MUZAFFER TAŞ, Berna Güney SARUHAN, Doğan KURT, Beran YOKUŞ, MUZAFFER DENLİ

Protective role of lycopene on aflatoxin B1 induced changes sperm characteristics and testicular damages in rats

Bu çalışma, likopen uygulamasının aflatoksin B1 (AFB1) uygulanmış ratların üreme sistemi, sperm kalitesi ve oksidatif stres üzerine olan önleyici etkilerini saptamak amacıyla yürütülmüştür. Çalışmada, toplam 8 haftalık yaşta ve ortalama 180-220 g canlı ağırlığa sahip toplam 28 adet erkek Wistar Albino ratlar kullanıldı. Ratlar rastgele 4 eşit gruba ayrıldı. Gruplar; Kontrol, Likopen grubu (10 mg/kg CA/gün), AFB1 grubu (2.5 mg/kg CA, tek doz ip.) ve Likopen(10 mg/kg CA/gün) + AFB1 (2.5 mg/kg CA, tek doz ip.) şeklinde oluşturulmuştur. Toplam 15 gün süren araştırma sonunda, testis ve spermatozoa özellikleri ile testiküler histoloji ve oksidasyon özellikleri incelenmiştir. AFB1 ve Kontrol grubları, motilite ve toplam anormal spermatozoon sayısı özellikleri bakımından karşılaştırıldığında, AFB1 uygulanan hayvanların motilitesinde önemli düzeyde azalma ve toplam anormal spermatozoon sayısında ise artış saptanmıştır (P

Anahtar Kelimeler:

aflatoksin, hayvan modelleri, hastalık modelleri, epididimis, doku bilimi, sıçan, spermatozoa, testis, testis hastalıkları

Ratlarda aflatoksin B1’in spermatozoa özellikleri ve testislerde yarattığı hasar üzerine likopenin koruyucu etkisi

The aim of this study was to investigate if lycopene could diminish the adverse effects of aflatoxin B1 (AFB1) on sperm characteristics, the testes, epididymis and oxidative stress in rats. A total of 28 adult male Wistar-Albino rats (8 weeks old weighing 180-220 g) were divided into four groups; Controls, lycopene treated rats (10 mg/kg BW, daily by gavage), AFB1 treated rats (2.5 mg/kg BW, single dose intra peritonal) and lycopene (10 mg/kg BW, daily by gavage) + AFB1 (2.5 mg/kg BW, single dose intra peritonal) treated rats. Traits of reproductive organs (testes and sperm characteristics, testicular histological findings and the testicular tissue oxidative status) were determined after 15 days of treatment. The sperm motility was significantly decreased while the rate of total abnormal sperms was significantly increased in rats treated with AFB1 alone compared to their levels in controls (P<0.001). The level of testes malondialdehyde (MDA) in rats treated with AFB1 was significantly higher than its level in controls (P<0.001). Treatment with AFB1 significantly decreased testes diameter and seminifer epithelium thickness (P<0.0001) when compared with the others. Similarly, the thickness of the germinative cell layer at seminifer tubul (ST) in rats treated with AFB1 was significantly smaller than controls. Treatment with lycopene significantly increased sperm motility (P<0.001) and alleviated the many negative effects of AFB1 on sperm characteristic and testicular damage in rats. In conclusion our results showed that consumption of 10 mg of lycopene/ BW given by gavage protected the rats to the toxicity of AFB1 when it is administrated as a single dose intraperitoneally.

Keywords:

aflatoxins, animal models, disease models, epididymis, histology, rats, spermatozoa, testes, testicular diseases,

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