Growth hormone gene polymorphism in four cattle breeds in Turkey

Bu çalışma, Zavot (n=48), Doğu Anadolu Kırmızısı (n=40), Simental (n=94) ve İsviçre Esmeri (n=64) sığır ırklarında büyüme hormonu (bGH) geninin AluI RFLP polimorfizminin incelenmesi amacıyla yapılmıştır. Büyüme hormonu-AluI polimorfizmi için toplam 246 baş sığır polimeraz zincir raksiyonu-restriksiyon parça uzunluk polimorfizmi (PZR-RFLP) ile genotiplendirilmiştir. Bu çalışmada, AluI enzim kesimi sonucu iki allel (L ve V) ve üç genotip (LL, VV ve LV) belirlenmiştir. DAK, SIM, İsviçre Esmeri ve Zavot sığır ırkları için allel frekansları; L alleli için sırasıyla 0.775, 0.734, 0.781 ve 0.760; V alleli için sırasıyla 0.225, 0.266, 0.219 ve 0.240 bulunmuştur. Diğer taraftan, genotipik frekanslar LL genotipi için sırasıyla 0.65, 0.57, 0.63 ve 0.63; VV genotipi için 0.10, 0.11, 0.06 ve 0.10; LV genotipi için ise; 0.25, 0.32, 0.31 ve 0.27 bulunmuştur. Çalışılan ırklarda Hardy-Weinberg dengesinden sapma görülmemiştir. Sonuç olarak, bu çalışmada dört sığır ırkında bGH gen polimorfizmi hakkında bilgi verilmiştir. Ayrıca, bu çalışma ile Zavot sığırında bGH gen polimorfizmi varlığı ilk defa bildirilmiştir.

Anahtar Kelimeler:

sığır, Amerikan Esmer Irkı, DNA, Doğu Anadolu Kırmızısı, Simmental, Türkiye, alleller, ırk farklılıkları, sığır ırkları, genler, genetik polimorfizm, genotip, somatotropin

Türkiyedeki dört sığır ırkında büyüme hormonu gen polimorfizmi

This study was conducted to determine DNA-polymorphism of a AluI RFLP at bovine growth hormone (bGH) gene in Zavot (n=48), East Anatolian Red (n=40), Simmental (n=94) and Brown Swiss (n=64) cattle breeds. A total of 246 cattle were genotyped for the bGH-AluI polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the study, two alleles (L and V) and three genotypes (LL, VV and LV) were revealed after than digestion of amplification product with AluI restriction enzyme. Allelic frequencies for EAR, SIM, BS and Zavot breeds were determined as 0.775, 0.734, 0.781 and 0.760 respectively for L allele; 0.225, 0.266, 0.219 and 0.240 respectively for V allele. Otherwise, genotypic frequencies were 0.65, 0.57, 0.63 and 0.63 for LL, 0.10, 0.11, 0.06 and 0.10 for VV, and 0.25, 0.32, 0.31 and 0.27 for LV respectively. A significant deviation from Hardy-Weinberg equilibrium was not observed in the investigated breeds. As a result, this study provided information on the polymorphism of bGH in four cattle breeds. Additionally, this study reported the existence of a genetic polymorphism at bGH gene in Zavot cattle breed for the first time.

Keywords:

cattle, American Brown Swiss, DNA, East Anatolian Red, Simmental, Turkey, alleles, breed differences, cattle breeds, genes, genetic polymorphism, genotypes, somatotropin,

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