Erzurum yöresinde satışa sunulan kırmızı etlerde 17 β-östradiol, dietilstilbestrol ve zeranol kalıntılarının araştırılması

Bu çalışmada sığır etlerinde anabolik amaçlı kullanılan 17 β-östradiol, dietilstilbestrol ve zeranol hormon kalıntılarının belirlenmesi amaçlandı. Bu çalışmada test materyali olarak kullanılan toplam 204 örnek (51 boyun, göğüs, but ve idrar örnekleri) Erzurum’daki mezbahalardan temin edildi. Ekstrakte edilen örneklerdeki hormon kalıntıları Enzyme-Linked Immunosorbent Assay (ELISA) ile belirlendi. Çalışmada, 16 et örneğinde 17 β-östradiol (4), dietilstilbestrol (4) ve zeranol (8) ≥0.5 ng/kg, diğer taraftan 6 idrar örneğinde dietilstilbestrol (4) ve zeranol (2) ≥0.5 ng/L düzeyinde bulundu. Bununla birlikte, örneklerdeki hormonların belirlenmesi ve konsantrasyonları bağımsız akredite labotaruvarda (TUBİTAK-MAM) LC-MS-MS ile doğrulandı. Sonuç olarak et ve et ürünlerinde hormon bulunması tüketiciler için zararlı olduğundan dolayı, Türkiye’de anabolik maddelerin kullanımı sıklıkla kontrol edilmelidir.

Anahtar Kelimeler:

dietilstibestrol, Türkiye, zeranol, kalıntı, et, östradiol

Investigation of residual 17 β-estradiol, diethylstilbestrol, and zeranol in red meat sold in Erzurum province, Turkey

The aim of this study was to detect hormone residues such as 17 β-estradiol, diethylstilbestrol, and zeranol used anabolic agents in bovine meat. In the study, a total of 204 samples used as test materials (51 neck, thoracic, leg meat and urine samples) were obtained from local abattoirs in Erzurum. The hormone residues in the sample extracts were detected by Enzyme-Linked Immunosorbent Assay (ELISA). 17 β-estradiol (4), diethylstilbestrol (4), and zeranol (8) were detected in 16 meat samples at ≥0.5 ng/kg, on the other hand, diethylstilbestrol (4) and zeranol (2) were detected in 6 urine samples at ≥0.5 ng/L in this study. However, concentrations and identities of the hormones in the samples were confirmed by independent accredited laboratory (TUBITAK-MAM) via LC–MS–MS. In conclusion, since presence of hormones in meat and meat products is harmful for consumers, utilization of anabolic agents must be strictly controlled in Turkey.

Keywords:

diethylstilbestrol, Turkey, zeranol, residues, meat, estradiol,

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