Isolation of Rhodococcus equi from a horse with corneal ulcer

Sağ gözü keratitis ülserosalı olan bir attan Rhodococcu equi izole ve identifiye edildi. Numunenin direkt mikroskobik incelemesi yapıldı. Bakterinin izolasyonu amacıyla kültürel yöntemlerden yararlanıldı. Kültürel yoklama sonucu keratitis ülserosalı bölgeden R. equi izole edildi. Antibiyotik duyarlılık testinde Kirby-Bauer disk diffüzyon metodu kullanıldı. Bu yönteme göre izole edilen bakteri, eritromycin, rifampycin, vankomycin, azithromycin, ciprofloxacin, clindamicin, gentamicine duyarlı ve penicilline orta derecede duyarlı bulundu. Atların keratitis olgularından nonpesifik ve spesifik bakteriler izole edilmesine rağmen, böyle olgulardan R. equi izolasyonu çok seyrek olarak bildirilmiştir. Böyle bir olgudan hastanın 9 yaşında oluşu kadar infeksiyon etkeni olarak R. equi izole edilmiş olması da ilginç bir bulgu olarak değerlendirildi.

Korneal ülserli bir attan Rhodococcus iqui izolasyonu

R.equi isolated and identificated in a horse with keratitis ulcerosa. Direct microscobic examination and cultural methods were used to isolate the bacteria. The Kirby-Bauer disc diffusion method was used for antibiotic sensitivity test. Rhodococcus equi was isolated from the horse with keratitis ulcerosa. The isolate was sensitive to eritromycin, rifampycin, vankomycin, azithromycin, ciprofloxacin, clindamicin, gentamicin,but intermediate sensitive to penicillin. Although, various nonspecific and specific bacteria are commonly reported in equine keratitis, however this is the report describing the involvement of R. equi isolation has been reported rarely in such a case.

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  • 1.Hillidge CJ: Review of Corynebacterium (Rhodococcus) equi lung abscesses in foals: pathogenesis, diagnosis and treatment. Veterinary Record ,13 : 261-263,1986.
  • 2.Karadaş E, Gülcü HB, Beytut E, Kahraman MM: İki arap tayında Rhodococcus (Corynebacterium) equi enfeksiyonu. Fırat Üniversitesi Sağlık Bilimleri Dergisi, 10(2); 321-325,1996.
  • 3.Ishino S, Kumagai K, Kuniyoshi S, Nakazawa M, Matsuda I, Oka M: Immunohistochemical observations on pneumonic lesions caused by Rhodococcus equi in foals. Journal of Veterinary Medicine Science, 54(3): 509-515,1992.
  • 4.Falcon J, Smith BP, O'Brien TR, Carlson GP, Biberstein E: Clinical and radiographic findings in Corynebacterium equi pneumonia of foals. Journal of the American Veterinary Medical Association, 186(6): 593- 599, 1985.
  • 5.Ardans AA, Hietala SK, Spensley MS, Sansome A: Studies of naturally occurring and experimental Rhodococcus equi pneumonia in foals. Proceedings of the American Association of Equine Practitioner, 32: 129- 144,1987.
  • 6.Johnson JA, Prescott JF, Markham JF: The pathology of experimental Corynebacterium equi infections in foals following intragastric challenge. Veterinary Pathology, 20:450-459, 1983.
  • 7.Zink CM, Yager JA, Smart NL: Corynebacterium equi infections in horses, 1958-1984: A Review of 131 Cases. Canadian Veterinary Journal, 27:213-217, 1986.
  • 8.Quinn PJ, Carter ME, Markey BK, Carter GR: Clinical Veterinary Microbiology. London, Mosby-Year Book. England, 137-143, 1998.
  • 9.Bauer AN, Kirby WMM, Sherris JC, Turek M: Antibiotic susceptibility testing by a standardized single disc method. Journal of Clinical Pathology, 45:493-494, 1966.
  • 10.Nasser AA, Bizri AR: Chronic scalp wound infection due to Rhodococcus equi in an immunocompetent patient. Journal of Infection, 42( 1 ):67-68, 2001.
  • 11.Gorham S, Haaland MA: The effects of a disseminated Corynebacterium equi infection on a neonatal foal. Veterinary Medicine, 552-554,1986.
  • 12.Davis WP, Steificek BA, Watson GL, Yamini B, Madarame H, Takai S, Render SA: Disseminated Rhodococcus equi infection in two goats. Veterinary Pathology, 36(4):336-339, 1999.
  • 13.Brooks DE, Andrew SE, Biros DJ, Denis HM, Cutler TJ, Strubbe DT, Gelatt KN: Ulcerative keratitis caused by beta hemolytic Streptococcus equi in 11 horses. Veterinary Ophthalmology, 3(2-3): 121 -125, 2000.
  • 14.Moore CP, Heller N, Majors LJ, Whitley RD, Burgess EC, Weber J: Prevalence of ocular microorganisms in hospitalized and stabled horses. American Journal of Veterinary Research, 49(6):773-777, 1988.
  • 15.Gelatt KN: Pseudomonas ulcerative keratitis and abscess in a horse. Veterinary Medicine - Small Animal Clinican, 69 (10): 1309-1310,1974.
  • 16.Sanchez S, StuderM, CurrinP, BarlettP, DounnousD: Listeria keratitis in a horse. Veterinary Ophthalmology, 4(3): 217-219,2001.