extract containing α-amylase activity on lactational performance in commercial dairy herds. Prof Anim Sci, 23 (3): 291-294, 2007. DOI: 10.15232/S10807446(15)30976-1
- 25. Seymour WM, Campbell DR, Johnson ZB: Relationships between rumen volatile fatty acid concentrations and milk production in dairy cows: A literature study. Anim Feed Sci Technol, 119 (1-2): 155-169, 2005. DOI: 10.1016/j.anifeedsci.2004.10.001
- 26. West, JW: Effects of heat-stress on production in dairy cattle. J Dairy Sci, 86 (6): 2131-2144, 2003. DOI: 10.3168/jds.S0022-0302(03)73803-X
- 27. Orman A, Ogan MM: Environmental factors affecting milk and milk fat yields of Holstein cows. Indian Vet J 83 (5): 623-625, 2008.
- 28. Vargas-Rodriguez CF, Engstrom M, Azem E, Bradford BJ: Effects of dietary amylase and sucrose on productivity of cows fed low-starch diets. J Dairy Sci, 97 (7): 4464-4470, 2014. DOI: 10.3168/jds.2013-7845