Establishing the optimum cutting date and additives for pasture Grass silage and ıts Mycotoxin levels

Bu çalışma, çayır otlarından silaj yapılması için en uygun biçim zamanı ve silaj katısı ile silajlardaki mikotoksin rezidülerinin belirlenmesi için yapılmıştır. Çayır otu örnekleri 24 Haziran, 1 ve 8 Temmuz tarihlerinde alınmıştır. Çayır otlarından katkısız, %5 arpa, %5 melas katkılı silajlar yapılmıştır. Silajlar yapıldıktan sonraki 134. günde açılarak kuru madde (KM), ham kül (HK), ham protein (HP), ham selüloz (HS), NDF, ADF, ham yağ (HY), pH, laktik asit (LA), asetik asit (AA), bütirik asit (BA), total aflatoksin, aflatoksin B1 (AFB1), zearalenone yönünden analiz edilmiştir. Silajların HK ve organik madde içerikleri arasında istatistiksel farklılık görülmezken, KM, HP, NDF, ADF, HY, azotsuz öz madde, pH, LA, AA ve BA içeriklerinde farklılık gözlemlenmiştir. Tüm taze çayır otlarında ve silajlarında mikotoksin residüleri bulunmuştur. Silajlardaki total aflatoksin ve AFB1 düzeyi genelde kabul edilebilir sınırların üzerinde iken, zearalenon düzeyi altında bulunmuştur. Bu çalışmada elde edilen bulgular söz konusu tarihlerinin tümünün silaj yapımı için uygun olduğunu, ancak elde edilecek kuru maddenin daha fazla olması bakımından Temmuz ayının ilk iki haftasının daha uygun olduğunu, en iyi katkının küflenmeye karşı gerekli tedbirler alınarak %5 melas olduğunu göstermiştir.

Çayır otu silajları için en uygun biçim zamanı ve silaj katkısının belirlenmesi ve silajlardaki mikotoksin düzeylerinin belirlenmesi

This study was aimed at determining most suitable cutting date and additives for pasture grass for ensiling and their mycotoxin residues. Grass samples were obtained at 24th June; 1st and 8th July. No additives, 5% ground barley or molasses additives silages were made. Silages opened 134 day after ensiling, and analysed for dry matter (DM), crude ash (CA), crude protein (CP), crude fibre (CF), NDF, ADF, ether extract (EE), pH, lactic acid (LA), acetic acid (AA), butyric acid (BA), total aflatoxin, aflatoxin B1 (AFB1), zearalenone. There were no statistical differences in CA and organic matter contents among the silage; however, there were differences in DM, CP, NDF, ADF, EE, nitrogen free extract, pH, LA, AA and BA. Mycotoxins residues were found in all fresh grass and silage samples. Total aflatoxin and AFB1 residues were generally above the acceptable limits in silages, but zearalenone residues were below. These findings suggest that the indicated times in our study are suitable for silage making, but the first two weeks of July is more proper due to the higher dry matter obtained, and best additive is 5% molasses provided that precautions against the fungi are taken in silages with molasses.

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  • 1. McDonald P, Edwards RA, Greenhalgh JFD: Animal Nutrition. 4th Ed. Burnt Mill, Harlow, Essex CM20 2JE, Longman House, England. 1987.
  • 2. Ergun A, Tuncer SD, Colpan I, Yalcin S, Yildiz G, Kucukersan MK, Kucukersan S, Sehu A: Yemler, Yem Hijyeni ve Teknolojisi. Medipress. Ankara, Turkey. 2001.
  • 3. Rinne M, Huhtanen P, Jaakkola S: Digestive processes of dairy cows fed silages harvested at four stages of grass maturity. J Anim Sci, 80, 1986-1998, 2002.
  • 4. Baytok E, Muruz H: The effect of formic acid or formic acid plus molasses additives on the fermentation quality and DM and ADF degradabilities of grass silage. Turk J Vet Anim Sci, 27, 425-431, 2003.
  • 5. Coulombe RA: Biological action of mycotoxins. J Dairy Sci, 76, 880-891, 1993.
  • 6. Scudamore KA, Livesey CT: Occurrence and significance of mycotoxins in forage crops and silage: A review. J Sci Food Agricult, 77, 1-17, 1998.
  • 7. Binder EM, Tan LM, Chin LJ, Handl J, Richard J: Worldwide occurrence of mycotoxins in commodities, feed and feed ingredients. Anim Feed Sci Technol, 137, 265-282, 2007.
  • 8. Kaya I, Oncuer A, Unal Y, Yildiz S: Nutritive value of pastures in Kars district. I. Botanical and nutrient composition at different stages of maturity. Turk J Vet Anim Sci, 28, 275- 280, 2004.
  • 9. AOAC: Official Methods of Analysis. 18th ed. Association of Official Analytical Chemists 2 vol., Arlington, VA, USA. 2004.
  • 10. Van Soest PJ, Robertson JB: Analysis of Forages and Fibrous Foods. A Laboratory Manuel for Animal Science, Cornell University Press. New York, USA, 1985.
  • 11. Leventini MW, Hunt CW, Roffler RE, Casebolt DG: Effect of dietary level of barley-based supplements and ruminal buffer on digestion and growth by beef cattle. J Anim Sci, 68, 4334-4344, 1990.
  • 12. R-Biofarm: Ridascreen® Test Kits. R-Biofarm AG, Darmstat, Germany. 2008.
  • 13. SPSS: SPSS for Windows, Release 10.0, Copyright (SPSS inc. 1989-1999). 27 October 1999.
  • 14. TKB (Tarım ve Köy İşleri Bakanlığı): Yemlerde İstenmeyen Maddeler Hakkında Tebliğ. (Tebliğ No: 2005/3). T.C. Resmi Gazetesi, 05.02.2005 tarih ve 25718 sayısı. 2005.
  • 15. EC: European Commission. Commission Directive No 2002/32/EC of 7 May 2002 on Undesirable Substances in Animal Feed. Official Journal of the European Communities, L 140/10-21, 2002.
  • 16. Oruc HH, Cengiz M, Kalkanli O: Comparison of aflatoxin and fumonisin levels in maize grown in Turkey and imported from the USA. Anim Feed Sci Technol, 128, 337-341, 2006.
  • 17. EC: European Commission. Commission Regulation No 1525/98 of 16 July 1998 Amending Regulation (EC) No 194/97 of 31 January 1997 Setting Maximum Levels for Certain Contaminants in Foodstuffs. Official Journal of the European Communities, L 201/43-46, 1998.
  • 18. Saglam OF: Turk Gıda Kodeksi. Semih Ofset, Ankara. 2003.
  • 19. EC: European Commission. Commission Recommendation No 2006/576/EC of 17 August 2006 on the Presence of Deoxynivalenol, Zearalenone, Ochratoxin A, T-2 and HT-2 and Fumonisins in Products Intended for Animal Feeding. Official Journal of the European Communities, L 229/7-9,2006.
  • 20. Demirel M, Yildirim A: Van yöresinde yetiştirici şartlarında depolanan kaba yemlerde aflatoksin oluşumunun saptanması. YYU Ziraat Fakültesi Tarım Bilimleri Dergisi, 10, 77-83, 2000.
  • 21. Reed KFM, Walsh JR, McFarlane NM, Sprague M: Zearalenone and its presence in pasture. “Animal Production in Australia”. Proceedings of 25th Biennial Conference of the Australian Society of Animal Production, pp. 140-143, 4-8 July 2004, University of Melbourne, Victoria, Australia. 2004.
  • 22. Lindgren S, Lingval P, Kasperson A, Kartzow A, Rydberg E: Effect of inoculants, grain and formic acid on silage fermentation. Swedish J Agric Res, 13, 91-100, 1983.
  • 23. Shaver RD: Practical Application of New Forage Quality Tests. In, Proceedings of the 6th Western Dairy Management Conference. Reno NV, 12-14 March 2003, pp. 17-26. 2003.
  • 24. Petit HV, Flipot PM: Source and feeding level of nitrogen on growth and carcass characteristics of beef steers fed grass as hay or silage. J Anim Sci, 70, 867-875, 1992.
  • 25. Kaya I, Unal Y, Elmali DA: Effects of different additives on the quality of grass silage and rumen degradability and rumen parameters of the grass silage in rams. Kafkas Univ Vet Fak Derg, 15 (1): 19-24, 2009.
  • 26. Kirkland RM, Patterson DC: The effect of quality of grass and maize silage on the intake and performance of beef cattle. Livestock Sci, 100, 179-188, 2006.
  • 27. Gonzalez J, Faria-Marmol J, Rodriguez CA, Martinez A: Effects of ensiling on ruminal degradability and intestinal digestibility of Italian rye-grass. Anim Feed Sci Technol, 136, 38-50, 2007.
  • 28. Driehuis F, Spanjer MC, Scholten JM, Te Giffel MC: Occurrence of mycotoxins in maize, grass and wheat silage for dairy cattle in the Netherlands. Food Additives and Contaminants, Part B, 1, 41-50, 2008.
  • 29. Marley CL, Fychan R, Fraser MD, Sanderson R, Jones R: Effects of feeding different ensiled forages on the productivity and nutrient-use efficiency of finishing lambs. Grass Forage Sci, 62, 1-12, 2007.
  • 30. Zimmerman C: Silage Fermentation Analyses. http:// www.blueseal.,com/techtalks/28/. Accessed: 15th April 2008.
  • 31. O’Brien M, O’Kiely P, Forristal PD, Fuller HT: Visible fungal growth on baled grass silage during the winter feeding season in Ireland and silage characteristic associated with the occurrence of fungi. Anim Feed Sci Technol, 139, 234-256,2007.
  • 32. Dewhurst RJ, Fisher WJ, Tweed JKS, Wilkins RJ: Comparison of grass and legume silages form milk production. 1. Production responses with different levels of concentrate. J Dairy Sci, 86, 2598-2611, 2003.
  • 33. Speijers MHM, Fraser MD, Theobald VJ, Haresign W: Effect of ensiled forage legumes on performance of store finishing lambs. Anim Feed Sci Technol, 120, 203-216, 2005.
  • 34. Cai Y, Benno Y, Ogawa M, Kumai S: Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage. J Dairy Sci, 82, 520-526, 1999.
  • 35. Sahin K, Cerci HI, Guler T, Sahin N, Kalender H, Celik S: Farklı silaj katkı maddelerinin yaş şeker pancari posasi silaji kalitesine etkileri. Turk J Vet Anim Sci, 23, 285-292, 1999.
  • 36. Lepom P, Baath H, Knabe O: Occurrence of Fusarium species and their mycotoxins in maize 3. The influence of silaging on the zearalenone content of CCM maize. Arch Anim Nutr, 38, 817-823, 1998.
  • 37. Yildiz G: Türkiye’de üretilen bazı kaba yemlerde aflatoksin düzeyinin belirlenmesi. III. Hayvan Besleme Kongresi. 07-10 Eylül 2005, pp. 553-556. Adana, Türkiye. 2005.
Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
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