EMRAH ŞİMŞEK, ARİF ÇİLOĞLU, SÜLEYMAN AYPAK, Alparslan YILDIRIM, OSMAN SELÇUK ALDEMİR, Ahmet Hakan UNLU, GÖKMEN ZAFER PEKMEZCİ

First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey

Bu çalışmada balıkçılar tarafından Büyük Menderes Nehri'nden yakalanan 30 adet Avrupa yılan balığında (Anguilla anguilla L.) anisakid nematodların varlığı araştırılmıştır. Yılan balıklarında Raphidascaris acus türü nematoda rastlanmıştır. Raphidascaris acus Türkiye sularında yakalanan yılan balıklarında ilk kez bildirilmiştir. İdentifiye edilen parazitlerin internal transcribed spacer gen bölgeleri (ITS-1 ve ITS-2) ve 5.8S rDNA'sı amplifiye edilerek sekans analizleri gerçekleştirilmiştir. Nükleotid sekans karşılaştırmaları sonucu bu nematodların R. acus olduğu doğrulanmıştır. Yılan balığında tespit edilen R. acus izolatının (GenBank erişim numarası: KT633862) ITS gen bölgeleri (ITS-1 ve ITS-2) ve 5.8S bölgesinin ikili hizalamaları sonucunda Hazar Denizi (KM047505) ve Polonya, Vistula Lagünü'nden (AY603537) izole edilmiş R. acus izolatları ile arasında sırasıyla %0.0 ve 1.9 oranlarında tür içi nükleotit farklılığı saptanmıştır. Bu çalışmada Ege Bölgesi akarsularında sularında yakalanan yılan balıklarında (A. anguilla) saptanan R. acus'un ITS gen bölgeleri (ITS-1 ve ITS-2) ve 5.8S rDNA'sı sekanslanarak ilk kez moleküler karakterizasyonu yapılmıştır.

Ege Bölgesi Akarsularında Yakalanan Avrupa Yılan Balıklarında (Anguilla anguilla Linnaeus, 1758) Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae)'un İlk Moleküler Karakterizasyonu

In this study, the presence of anisakid nematodes in the European eels (Anguilla anguilla L.) was investigated. A total of 30 specimens of eels were caught by local fishermen from the Buyuk Menderes River, Turkey. Nematoda species of Raphidascaris acus was found from eels. This is the first record of R. acus from A. anguilla from Turkish waters. The ribosomal DNA (rDNA) internal transcribed spacer regions (ITS-1and ITS-2) and 5.8S of this parasitic species was amplified and sequenced. Also, all the nematodes were identified as R. acus based on nucleotide sequence comparisons. Pairwise comparison between the entire ITS regions and 5.8S of the R. acus isolates of A. anguilla (GenBank accession number: KT633862) and other R. acus isolates from Caspian Sea (KM047505), and Vistula Lagoon, Poland (AY603537) showed differences ranging from 0.0 to 1.9% intraspecific nucleotide differences, respectively. With the present study, R. acus infecting A. anguilla caught off the Aegean Region streams were characterized for the first time by sequencing of the rDNA ITS regions and 5.8S.

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