Ebru BEYTUT, NADİDE NABİL KAMİLOĞLU, AYSEL GÜVEN

Lipid peroxidation and antioxidant levels in the lungs and associated lymph nodes of cattle with anthracosis

Sunulan çalışmada, antrakozisli sığırların akciğer, mediastinal ve bronşial lenf düğümlerinde lipid peroksidasyonu ve antioksidan düzeyleri belirlendi. Çalışmada, Kars yöresinin değişik köylerinden getirilen ve bölgedeki iki mezbahada kesilen farklı ırk ve yaşlardaki 600 sığıra ait akciğer ve ilgili lenf düğümleri antrakozis yönünden incelendi. Makroskobik muayenede siyah pigmentasyon gözlenen 20 akciğer ve bunlara ait lenf düğümlerinden laboratuar analizleri için doku örnekleri alındı. Ayrıca, makroskobik olarak pigmentasyon bulunmayan 20 sığıra ait akciğer ve ilgili lenf düğümleri ise kontrol olarak kullanıldı. Akciğer ve lenf düğümlerinde glutatyon peroksidaz (GSH-Px; EC 1.11.1.9) aktivitesi ile glutatyon (GSH), A ve E vitaminleri, b-karoten ve tiyobarbiturik asit substrat (TBARS) düzeyleri belirlendi. Antrakozisli sığırların akciğer ve lenf düğümlerinde TBARS düzeylerinin kontrollere göre istatistiksel olarak yüksek (p

Anahtar Kelimeler:

sığır, savunma mekanizması, lipit peroksidasyonu, akciğerler, lenf düğümleri, antioksidanlar

Antrakozisli sığırların akciğer ve ilgili lenf düğümlerinde lipid peroksidasyonu ve antioksidan düzeyleri

The aim of the present study was to examine lipid peroxidation and antioxidant levels in the lungs, mediastinal and bronchial lymph nodes of cattle with anthracosis. In the study, the lungs and associated lymph nodes of 600 cattle of various species and ages, brought from different villages in the Kars region and slaughtered in two local abattoirs, were examined for anthracosis. Upon gross examination, anthracosis were detected in the lungs and their associated lymph nodes of 20 (3.3 %) cattle and specimens were taken for laboratory analysis. Additionally, samples from the lungs and the lymph nodes with no pigmentation of 20 cattle were used as control. The levels of thiobarbituric acid reactive substance (TBARS), vitamin E, A, b-carotene, glutathione (GSH) and the activity of glutathione peroxidase (GSH-Px; EC 1.11.1.9) in the lungs and the lymph nodes were determined. TBARS levels in the lungs and the lymph nodes of cattle with anthracosis were significantly (p<0.001) increased as compared to the controls. Vitamin E, A and b-carotene levels in the lungs and the lymph nodes of the animals with anthracosis were also significantly (p<0.05, p<0.01, respectively) decreased. GSH-Px activity and GSH levels in the lungs and the lymph nodes with anthracosis were also significantly (p<0.05) decreased as compared to the controls. These results suggested that increase in lipid peroxidation of the lungs and the lymph nodes were most likely produced by the suppression of antioxidant defence abilities in cattle with anthracosis.

Keywords:

cattle, defence mechanisms, lipid peroxidation, lungs, lymph nodes, antioxidants,

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