TAKİ KARSLI, Emine ŞAHİN, Bahar Argun KARSLI, SEZAİ ALKAN, MURAT SONER BALCIOĞLU

Identification of Alleles for Factor XI (FXID) and Uridine Monophosphate Synthase (DUMPS) deficiencies in Holstein cows reared in Antalya

Faktör XI (FXID) ve Üridin Monofosfat Senteaz Eksiklikleri (DUMPS) Siyah Alaca sığır ırkında otozomal resesif kalıtım gösteren kalıtsal hastalıklard ır. Bu çalışmanın amacı Antalya bölgesinde yetiştirilen Siyah Alaca ırkı sığırlarda DUMPS ve FXI eksikliği allelinin bulunup bulunmadığının araştırılmasıdır. Bu çalışmada 504 Siyah Alaca inek kullanılmıştır. FXI genotiplerinin belirlenmesi için PCR (Polimeraz Zincir Reaksiyonu), DUMPS genotiplerinin belirlenmesi için PCR-RFLP (PCR- Restriksiyon Parça Uzunluk Polimorfi zmi) yöntemi kullanılmıştır. Sonuç olarak DUMPS alleli tespit edilemezken FXI taşıyıcılarının oranı %0.4 olarak bulunmuştur.

Anahtar Kelimeler:

sığır, Alacalı Sığır, Türkiye, alleller, inek, süt sığırı, süt ineği, genler, genotip, tanı, Antalya

Antalya’da yetiştirilen Siyah Alaca İneklerde Faktör XI (FXID) ve Üridin Monofosfat Senteaz eksikliği (DUMPS) allellerinin belirlenmesi

Defi ciencies of Factor XI (FXID) and Uridine Monophosphate Synthase (DUMPS) are autosomal recessive inherited diseases in Holstein cattle breed. The aim of this study was to investigate whether FXI defi ciency and DUMPS alleles exist in Holstein cattle reared in Antalya region Turkey or not. 504 Holstein cows were used for this study. PCR (Polymerase Chain Reaction) was used for determination of FXI genotypes and, PCR-RFLP (PCR- Restriction Fragment Length Polymorphism) was used for determination of DUMPS genotypes. As a result, DUMPS allele was not detected while the prevalence of FXID carriers was found as 0.4%.

Keywords:

cattle, Holstein-Friesian, Turkey, alleles, cows, dairy cattle, dairy cows, genes, genotypes, identification, Antalya,

PDF

___

1. Marron BM, Robinson JL, Gentry PA, Beever JE: Identification of a mutation associated with factor XI deficiency in Holstein cattle. Anim Genet, 35, 454-456, 2004.
2. Brush PJ, Anderson PH, Gunning RF: Identification of factor XI deficiency in Holstein Friesian cattle in Britain. Vet Rec, 121, 14-17, 1987.
3. Shanks RD, Robinson JL: Embryonic mortality attributed to inherited deficiency of uridine monophosphate synthase. J Dairy Sci, 72, 3035-3039, 1989.
4. Robinson JL, Popp RG, Shanks RD, Oosterhof A, Veerkamp JH: Testing for deficiency of uridine monophosphate synthase among Holstein-Frisian cat tle of North America and Europe. Livest Prod Sci, 36, 287-298, 1993.
5. Citek J, Blahova B: Recessive disorders-a serious health hazard? J Appl Biomed, 2, 187-194, 2004.
6. Akyüz B, Ertuğrul O, Ağaoğlu KÖ: Detection of Bovine Leukocyte Adhesion Deficiency (BLAD) allele in Holstein cows reared in Kayseri vicinity. Kafkas Univ Vet Fak Derg, 16 (2): 519-521, 2010.
7. Schwenger B, Tammen I, Aurich C: Detection of homozygous recessive genotype for deficiency of uridine monophosphate synthase by DNA typing among bovine embryos produced in vitro. J Reprod Fertil, 100, 511-514, 1994.
8. Meydan H, Yıldız MA, Agerholm JS: Screening for bovine leukocyte adhesion deficiency, deficiency of uridine monophosphate synthase, complex vertebral malformation, bovine citrullinaemia, and factor XI deficiency in Holstein cows reared in Turkey. Acta Vet Scan, 52 (56): 1-8, 2010.
9. Akyüz B, Kul BÇ: Detection of deficiency of uridine monophosphate synthase (DUMPS) in female holstein cattle in Turkey. Ankara Univ Vet Fak Derg, 56, 231-232, 2009.
10. Öner Y, Keskin A, Elmacı C: Identification of BLAD, DUMP, citrullinamia and factor XI defiency in Holstein cattle in Turkey. Asian J Anim Vet Adv, 5 (1): 60-65, 2010.
11. Kaminski S, Grzybowski G, Prusak B, Rusc A: No incidence of DUMPS carriers in Polish dairy cattle. J Appl Genet, 46 (4): 395-397, 2005.
12. Patel RK, Krishna MS, Kalpesh JS, Jenabhai BC, Krothapalli RS, Sambasiva R: Lack of carriers of citrullinaemia and DUMPS in Indian Holstein cattle. J Appl Genet, 47, 239-242, 2006.
13. Rahimi G, Nejati-Javaremi A, Olek K: Genotyping BLAD, DUMPS and -CSN loci in Holstein young bulls of the National Animal Breeding Center of Iran. Pakistan J Biol Sci, 7, 1389-1392, 2006.
14. Citek J, Rehout V, Hanusova L, Vrabcova P: Sporadic incidence of factor XI deficiency in Holstein cattle. J Sci Food Agric, 88, 2069-2072, 2008.
15. Meydan H, Yıldız MA, Özdil F, Gedik Y, Özbeyaz C: Identification of factor XI deficiency in Holstein cattle in Turkey. Acta Vet Scan, 51 (5): 1-4, 2009.