MEHMET ÇİTİL, MEHMET TUZCU, ABDULLAH DOĞAN, ERDOĞAN UZLU, EMİNE ATAKİŞİ, AYŞE KANICI, METEHAN UZUN, MAHMUT KARAPEHLİVAN, MAHMUT KARAPEHLİVAN

Kronik aflatoksikozis oluşturulan bıldırcınlarda (Coturnix coturnix japonica) Ll-Karnitinin, biyokimyasal, hematolojik ve patolojik parametreler üzerine etkilerinin araştırılması

Bu çalışma, bıldırcın yemlerine aflatoksin (AF) (60 μg/kg yem) ilavesiyle deneysel olarak oluşturulan kronik aflatoksikoziste, içme suyuna 200 mg/l dozda L-karnitin verilmesinin etkilerinin araştırılması amacıyla yapıldı. Araştırmada 8 haftalık yaşta 80 adet bıldırcın kullanıldı. Hayvanlar dört eşit gruba (n=20) ayrıldı. Grup II’teki hayvanlara 200 mg/l içme suyu dozunda L-karnitin (Carbitol, DİF-İstanbul), grup III’teki hayvanlara 60 μg/kg yem dozunda AF yeme katılarak ve grup IV’teki hayvanlara 60 μg/kg yem dozunda AF ile 200 mg/l içme suyu dozunda Lkarnitin oral olarak 60 gün boyunca verildi. Yemlere düşük düzeyde AF (60 μg/kg yem) ilavesinin toplam bilirubin düzeyleri (p

Anahtar Kelimeler:

biyokimyasal parametreler, Japon bıldırcını, aflatoksin, karnitin, hematoloji, patoloji, kümes hayvanları beslenmesi, toksisite, vitamin b kompleksi

Effect of L-Carnitine supplementation on biochemical, Haematological and pathological parameters of quails (Coturnix coturnix japonica) during chronic aflatoxicosis

The aim of this study was to evaluate the protective efficacy of L-carnitine administration in the prevention of toxic effects of experimental chronic aflatoxicosis. A total of 80 and eight weeks old Japanese quail (Coturnix coturnix japonica) were used in this experiment. They were divided into one control and three treatment group each containing 20 quails 􀀀group II L-carnitine 200 mg/l via drinking water, group III aflatoxin (AF) 60 μg/kg feed, group IV AF (60 μg/kg feed) plus L-carnitine (200 mg/l via drinking water)􀀀. The experimental period lasted 60 days. Control group was fed with unsupplemented basal diet and water. The low dose of AF (60 μg/kg feed) significantly increased the level of total bilirubin (p<0.001) and AST enzyme activity (p<0.001); and significantly decreased the levels of glucose (p<0.05), total cholesterol (p<0.05), VLDL (p<0.001), triglyceride (p<0.001), albumin (p<0.001) and calcium (p<0.001). The low dose of AF (60 μg/kg feed) significantly decreased the count of PCV (p<0.01) and RBC (p<0.001) and significantly prolonged the coagulation time (p<0.001) when compared with control group. AST enzyme activity and bilirubin concentrations statistically decreased in AF+L-carnitine (group IV) when compared to AF (group III) (p<0.001) while glucose (p<0.05), albumin (p<0.001) haemotocrit (p<0.01) and RBC (p<0.001) increased and coagulation time shortened (p<0.001). L-carnitine supplementation was shown to partially protect on glucose, albumin, bilirubin concentrations, RBC and coagulation time and to fully protect haemotocrit level and AST enzyme activity against adverse effect of AF. It is concluded that the supplementation of the low dose AF (60 μg/kg feed) had significantly alterations and toxic effects on pathological, biochemical and haematological parameters and L-carnitine administration (200 mg/l via drinking water) had partial protective effect against the toxic effects of AF on pathological, biochemical and haematological parameters of quails (Coturnix coturnix japonica) during experimental chronic aflatoxicosis.

Keywords:

biochemical parameters, Japanese quails, aflatoxins, carnitine, haematology, pathology, poultry feeding, toxicity, vitamin B complex,

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Giambrone JJ, Diener UL, Davis ND, Panangala VS, Hoerr FJ: Effects of aflatoxin on young turkeys and broilers chickens. Poult Sci, 64, 1678–1684, 1985.
Moss MO: Mycotoxic fungi. In, Eley AR (Ed): Microbial food poisoning. Second ed. Chapmen and Hall, USA, 1996.
Bailey RH, Kubena LF, Harvey RB, Buckley SA, Rottinghaus GE: Efficacy of various inorganic sorbents to reduce the toxicity of aflatoxin and T-2 toxin in broiler chickens. Poult Sci, 77, 1623–1630, 1998.
Kubena LF, Harvey RB, Bailey RH, Buckley SA, Rottinghaus GE: Effects of hydrated sodium calcium aluminosilicate (TBind) on mycotoxicosis in young broiler chickens. Poult Sci, 77, 1502–1509, 1998.
Santurio JM, Mallmann CA, Rosa AP, Appel G, Heer A, Dageforde S, Bottcher M: Effect of sodium bentonite on the performance and blood variables of broiler chickens intoxicated with aflatoxin. Br Poult Sci, 40, 115–119, 1999.
Oğuz H, Kurtoğlu V: Effect of clinoptilolite on performance of broiler chickens during experimental aflatoxicosis Br Poult Sci, 4,: 512–517, 2000.
Huff WE, Harvey RB, Kubena LF, Rottinghaus GE: Toxic synergism between aflatoxin and T-2 toxin in broiler chicken. Poult Sci, 6, 1418–1420, 1988.
Keçeci T. Oğuz H, Kurtoğlu V, Demet Ö: Effects of polyvinylpolypyrrolidone, synthetic zeolite and bentonite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis, Br Poult Sci, 39, 452–458, 1998.
Sengstag C, Weibel B, Fasullo M: Genotoxicity of aflatoxin B1: Evidence for a recombinant-mediated mechanisms in Saccharomyces cerevisiae. Cancer Res, 56, 5457-5465, 1996.
Çelik I, Oğuz H, Demet Ö, Boydak M, Dönmez HH, Sur E, Nizamlıoğlu F: Embryotoxicity assay of aflatoxin produced by Aspergillus parasiticus NRRL 2999. Br Poult Sci, 41, 401-409, 2000.
Parlat SS, Yıldız AÖ, Oğuz H: Effect of clinoptilolite on performance of Japanese quail (Coturnix coturnix japonica) during experimental aflatoxicosis Br Poult Sci, 40, 495–500, 1999.
Ibrahim IK, Shareef AM, Al-Joubory KMT: Ameliorative effects of sodium bentonite on phagocytosis and Newcastle disease antibody formation in broiler chickens during aflatoxicosis. Res Vet Sci, 69, 119–122, 2000.
Oğuz H, Hadimli HH, Kurtoğlu V, Erganiş O: Evaluation of humoral immunity of broiler during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Revue de Med Vet, 154, 483-486, 2003.
Kıran MM, Demet Ö, Ortatatlı M, Oğuz H: The preventive effect of polyvinylpolypyrrolidone on aflatoxicosis in broilers. Avian Pathol, 27, 250–255, 1998.
Ledoux DR, Rottinghaus GE, Bermudez AJ, Alanso-Debolt M: Efficacy of hydrated sodium calcium aluminosilicate to ameliorate the toxic effects of aflatoxin in broiler chicks. Poult Sci, 78, 204–210, 1999.
Çelik I, Demet Ö, Dönmez HH, Oğuz H, Boydak M: Determination of phagocytic and candidacidal activities of peritoneal macrophages isolated from chickens fed with aflatoxin and an aflatoxin adsorbing agent, polyvinylpolypyrrolidone. Vet Bil Derg, 12, 145–151, 1996.
Gabal MA, Azzam AH: Interaction of aflatoxin in the feed and immunisation against selected infectious diseases in poultry. II. Effect on one-day-old layer chicks simultaneously vaccinated against Newcastle disease infectious bronchitis and infectious bursal disease. Avian Pathol, 27, 290–295, 1998.
Ibrahim IK, Al-Joubory KMT, Shareef AM: Reducing aflatoxicosis in growing chicks by dietary sodium bentonite. IPA J Agri Res, 69, 130-138, 1998.
Oğuz H, Keçeci T, Birdane YO, Önder F, Kurtoğlu V: Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during experimental aflatoxicosis. Res Vet Sci, 69, 89–93, 2000.
Rosa CA, Miazzo R, Magnoli C, Salvano M, Chiac SM, Ferrero S, Saenz M, Carvalho EC, Dalcero A: Evaluation of the efficacy of bentonite from the south of Argentina to ameliorate the toxic effects of AF in broilers. Poult Sci, 80, 139–144, 2001.
Harvey RB, Kubena LF, Ellisalde MH, Phillips TD: Efficacy of zeolitic ore compounds on the toxicity of aflatoxin to growing broiler chickens. Avian Dis, 37, 67–73, 1993.
Fernandez A, Verde M, Gascon M, Ramos J, Gomez J, Luco DF, Chavez G: Variations of clinical, biochemical parameters of laying hens and broiler chickens fed aflatoxin-containing feed. Avian Pathology, 23, 37–47, 1994.
Amer AMM, Fahim EMM, Ibrahim RK: Effect of aflatoxicosis on the kinetic behaviour of ceftiofur in chickens. Res Vet Sci, 65, 115–118, 1998.
Bremer J: Carnitine metabolism and functions, Physiol Rev, 63, 1420-80, 1983.
Rebouche CJ, H Seim: Carnitine metabolism and its regulation in microorganisms and mammals. Annu Rev Nutr, 18: 39-61, 1998.
Fritz IB, Arrigoni-Martelli E: Sites of action of carnitine and its derivatives on the cardiovascular system. Interactions with membranes, Trend Pharmacol Sci, 14, 355-60, 1993.
O’Connor CE, Costel M, Grisolia S: Protective effect of L-Carnitine on hiperammonemia. FEBS Lett, 166, 331-334, 1984.
Tremblay GC, Bradley TM: L-Carnitine protects fish against acute ammonia toxicity. Comp Biochem Physiol, 101, 349- 351, 1992.
Çitil M, Güneş V, Atakişi O, Özcan A, Tuzcu M, Doğan A: Protective Effect of L-Carnitine against oxidative damage caused by experimental chronic aflatoxicosis in quail (Coturnix coturnix). Acta Vet Hung, 53(3): 319-324, 2005.
Kart A, Yapar K, Karapehlivan M, Tunca R, Öğün M, Çitil M: Effects of L-carnitine on kidney histopathology, plasma and tissue total sialic acid, malondialdehyde and glutathione concentrations in response to gentamicin administration in Balb/C Mice. Revue de Med Vet, 157(4): 179-187, 2006.
Kart A, Yapar K, Karapehlivan M, Çitil M: The possible protective effect of L-carnitine on tilmicosin-induced cardiotoxicity in mice. J Vet Med-A, 54(3): 144-146, 2006.
Abd-Allah ARA: L-Carnitine ameliorates immunologicalinduced hepatitis in rats. Saudi Pharmaceut J, 14(1): 59-68, 2006.
Atroshi F., Rizzo A, Westermarck T, Ali-Vehmas T: Effects of tamoxifen, melatonin, coenzyme Q10, and L-carnitine supplementation on bacterial growth in the presence of mycotoxins. Pharmacol Res, 38(4): 289-295, 1998.
Sachan DS, Yatim AM: Suppression of aflatoxin B1-induced lipid abnormalities and macromolecule-adduct formation by L-carnitine. J Environ Pathol Toxicol Oncol, 11(4): 205-210, 1992.
Yatim AM, Sachan DS: Carnitine alters binding of aflatoxin to DNA and proteins in rat hepatocytes and cell-free systems. J Nutr, 131, 1903–1908, 2001.
Ortatatlı M, Oğuz H: Ameliorative effects of dietary clinoptilolite on pathological changes in broiler chickens during aflatoxicosis. Res Vet Sci, 71, 59-66, 2001.
NRC: Nutrient Requirement of Poultry, Washington, D.C., 1984. Shotwell OL, Hesseltine CV, Stubblefield RD, Sorenson WG: Production of aflatoxin on rice. Appl Microbiol, 14, 425–429, 1966.
Oğuz H: Broiler yemlerinde aflatoksikozise karşı polyvinylpolypyrrolidone’nun (PVPP) tek başına veya diğer absorbanlarla kombinasyonunun koruyucu etkileri. Doktora tezi, Selçuk Üniversitesi, Sağlık Bilimler Enstitüsü, Konya, 1997.
Konuk T: Pratik Fizyoloji I. , II. Baskı, AÜ Basımevi, Ankara, 1981.
Scheideler SE: Effects of various types aluminosilicates and aflatoxin B1 on aflatoxin toxicity, chick performance, and mineral status. Poult Sci, 72, 282–288, 1993.
Huff WE, Kubena LF, Harvey RB, Phillips TD: Efficacy of hydrated sodium calcium aluminosilicate to reduce the individual and combined toxicity of aflatoxin and ochratoxin A. Poult Sci, 71, 64–69, 1992.
Andrieu-Abadie N, Jaffrezou J, Levade T, Mercadier JJ: Lcarnitine prevents doxorubicin-induced apoptosis of cardiac myocytes: role of inhibition of ceramide generation. FASEB J, 13(12): 1501-1510, 1999.
Miguez MP, Soler F, Garcia-Rubio L: Accentuation of paraquat-induced toxicity by L-carnitine in mice. Biofactors, 8(1-2): 73-78, 1998.
Kashiwagi A, Kanno T, Arita K, Ishisaka R, Utsumi T, Utsumi K: Suppression of T(3)- and fatty acid-induced membrane permeability transition by L-carnitine. Comp Biochem Physiol Biochem Mol Biol, 130, 411- 418, 2001.
Donaldson WE, Tung HT, Hamilton PB: Depression of fatty acid synthesis in chick (Gallus domesticus) liver by aflatoxin. Comp Biochem Physiol, 41, 843–847, 1972.
Tung HT, Donaldson WE, Hamilton PB: Altered lipid transport during aflatoxicosis. Toxicol Appl Pharmacol, 22, 97–104, 1972.
Kato R, Onoda K, Omori Y: Effect of aflatoxin B1 on the incorporation of 14 C-acetate into cholesterol by rat liver. Exper, 25, 1026, 1969.
Basmacıoğlu H, Oğuz H, Ergül M, Çöl R, Birdane YO: Effect of dietary esterified glucomannan on performance, serum biochemistry and haematology in broilers exposed to aflatoxin. Czech J Anim Sci, 50, 31—39, 2005.
Gelbolin HV, Wortham JS, Wilson RG, Freidman M, Wogan GN: Rapid and marked inhibition of ratliver RNA polymerase by aflatoxin B1. Sci, 154, 1205–1206, 1966.
Yu S: Mechanism of aflatoxin B1 inhibition of rat hepatic nuclear RNA synthesis. J Biol Chem, 252, 3245–3251, 1977.
Dirr HW, Schabort JC: Aflatoxin B1 transport in rat blood plasma. Binding to albumin in vivo and in vitro and spectrofluorimetric studies into the nature of the interaction. Biochem Biophys Acta, 881, 383–390, 1986.
Glahn RP, Beers KW, Bottje WG, Wideman RF, Huff WE: Research note: Altered renal function in broilers during aflatoxicosis. Poult Sci, 69, 1796–1799, 1990.
Glahn RP, Beers KW, Bottje WG, Wideman RF, Huff WE, Thomas W: Aflatoxicosis alters avian renal function, calcium, and vitamin D metabolism. J Toxicol Environ Health, 34, 309–321, 1991.
Bertelli A, Conte A, Ronca G: L-propionyl carnitine protects erythrocytes and low density lipoproteins against peroxidation. Drugs Exp Clin Res, 20, 191-197, 1994.
Nikolaos S, George A, Telemachos T, Maria S, Yannis M, Konstantinos M: Effect of L-carnitine supplementation on red blood cells deformability in hemodialysis patients. Ren Fail, 22, 73-80, 2000.
Matsumoto Y, Amano I, Hirose S, Tsuruta Y, Hara S, Murata M, Imai T: Effects of L-carnitine supplementation on renal anemia in poor responders to erythropoietin. Blood Purif, 19, 24-32, 2001.
Fernandez A, Verde MT, Gomez J, Gascon M, Ramos JJ: Changes in prothrombin time, haematology and serum proteins during experimental aflatoxicosis in hens and broiler chickens. Res Vet Sci, 58, 119-122, 1995.
Karaman, M., Basmacıoğlu H., Ortatatlı M., Oğuz H: Evaluation of the detoxifying effect of yeast glucomannan on aflatoxicosis in broilers as assessed by gross examination and histopathology. Br Poult Sci, 46(3): 394–400, 2005.
Ortatatlı M, Oğuz H, Hatipoğlu F, Karaman M: Evaluation of pathological changes in broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Res Vet Sci, 78, 61–68, 2005.