NEBAHAT BİLGE, LEYLA VATANSEVER, ÇİĞDEM SEZER

Antibiotic Resistance of Salmonella spp. Isolated from Raw Chicken Wings

The use of antibiotics in food animals creates an important source of antimicrobial resistant bacteria that can spread to humans through the food chain. Strains of Salmonella spp. with resistance to antimicrobial drugs are now widespread in all countries. The present study analysed the prevalence and antimicrobial resistance of Salmonella spp. isolates in raw chicken wings. Out of 200 fresh raw chicken wing packages 102 (51%) samples were positive. Antibiotic resistance test was performed on 200 isolates out of 336 after being confirmed. All the isolates showed multiple resistance against the antibiotics investigated with the average 0.371 multiple antibiotic resistance (MAR) index. None of the isolates were resistant to amoxicillin/clavulanate and cefoxitin. Only 2 isolates showed intermediate resistance to imipenem. The major resistance was observed against nalidixic acid (95%), trimethoprim/sulfomethoxazole (92%), tetracycline (92%), streptomycin (90%) and trimethoprim (81%). Even though only 4 isolates were resistant against ciprofloxacin, high percentage of intermediate resistance (92%) was detected. Some of the isolates were also resistant to gentamicin (7%), cefoperazone (2%), ampicillin (24%), chloramphenicol (24%), cephazolin (7%) and cefotaxime (39%). According to our results high prevalence and the increase in antibiotic resistant Salmonella spp. is of concern and constitutes a threat to public health.

Piliç Kanatlarından İzole Edilen Salmonella spp.’nin Antibiyotik Direnci

Gıda amaçlı hayvan yetiştiriciliğinde antibiyotik kullanımı, gıda zinciri yoluyla insana geçiş yapan, antibiyotiklere dirençli bakterilerin en önemli kaynağı konumundadır. Günümüzde antimikrobiyal ilaçlara dirençli Salmonella spp. tüm ülkelere yayılmış durumdadır. Bu çalışma ile kanatlı etlerindeki Salmonella prevalansı ve izolatların antimikrobiyal direnç durumları araştırılmıştır. İncelenen 200 piliç kanat paketinden 102 tanesi (%51) pozitif olarak değerlendirilmiş ve konfirme edilen 336 izolattan 200’ünün antibiyotik duyarlılık durumları belirlenmiştir. Buna göre tüm izolatların ortalama 0.371 çoklu antibiyotik direnci (MAR) indeksi ile çoklu antibiyotik direncine sahip olduğu görülmüştür. İzolatların hiçbiri amoxicillin/clavulanate ve cefoxitin’e direnç göstermemiştir. Yalnızca 2 izolatın imipenem’e dirençli olduğu saptanmıştır. Asıl direncin nalidixic acid (%95), trimethoprim/sulfomethoxazole (%92), tetracycline (%92), streptomycin (%90) ve trimethoprim (%81) antibiyotiklerine karşı şekillendiği gözlenmiştir. Her ne kadar sadece 4 izolat ciprofloxacin’e karşı tam dirençli olsa da orta düzeydeki direnç oranının yüksek olduğu (%92) ortaya konmuştur. İzolatlar ayrıca gentamisin (%7), cefoperazone (%2), ampicillin (%24), chloramphenicol (%24), cephazolin (%7) ve cefotaxime (%39)’e karşı direnç geliştirmiştir. Çalışmamızın bulguları Salmonella spp.’nin yüksek prevalansı ve antibiyotik direncindeki artış nedeniyle halk sağlığına yönelik önemli bir tehdit oluşturduğuna dikkati çekmiştir.

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