Piliç Kanatlarından İzole Edilen Salmonella spp.’nin Antibiyotik Direnci
Gıda amaçlı hayvan yetiştiriciliğinde antibiyotik kullanımı, gıda zinciri yoluyla insana geçiş yapan, antibiyotiklere dirençli bakterilerin en önemli kaynağı konumundadır. Günümüzde antimikrobiyal ilaçlara dirençli Salmonella spp. tüm ülkelere yayılmış durumdadır. Bu çalışma ile kanatlı etlerindeki Salmonella prevalansı ve izolatların antimikrobiyal direnç durumları araştırılmıştır. İncelenen 200 piliç kanat paketinden 102 tanesi (%51) pozitif olarak değerlendirilmiş ve konfirme edilen 336 izolattan 200’ünün antibiyotik duyarlılık durumları belirlenmiştir. Buna göre tüm izolatların ortalama 0.371 çoklu antibiyotik direnci (MAR) indeksi ile çoklu antibiyotik direncine sahip olduğu görülmüştür. İzolatların hiçbiri amoxicillin/clavulanate ve cefoxitin’e direnç göstermemiştir. Yalnızca 2 izolatın imipenem’e dirençli olduğu saptanmıştır. Asıl direncin nalidixic acid (%95), trimethoprim/sulfomethoxazole (%92), tetracycline (%92), streptomycin (%90) ve trimethoprim (%81) antibiyotiklerine karşı şekillendiği gözlenmiştir. Her ne kadar sadece 4 izolat ciprofloxacin’e karşı tam dirençli olsa da orta düzeydeki direnç oranının yüksek olduğu (%92) ortaya konmuştur. İzolatlar ayrıca gentamisin (%7), cefoperazone (%2), ampicillin (%24), chloramphenicol (%24), cephazolin (%7) ve cefotaxime (%39)’e karşı direnç geliştirmiştir. Çalışmamızın bulguları Salmonella spp.’nin yüksek prevalansı ve antibiyotik direncindeki artış nedeniyle halk sağlığına yönelik önemli bir tehdit oluşturduğuna dikkati çekmiştir.
Antibiotic Resistance of Salmonella spp. Isolated from Raw Chicken Wings
The use of antibiotics in food animals creates an important source of antimicrobial resistant bacteria that can spread to humans through thefood chain. Strains of Salmonella spp. with resistance to antimicrobial drugs are now widespread in all countries. The present study analysedthe prevalence and antimicrobial resistance of Salmonella spp. isolates in raw chicken wings. Out of 200 fresh raw chicken wing packages102 (51%) samples were positive. Antibiotic resistance test was performed on 200 isolates out of 336 after being confirmed. All the isolatesshowed multiple resistance against the antibiotics investigated with the average 0.371 multiple antibiotic resistance (MAR) index. None ofthe isolates were resistant to amoxicillin/clavulanate and cefoxitin. Only 2 isolates showed intermediate resistance to imipenem. The majorresistance was observed against nalidixic acid (95%), trimethoprim/sulfomethoxazole (92%), tetracycline (92%), streptomycin (90%) andtrimethoprim (81%). Even though only 4 isolates were resistant against ciprofloxacin, high percentage of intermediate resistance (92%)was detected. Some of the isolates were also resistant to gentamicin (7%), cefoperazone (2%), ampicillin (24%), chloramphenicol (24%),cephazolin (7%) and cefotaxime (39%). According to our results high prevalence and the increase in antibiotic resistant Salmonella spp. is ofconcern and constitutes a threat to public health.
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- aradat ZW, Abedel Hafiz L, Ababneh MM, Ababneh QO, Al Mousa
W, Al-Nabulsi A, Osaili TM, Holley R: Comparative analysis of virulence
and resistance profiles of Salmonella enteritidis isolates from poultry meat
and foodborne outbreaks in northern Jordan. Virulence, 5 (5): 601-610,
2014. DOI: 10.4161/viru.29002
- Duffy G, Cloak OM, O’Sullivan MG, Guillet A, Sheridan JJ, Blair
IS, McDowell DA: The incidence and antibiotic resistance profiles of
Salmonella spp. on Irish retail meat products. Food Microbiol, 16, 623-631,
1999. DOI: 10.1006/fmic.1999.0278
- Nguyen DTA, Kanki M, Nguyen PD, Le HT, Ngo PT, Tran DNM,
Le NH, Dang CV, Kawai T, Kawahara R, Yonogi S, Hirai Y, Jinnai M,
Yamasaki S, Kumeda Y, Yamamoto Y: Prevalence, antibiotic resistance,
and extended-spectrum and AmpC b-lactamase productivity of
Salmonella isolates from raw meat and seafood samples in Ho Chi Minh
City, Vietnam. Int J Food Microbiol, 236, 115-122, 2016. DOI: 10.1016/j.
ijfoodmicro.2016.07.017
- Collignon P, Powers JH, Chiller TM, Aidara-Kane A, Aarestrup
FM: World health organization ranking of antimicrobials according to
their importance in human medicine: A critical step for developing risk
management strategies for the use of antimicrobials in food production
animals. Clin Infect Dis, 49 (1): 132-141, 2009. DOI: 10.1086/599374
- Kaya İB, Şahan Yapıcıer Ö, Akan M, Diker KS: Class 1 integrons and
the antibiotic resistance profile of Salmonella Infantis strains from broiler
chickens. Kafkas Univ Vet Fak Derg, 23 (5): 803-807, 2017. DOI: 10.9775/
kvfd.2017.17821
- Alcaine SD, Molla L, Nugen SR, Kruse H: Results of a pilot antibiotic
resistance survey of Albanian poultry farms. J Glob Antimicrob Resist, 4,
60-64, 2016. DOI: 10.1016/j.jgar.2015.11.003
- Zhu Y, Lai H, Zou L, Yin S, Wang C, Han X, Xia X, Hu K, He L, Zhou
K, Chen S, Ao X, Liu S: Antimicrobial resistance and resistance genes in
Salmonella strains isolated from broiler chickens along the slaughtering
process in China. Int J Food Microbiol, 259, 43-51, 2017. DOI: 10.1016/j.
ijfoodmicro.2017.07.023
- Yildirim Y, Gonulalan Z, Pamuk S, Ertas N: Incidence and antibiotic
resistance of Salmonella spp. on raw chicken carcasses. Food Res Int, 44 (3):
725-728, 2011. DOI: 10.1016/j.foodres.2010.12.040
- Asif M, Rahman H, Qasim M, Khan TA, Ullah W, Jie Y: Molecular
detection and antimicrobial resistance profile of zoonotic Salmonella
enteritidis isolated from broiler chickens in Kohat, Pakistan. J Chin Med
Assoc, 80 (5): 303-306, 2017. DOI: 10.1016/j.jcma.2016.11.007
- Ziech RE, Lampugnani C, Perin AP, Sereno MJ, Sfaciotte RAP,
Viana C, Soares VM, Pinto JPAN, Bersot LS: Multidrug resistance and
ESBL-producing Salmonella spp., isolated from broiler processing plants.
Braz J Microbiol, 47 (1): 191-195, 2016. DOI: 10.1016/j.bjm.2015.11.021
- Álvarez-Fernández E, Alonso-Calleja C, García-Fernández C,
Capita R: Prevalence and antimicrobial resistance of Salmonella serotypes
isolated from poultry in Spain: Comparison between 1993
and 2006. Int J Food Microbiol, 153 (3): 281-287, 2012. DOI: 10.1016/j.
ijfoodmicro.2011.11.011
- Krumperman PH: Multiple antibiotic resistance indexing of
Escherichia coli to identify high-risk sources of faecal contamination of
foods. Appl Environ Microbiol, 46 (1): 165-170, 1983.
- EUCAST, European Society of Clinical Microbiology and Infectious
Diseases: European committee on antimicrobial susceptibility testing
guidelines for detection resistance mechanisms and specific resistances
of clinical and/or epidemiological importance. 2013.
- CLSI: Clinical and Laboratory Standards Institute Performance
standards for antimicrobial susceptibility testing: twenty-fourth
informational supplement. CLSI document M100-S24. Wayne, PA: CLSI,
2014.
- ISO, The International Organization of Standardizations: Microbiology
of food animal feeding stuffs-horizontal method for the detection
Salmonella spp. International Standard, ISO 6579.
- Su LH, Chiu CH, Chu C, Ou JT: Antimicrobial resistance in nontyphoid
Salmonella serotype: A global challenge. Clin Infect Dis, 39 (4): 546-551,
2004. DOI: 10.1086/422726
- WHO: Global Strategy for containment of antimicrobial Resistance.
WHO, Geneva. WHO/CDC/CSR/DR/2001.2, 2001.
- Smith DL, Harris AD, Johnson JA, Silbergeld EK, Morris JG Jr: Animal
antibiotic use has an early but important impact on the emergence of
antibiotic resistance in human commensal bacteria. Proc Natl Acad Sci
USA, 99 (9): 6434-6439, 2002. DOI: 10.1073/pnas.082188899
- Johnson JR, Kuskowski MA, Menard M, Gajewski A, Xercavins M,
Garau J: Similarity between human and chicken Escherichia coli isolates
in relation to Ciprofloxacin resistance status. J Infect Dis, 194, 71-78, 2006.
DOI: 10.1086/504921
- Aarestrup FM, Wegener HC, Collignon P: Resistance in bacteria of
the food chain: Epidemiology and control strategies. Expert Rev Anti Infect
Ther, 6 (5): 733-750, 2008. DOI: 10.1586/14787210.6.5.733
- Crump JA, Sjölund-Karlsson M, Gordon MA, Parry CM: Epidemiology,
clinical presentation, laboratory diagnosis, antimicrobial resistance,
and antimicrobial management of invasive Salmonella infections. Clin
Microbiol Rev, 28 (4): 901-937, 2015. DOI: 10.1128/CMR.00002-15
- White DG, Zhao S, Sudler R, Ayers S, Friedman S, Chen S,
McDermott PF, McDermott S, Wagner DD, Meng J: The isolation of
antibiotic-resistant Salmonella from retail ground meats. N Engl J Med,
345, 1147-1154, 2001. DOI: 10.1056/NEJMoa010315
- FAO/WHO Food and Agriculture Organisations and United
Nations/World Health Organisation: Salmonella and campylobacter
Chicken Meat: Meeting Report, Microbiological Risk Assessment Series,
No: 19, Rome, 56 pp, 2009.
- Hassan AHA, Salam HSH, Abdel-Latef GK: Serological identification
and antimicrobial resistance of Salmonella isolates from broiler carcasses
and human stools in Beni-Suef, Egypt. Beni-Suef Univ J Appl Sci, 5, 202-207,
2016. DOI: 10.1016/j.bjbas.2016.04.002