Hidayet TUTUN, Ender YARSAN, Görkem KISMALI, Sedat SEVİN, Emine Kübra BİLİR

Rhododendron ponticum L. Extractının Prostat Karsinomu ve Adenokarsinoma Hücre Hatları (DU145, PC3) Üzerindeki Sitotoksik Etkileri

Yangı, ağrı, soğuk algınlığı, astım, deri ve gastrointestinal hastalıkların tedavisinde geleneksel olarak kullanılan Ormangülü türleri (Rhododendron) dünya çapında yaygın olarak dağılım göstermektedir. Türkiye’nin Karadeniz bölgesinde dağılmış Ormangülünün (Rhododendron ponticum L.) nektarından elde edilen delibal, toksik diterpen olan grayanotoksinleri içerir. Delibalın toksisitesinden sorumlu olan grayanotoksinler, çoğunlukla grayanotoksinler I ve III, yapraklarda, çiçeklerde ve nektarlarda bulunur. Bu çalışmanın amacı, orman gülü ekstraktının prostat karsinom hücre hatları üzerine in vitro sitotoksik etkilerini araştırmaktır. Çiçeklenme döneminde Ordu Altınordu ilçesinden toplanan orman gülü, uygun koşullar altında kurutuldu, distile su ile ekstrakte ve sonrasında liyofilize edildi. İnsan prostat kanseri (DU145) ve insan prostat adenokarsinoması (PC3) hücre hatlarına karşı orman gülü ekstraktının sitotoksik etkinliği MTT ve Nötral Kırmızı testleri ile değerlendirildi. Orman gülü ekstraktının doza bağımlı bir sitotoksik etki yaptığı tespit edildi. Ekstraktın IC50’si, DU145 ve PC3 için MTT analizinde, sırasıyla 283.3 ve 169.9 µg/mL ve Nötral kırmızı analizinde, sırasıyla 307.6 ve 346.0 µg/mL olarak bulundu. Orman gülü, prostat karsinom hücreleri üzerinde sitotoksik etkilere sahip olması, antikanserojenik aktivite için potansiyel bir terapötik madde olabileceğini düşündürmektedir.

Cytotoxic Effects of Rhododendron ponticum L. Extract on Prostate Carcinoma and Adenocarcinoma Cell Line (DU145, PC3)

Rhododendron species, having been used in traditional medicine for the treatment of inflammation, pain, cold, asthma, skin andgastro-intestinal disease, are distributed widely around the world. Mad honey obtained from the nectar of common rhododendron(Rhododendron ponticum L.), which is distributed throughout Black Sea region of Turkey, contains grayanotoxins, which are toxicditerpenes. The grayanotoxins, mostly grayanotoxins I and III, which are present in leaves, flowers and nectar responsible for toxicityof mad honey. The aim of this study was to investigate in vitro cytotoxic effects of the extract on prostate carcinoma cell lines. Duringthe flowering period of common rhododendrons gathered from the Altinordu District of Ordu and dried under suitable conditions,extracted with distilled water and lyophilized. The cytotoxic activity of the extract of common rhododendron against human prostatecarcinoma (DU145) and human prostate adenocarcinoma (PC3) cell lines by using the MTT and Neutral Red assay was evaluated. Itwas determined that the extract of common rhododendron had a dose-dependent cytotoxic effect. IC50 of the extract was found tobe 283.3 and 169.9 µg/mL in MTT assay, and 307.6 and 346.0 µg/mL in Neutral Red assay for DU145 and PC3 respectively. The fact thatcommon rhododendron has cytotoxic effects on prostate carcinoma cells suggests that it may be a potential therapeutic agent foranticarcinogenic activity.

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