Yasemin ÖNER, Murat PULLU, Oya AKIN, CENGİZ ELMACI

Bursa bölgesinde yetiştirilen İsviçre Esmeri ve Siyah Alaca ırkı sığırlarda beta laktoglobulin (β-lg) ve büyüme hormonu (bGH) gen polimorfizmlerinin HaeIII ve MspI restriksiyon enzimleri kullanılarak incelenmesi

Bu çalışmada Bursa bölgesinde yetiştirilen İsviçre Esmeri ve Siyah Alaca sığırlarda β-lg ve bGH gen polimorfi zmleri HaeIII ve MspI restriksiyon enzimleri kullanılarak incelenmiştir. İsviçre Esmer’lerinde β-lg A ve B allellerinin frekansları sırasıyla 0.3430 ve 0.6570 olarak bulunurken, Siyah Alaca ırkına ait örneklerde sırasıyla 0.5480 ve 0.4520 olarak saptanmıştır. Hem İsviçre Esmeri hem de Siyah Alaca ırklarına ait örneklerde bGH’nun MspI (+) allellinin predominat olduğu belirlenmiştir. İsviçre Esmerlerinde MspI (+) allellinin frekansı 0.9623, Siyah Alacalarda ise 0.8158 olarak hesaplanmıştır. Her iki populasyon, her iki lokus bakımdan Hardy-Weinberg dengesinde bulunmuştur.

Anahtar Kelimeler:

genetik polimorfizm, populasyon genetiği, genetik analizler, somatotropin, Bursa (şehir), genler, süt ineği, süt sığırı, inek, sığır, beta-laktoglobulin, alleller, Türkiye, Alacalı Sığır, Amerikan Esmer Irkı

Investigation of Beta-lactoglobulin (β-lg) and bovine growth hormone (bGH) genes polymorphisms By using HaeIII and MspI Restriction Enzymes in Brown Swiss and Holstein Breeds reared in Bursa region

In this study polymorphism on β-lg and bGH genes in Holstein and Brown Swiss cattle reared in Bursa region were investigated by using HaeIII and MspI restriction enzymes. While frequencies of A and B allelles of β-lg were found 0.3430 and 0.6570 in Brown Swiss population, they were found as 0.5480 and 0.4520 in Holstein population, respectively. MspI (+) allelle of bGH was predominat in both Brown Swiss and Holstein populations with frequency of 0.9623 and 0.8158, respectively. Both of two populations were found in Hardy- Weinberg equilibrium for both of the two loci investigated.

Keywords:

genetic polymorphism, population genetics, genetic analysis, somatotropin, Bursa, genes, dairy cows, dairy cattle, cows, cattle, beta-lactoglobulin, alleles, Turkey, Holstein-Friesian, American Brown Swiss,

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