MUSTAFA KIZILŞİMŞEK, MUSTAFA KÜSEK, YEKTA GEZGİNÇ, ADEM EROL

Isolation and Identification of High Lactic Acid Producer Bacteria from Forage and Their Silages Grown in Different Ecologies

Türkiye'nin geniş bir bölümündeki meralarda bulunan yem bitkilerinden ve bunların silajlarından 695 adet LAB izolasyonu yapılmıştır. Elde edilen izolatlardan önemli bir çoğunluğu (531 adet) rejenere olabilmiştir. Bu izolatlar CaCO3 içeren MRS agar besi yerinde inkübe edilmiş ve toplam organik asit üretimleri belirlenmiştir. Toplam asit üretimi yüksek olan 70 adet izolat MRS broth besi yerinde inkübe edilerek, laktik asit üretimleri belirlenmiştir. Bu izolatlar içerisinden laktik asit üretimi yüksek olduğu tespit edilen 10 adet izolat seçilmiştir. Seçilen 10 adet izolatın BIOLOG cihazında inkübasyon süresi boyunca farklı karbonhidrat kaynaklarını kullanma esasına göre tanımlaması yapılmıştır.

Farklı Ekolojilerdeki Yem Bitkilerinden ve Silajlarından Yüksek Laktik Asit Üreten Bakteri İzolasyonu ve Tanımlanması

In total, 695 Lactic acid bacteria isolation was made from forage crops grown in a wide part of Turkey's rangeland flora and their silages. A big majority of isolated strains (531) could regenerate. All regenerated isolates were incubated in MRS agar media containing CaCO3 in order to determine their total organic acid production. Selected 70 isolates according to their organic acid production were incubated in MRS broth media and their lactic acid productions were determined. High lactic acid producer 10 isolates were selected among treated isolates and they were identified using BIOLOG device in terms of their individual usage of different carbohydrate source during incubation period.

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Kizilsimsek M, Schmidtt RJ, Kung L Jr: Effects of a mixture of lactic acid bacteria applied as a freeze-dried or fresh culture on the fermentation of alfalfa silage. J Dairy Sci. 90, 5698-5705, 2007. DOI: 10.3168/jds. 0448
Durmaz H, Avcı M, Aygün O: The precence of Listeria species in corn silage and raw milk produced in southeast region of Turkey. Kafkas Univ Vet Fak Derg, 21, 41-44 2015. DOI: 10.9775/kvfd.2014.11664
Van Soest PJ, Robertson JD, Lewis BA: Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci. 74, 3583-3597, 1991. DOI: 10.3168/jds.S0022- (91)78551-2
Kung L Jr, Tung S, Maciorowski KG, Buffum K, Knutsen K, Aimutus WR: Effect of plant cell wall degrading enzymes and lactic acid bacteria on silage fermentation and composition. J Dairy Sci, 74, 4284-4296, 1991. DOI: 10.3168/jds.S0022-0302(91)78623-2
Stokes M R: Effects of an enzymes mixture, an inoculant and their interaction on silage fermentation and dairy production. J Dairy Sci. :764-773, 1992. DOI:10.3168/jds.S0022-0302(92)77814-X
Pitt RE: The probability of inoculant effectiveness in alfalfa silages. ASAE, 33,1771-1778, 1990. DOI: 10.13031/2013.31538
Jones JB, Chase AR, Haris GK: Evaluation of the biolog GN microplate system for identification of some plant pathogenic bacteria. Plant Disease, , 553-558, 1993. DOI: 10.1094/PD-77-0553
Hammes WP, Vogel RF: The genus Lactobacillus. In, Wood BJB, Holzapfel WH (Eds): In the Genera of Lactic Acid Bacteria. 19-54, Chapman & Hall, London, 1995.
Hammes WP, Hertel C: Genus Lactobacillus. In, De Vos P. Garrity GM, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH, Whitman WB: Bergey's Manual of Systematic Bacteriology. 2nd ed., 465-511, Springer, NewYork, USA, 2009.
Pahlow G, Muck RE, Driehuis F, Oude Elferink SJW, Spoelstra A: Microbiology of ensiling. In, Buxon DR, Muck RE, Harrison JH (Eds): Silage Science and Technology. 31-93, ISBN: 0-89118-151-2, Springer, NewYork, USA, 2003.