Isolation of flavobacterium psychrophilum causing rainbow trout fry syndrome and determination of an effective antibacterial treatment in rainbow trout (oncorhynchus mykiss) fry

Gökkuşağı alabalık (Oncorhynchus mykiss) yavrularında Flavobacterium psychrophilum (F. psychrophilum)’un neden olduğu Yavru Gökkuşağı Alabalığı Sendromu (Rainbow Trout Fry Syndrome, RTFS) birçok ülkede yaygın olarak görülen ve ekonomik kayıplara neden olan bakteriyel bir hastalıktır. Bu çalışmada F. psychrophilum suşunun izolasyonu ve identifikasyonu, antibakteriyel duyarlılığı ve etkili antibakteriyel ilaç/ilaçlarla sağaltım seçeneklerinin belirlenmesi amaçlanmıştır. Bu amaçla Ege Bölgesi’nin batısında bulunan özel bir işletmede, doğal yolla F. psychrophilum ile enfekte olduğu tahmin edilen yavruhane bölümünde meydana gelen enfeksiyon sonucunda, canlı ağırlıkları 2-5 g arasında hastalıklı gökkuşağı alabalık yavrusu kullanıldı. İdentifikasyon amacıyla bakterilerin fenotipik, biyokimyasal ve enzim testleri gerçekleştirildi. Kirby-Bauer disk difüzyon yöntemine göre yapılan antibiyogram test sonuçlarına göre etkenin oksitetrasiklin, enrofloksasin, siprofloksasin ve florfenikole duyarlı olduğu belirlendi. Sağaltım gruplarına oksitetrasiklin 75 mg/kg/gün dozda, enrofloksasin, siprofloksasin ve florfenikol 10 mg/kg/gün dozda ve her biri 10 gün süreyle uygulandı. Gökkuşağı alabalık yavrularında klinik bulgular ve ölüm oranları dikkate alındığında; oksitetrasiklin, enrofloksasin ve siprofloksasinin sağaltım için yeterli etkinlik gösteremedikleri, florfenikolün ise diğer sağaltım gruplarına göre enfeksiyonu kontrol almada önemli derecede etkin olduğu ve sağaltım sonunda enfeksiyondan kaynaklı ölüm oranlarını azaltığı belirlendi.

Anahtar Kelimeler:

bakteriyel hastalıklar, siprofloksasin, tespit etmek, ilaç tedavisi, enrofloksasin, florfenikol, izolasyon, oksitetrasiklin, gökkuşağı alabalığı, alabalık

Gökkuşağı alabalıklarında (oncorhynchus mykiss) RTFS’ye (rainbow trout fry syndrome) neden olan flavobacterium psychrophilum etkeninin ızolasyonu ve antibakteriyel sağaltım seçeneğinin belirlenmesi

The bacterial pathogen Flavobacterium psychrophilum (F. psychrophilum) causes rainbow trout (Oncorhynchus mykiss) fry syndorme (RTFS) causes economic losses and widespread in many countries. The aims of the present study are isolation of F. psychrophilum, and determination of antibacterial susceptibility and an effective antibacterial treatment in rainbow trout fry. For this purpose, weighted 2-5 g fry were obtained from a private fish farm in west Aegean region of Turkey. It was estimated according to clinical findings that they were naturally infected with F. psychrophilum originate from the rearing unit. Following fenotipical, biochemical and enzyme tests aimed the identification of bacteria. Kirby-Bauer disc diffusion method was used for determination of antibacterial susceptibility. According to antibiotic susceptibility tests, F. psychrophilum was susceptible to oxytetracycline, enrofloxacin, ciprofloxacin and florfenicol. The antibiotic concentrations in the medicated feeds were to give a dose of 75 mg/kg/day oxytetracycline and 10 mg/ kg/day enrofloxacin, ciprofloxacin and florfenicol each antibiotics for 10 days. When considered the clinical signs and death rates among the infected rainbow trout fry, oxytetracycline, enrofloxacin and ciprofloxacin could not displayed enough efficacy. However, florfenicol showed much higher efficacy for controlling the infection and at the end of the treatment, the death rate caused by the infection was decreased significantly.

Keywords:

bacterial diseases, ciprofloxacin, determination, drug therapy, enrofloxacin, florfenicol, isolation, oxytetracycline, rainbow trout, trout,

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