Hakan KOCAMIŞ, EBRU KARADAĞ SARI, Mümtaz NAZLI, Nurhayat GÜLMEZ, ŞAHİN ASLAN, TURGAY DEPREM

Immunohistochemical distribution of ınsulin-, glucagon- and somatostatin-containing cells in the pancreas of the rat (Wistar albino)

Bu çalışmanın amacı insulin-, glukagon-, ve somatostatin-içeren hücrelerin rat pankreasında bölgesel olarak dağılımının belirlenmesidir. Doku örnekleri, derin etil anestezisi altındaki 10 adet erişkin rattan alındı. Doku kesitleri genel görünüm için Crossmon’ın bağ doku boyasıyla boyandı. Kesitlere insulin-, glukagon-, ve somatostatin içeren hücrelerin dağılımının belirlenmesi için standart Avidin-biotin complex (ABC) metodu uygulandı. İnsulin içeren B hücrelerinin adacıkların merkezinde yerleştiği görüldü. Glukagon-içeren A hücreleri ve somatostatin-içeren D hücreleri adacıkların periferinde yerleşmişti. Somatostatin-içeren hücreler Langerhans adacıklarında az sayıdaydı. Sonuç olarak elde edilen bulgular sıklıkla diabet ya da adacık hücre tümörleri gibi hastalıklarda hayvan modeli olarak kullanılan ratların pankreasında yapılan çalışmalara katkıda bulunacaktır.

Anahtar Kelimeler:

insülin, pankreas, glukagon, dağılım, sıçan, immünohistokimya, doku kimyası, somatostatin

Rat (Wistar albino) pankreasında insulin-, glukagon- ve somatostatin-içeren hücrelerin immunohistokimyasal olarak dağılımı

The aim of the present study was to determine the regional distribution of the insulin-, glucagon-, and somatostatincontaining cells in the pancreas of rat. Tissue samples were obtained under deep ethyl ether anaesthesia from 10 adult rat (Wistar albino). Tissue sections were stained with Crossmon’s connective tissue staining for general observations. Sections were further processed for standart immunohistochemical techniques using the avidin- biotin-complex method for the distribution of insulin-, glucagon-, and somatostatin-containing cells. It was monitored that insulin-containing B cells were located central regions of the islets of Langerhans whereas glucagon- containing A cells and somatostatin- containing D cells were located peripheral regions of the islets. Somatostatin- containing cells were rare in the islets of Langerhans. Our findings make additional contribution to those of pancreatic studies of rats which have been often used as an animal model in the studies dealing with diabetes or islet cell tumours.

Keywords:

insulin, pancreas, glucagon, distribution, rats, immunohistochemistry, histochemistry, somatostatin,

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