Seçil ABAY, FUAT AYDIN, HARUN HIZLISOY, VEHBİ GÜNEŞ

Recovery of thermophilic Campylobacter spp. in healthy and diarrhoeic pets by three culture methods and identification of the isolates by multiplex polymerase chain reaction (mPCR)

Bu çalışmada, sağlıklı ve ishalli kedi ve köpeklerde 3 farklı kültür metodu kullanılarak termofilik Campylobacter türlerinin prevalansının belirlenmesi amaçlandı. Bu amaçla, 120 köpek, 15 kediden rektal svap örneği toplandı ve bunların 14ü (12 köpek ve 2 kedi) ishalli hayvanlardan alındı. Termofilik Campylobacter spp.nin izolasyonunda, tüm örnekler için CCDA (cefoperazone, amphotericin B) ya da CAT suplement (cefoperazone, amphotericin and teicoplanin) ilave edilmiş modified charcoal cefoperazone deoxycholate agar (mCCDA)a (sefoperazon, amfoterisin B) direkt ekim, ishalli hayvanlardan alınan örnekler için de %5 defibrine koyun kanı ilave edilmiş Mueller- Hinton Agar üzerine membran fltrasyon yöntemi kullanıldı. İzolatların identifikasyonu multipleks polimeraz zincir reaksiyonu (mPZR) ile gerçekleştirildi. Campylobacter türlerinin köpek ve kedilerde genel prevalansı sırasıyla %40.0 ve %26.7 olarak bulundu. Campylobacter jejuni en sık rastlanan tür olup 36 köpek ve 4 kediden izole edildi. Ayrıca, 10 köpek ve 1 kediden C. upsaliensis, 5 köpekten C. coli ve 2 sağlıklı köpekten C. lari izole edildi. Termofilik Campylobacter spp. izolasyonu için CAT selektif suplementin kullanıldığı metot köpeklerde daha duyarlı iken ishalli köpeklerde membran filtrasyonun en uygun metot olduğu görüldü. Bu sonuçlar, özellikle sağlıklı köpeklerde oldukça yüksek oranlarda bulunan Campylobacter spp. taşıyıcılığının halk sağlığı için göz ardı edilemez risk oluşturduğunu göstermektedir.

Sağlıklı ve ishalli pet hayvanlarda Termofilik Campylobacter spp.nin üç kültür metodu ile izolasyonu ve izolatların multipleks polimeraz zincir reaksiyonu m(PZR) ile identifikasyonu

In this study, the determination of prevalence of thermophilic Campylobacter species in dogs and cats with and without diarrhoea using 3 diferent cultural methods was aimed. For this purpose, rectal swabs were collected from 120 dogs and 15 cats and 14 of them (12 dogs and 2 cats) were taken from diarrhoeic animals. The isolation of thermophilic Campylobacter spp. was conducted by direct plating onto modified charcoal cefoperazone deoxycholate agar (mCCDA) supplemented with CCDA (cefoperazone, amphotericin B) or CAT (cefoperazone, amphotericin and teicoplanin) for all samples and membrane filtration method onto Mueller-Hinton Agar supplemented with 5% defibrinated sheep blood for samples from diarrhoeic pets and identification of isolates was performed using multiplex polymerase chain reaction (mPCR). The overall prevalence of Campylobacter species was found to be 40.0% and 26.7% in dogs and cats, respectively. Campylobacter jejuni was the most frequent bacterium isolated from 36 dogs and 4 cats. C. upsaliensis, C. coli and C. lari were isolated from 10 dogs and 1 cat, 5 dogs and 2 healthy dogs respectively. For the isolation of thermophilic Campylobacter spp., whilst the method using CAT as selective supplement being more sensitive in dogs, the membrane filtration appeared as the most suitable method in diarrhoeic dogs. These results showed the occurrence of a relatively high carriage of Campylobacter spp., particularly in healthy dogs that may constitute a non negligible risk for public health.

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