Zeynep ERDEM, SÜHEYLA TÜRKYILMAZ

Molecular typing of methicillin resistant staphylococcus aureus strains isolated from cows and farm workers

Bu çalışma mastitisli sığır sütleri ile bu sığırlar ve çiftlik çalışanlarının nazal sıvaplarından metisilin dirençli Staphylococcus aureus (MRSA) izolasyon ve identifikasyonu, izolatların moleküler özelliklerinin ve Panton–Valentine Leucocidin (PVL) genlerinin varlığının incelenmesi amaçlandı. Elli altı sığırdan 145 mastitisli süt ile 56 sığır ve 34 çiftlik çalışanına ait nazal sıvaplar alındı. MRSA izolatlarının moleküler özellikleri itilmiş alan jel elektroforezisi (PFGE), stafilokokal protein A (spa), çok lokuslu dizi tiplendirme (MLST) ve stafilokokal kromozom kaset mec (SCCmec) tiplendirmesi ortaya konuldu. Tüm suşlar PVL genleri bakımından negatif idi. Toplam 12 MRSA identifikasyonu yapıldı. PFGE analizi sonucunda 12 MRSA izolatında pulsotip A-D olamak üzere dört pulsotip belirlendi. Pulsotip A olan suşların (n=7) sekans tipi (ST) tipi ST 239, spa tipi t030 olarak bulunurken biri hariç hepsinin SCCmec III taşıdığı tespit edildi. Pulsotip B (n=3) ST1294, t459 ve SCCmec Tip III; pulsotip C (n=1) ST1940, t660, SCCmec Tip III ve pulsotip D (n=1) ST737, t542, SCCmec tip II olarak belirlendi. Çalışma sonucunda, Aydın yöresinde sığır süt, insan ve sığır burun sıvaplarından izole edilmiş olan en yaygın spa tipi t030, MLST tipinin ise ST 239 olduğu belirlendi.

Sığırlardan ve sektör çalışanlarından izole edilen metisilin dirençli stafilokokların moleküler tiplendirmesi

The aims of this study were to isolation and identification of methicillin resistant Staphylococcus aureus (MRSA) from mastitic cow milk and nasal swabs of cows and related workers, investigation presence of Panton-Valentine leucocidin (PVL) genes and molecular characteristics of these isolates. One hundred forty five mastitic milk samples were obtained from of 56 cows and nasal swabs were collected from these cows and 34 farm workers. MRSA isolates were recovered by pulsed field gel electrophoresis (PFGE), staphylococcal chromosomal cassette (SCC) mec typing, staphylococcal protein A (spa) typing, multilocus sequence typing (MLST). A total of 12 MRSA were identified. All strains were PVL genes negative. PFGE analysis of all 12 MRSA isolates produced four distinct pulsotypes (PT) designated as PT A-D. The PT A (7 strains) detected sequence type (ST) were ST 239, spa type t030 and SCCmec III except one. The PT B (3 strains) were ST1294, t459 and SCCmec III, the PT C (1 strain) was ST1940, t660, SCCmec III detected, the PT D (1 strain) was ST737, t542, SCCmec II. Consequently, it can be said that t030 and ST239 was the most common spa and MLST types which isolated from cow milk and nasal swabs and nasal swabs from workers of these farms in Aydın region, Turkey.

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