Immune Cell Counts, Plasma Immunoglobulin Contents and INF-? Gene Expression in Rats Exposed to Bisphenol A

Bu çalışmanın amacı, sıçanlarda immun hücre sayısı, immunoglobulin içeriği ve gama-interferon (INF-?) gen ekspresyonu üzerine bisfenol A'nın (BPA) kademeli düşük doz etkisini değerlendirmekti. Bisfenol A Erkek Wistar ratlara 5 hafta boyunca 15, 30 ve 45 ug/kg canlı ağırlık (c.a.) dozlarında intraperitonal olarak enjekte edildi. Sıçanlar dietil eter ile anestetize sonra kan örnekleri toplandı, plazma ayrıldı ve dalak sıvı azot içine konuldu. Toplam ve diferansiyel beyaz kan hücreleri, antikor titreleri, ve INF-y gen ekspresyonu ölçüldü. Plazma malondialdehit düzeyleri BPA dozları arttıkça doğrusal olarak arttı. Bisfenol A'nın 45 ug/kg c.a. dozu, diğer tedaviler ile karşılaştırıldığında plazma kortizol seviyesinde önemli bir artışla sonuçlandı. BPA dozları arttıkça kırmızı kan hücresi sayımları da doğrusal azaldı. Tedavi grupları arasında eozinofil, monosit ve bazofil sayıları yönünden anlamlı bir fark saptanmadı. BPA dozları arttıkça INF-y gen ekpresyonu da arttı. 45ug/kg c.a. BPA verilen sıçanlar kontrol grubuna göre 3.2 kat daha yüksek INF-y gen ekpresyonuna sahipti. Plazma IgG ve IgA düzeyleri bisfenol dozları arttıkça doğrusal olarak arttı. Plazma IgM düzeyi için tedavi grupları arasında anlamlı bir fark yoktu. Bu çalışmanın sonuçlarına göre; düşük dozlardaki BPA, doz-bağımlı bir şekilde immun tepkilerin artmasına neden olabilir.

Bisfenol A'ya Maruz Bırakılmış Sıçanlarda İmmun Hücre sayısı, Plazma İmmunglobulin İçeriği ve INF-? Gen Ekspresyonu

The purpose of this study was to evaluate the effect of low-dose graded of bisphenol A (BPA) on immune cell counts, immunoglobulin contents and gamma-interferon (INF-?) gene expression in rats. Bisphenol A was injected intraperitoneally to male Wistar rats at doses of 15, 30 and 45 µg/kg body weight for 5 weeks. Rats were anesthetized with diethyl ether and blood samples collected, plasma separated and spleen inserted in liquid nitrogen. Total and differential white blood cells, antibody titers, and gene expression of INF-? were measured. Plasma malondialdehyde levels increased linearly as BPA doses increased. Bisphenol A at dose of 45 µg/kg body weight resulted in significant increase of plasma cortisol level as compared with other treatments. Red blood cell counts decreased linearly as doses of BPA increased. There were no significant differences among treatment for eosinophil, monocytes and basophil counts. The gene expression of INF-? increased as doses of BPA increased. Rats received 45 µg BPA/kg body weight had 3.2 folds higher gene expression of INF-? than the control group. The plasma IgG and IgA levels increased linearly as bisphenol doses increased. There was no significant difference among treatments for plasma IgM level. Based on the results of this study, BPA at low doses may result in increase of immune responses in a dose dependent manner.

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