Taylan Özgür KAYA, Süleyman GÜL, GÖKHAN NUR

Karyotype analysis in orthrias angorae (Steindachner, 1897)

Orthrias angorae (Steindachner, 1897) (Fam: Balitoridae)'mn kromozomlarının sayı ve yapıları incelenerek, karyotip analizi yapılmıştır. Bu çalışmada kullanılan balıklar Kura-Aras havzasından ağlarla yakalanarak laboratuvara getirilmiştir. Her bir gram vücut ağırlığı için 0.01 mi, %0.6'hk kolsişin solüsyonu balıkların karın boşluğuna enjekte edilmiş ve balık kesilmeden önce 190 dakika beklenilmiştir. Metafaz incelemeleri ile O. angorae'nm 2n=50 kromozoma sahip olduğu belirlenmiştir. Karyotiplerinin; 7 metasentrik, 7 submetasentrik ve 11 akrosentrik kromozom çiftinden (NF=78) oluştuğu tespit edilmiştir. Bu türde cinsiyete bağlı herhangi bir kromozom tespit edilememiştir.

Anahtar Kelimeler:

metafaz, kromozomlar, Türkiye, genetik analizler, eşeysel farklılık, balık, karyotip

Çöpçü Balığı (Orthrias angorae, Staindachner 1897)' nda karyotip analizi

Karyotype analysis was performed in Orthrias angorae (Steindachner, 1897) (Fam: Balitoridae) by investigating the number and structures of their chromosomes. The fish used in this study were caught with fishing nets from the Kura-Aras river basin and taken to the laboratory. Fishes were injected intraperitoneally (i.p.) with doses of 0.01 ml/g body weight of 0.6% solution of colchicine and left for 190 minutes before sacrification. It was determined that O. angorae had 2n=50 chromosomes by metaphase investigation. Their karyotypes were determined as being composed of 7 metacentric, 7 submetacentric and 11 acrocentric chromosome pairs with NF:78. We were unable to identify any sex-related chromosomes in this species.

Keywords:

metaphase, chromosomes, Turkey, genetic analysis, sexual dimorphism, fishes, karyotypes,

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