SELİM ALÇAY, BURCU ÜSTÜNER, İBRAHİM ÇAKMAK, Selvinar ÇAKMAK, ZEKARİYA NUR

Effects of various cryoprotective agents on post-thaw drone semen quality

Bu çalışmanın amacı, farklı kriyoprotektanların eritme sonrası arı spermasının motilite ve plazma membran bütünlüğü üzerine etkisini değerlendirmektir. Sperma dört koloniden bulunan olgun arılardan (16 gün ve üzeri) alındı. Toplanan sperma final konsantrasyonu 1/5 olacak şekilde %0 kriyoprotektan içermeyen (kontrol), %6 gliserol, %6 etilen glikol ve %6 1,2 Propanediol ile iki aşamalı sulandırıldı. Ekilibrasyon sonrası sperma 0.25 mllik payetlerde donduruldu. Sulandırma sonrası, ekilibrasyon ve eritme sonrası aşamalarda motilite (%) ve HOS testi yapıldı. Eritme sonrası motilite ve plazma membran bütünlüğüne bakıldığında kullanım öncülüğüne göre kriyoprotektanları DMSO, Etilen Glikol, Gliserol ve 1,2 Propanediol olarak sıralayabiliriz. Sonuç olarak, eritme sonrası motilite ve plazma membran bütünlüğü bakımından; DMSO ile dondurulan sperma, kontrol, gliserol, etilen glikol, 1,2 propanediol gruplarıyla karşılaştırıldığında en iyi sonucu vermiştir (P

Farklı kriyoprotektanların eritme sonrası arı sperm kalitesi üzerine etkileri

The aim of the present study was to evaluate the efect of diferent cryoprotectants on post-thaw semen motility and plasma membrane integrity of drone spermatozoa. Semen was obtained from mature drones (16 days or older) harvested from four colonies. Collected semen was diluted to a fnal concentration of 1/5 (semen/extender) in 0% cryoprotectant (control), 6% glycerol, 6% Ethylene Glycol, 6% 1,2 propanediol or 6% DMSO using a two-step dilution method. The equilibrated semen was frozen in 0.25-ml straws. The percentage of sperm motility and swollen tails (HOST) spermatozoa were evaluated following dilution with extender A (non-cryoprotectant), equilibration and post-thaw stages. In terms of post-thaw motility and plasma membrane integrity recovery we can rank the used cryoprotectant as DMSO, Ethylene Glycol, Glycerol and 1,2 Propanediol; respectively. In conclusion, post-thaw sperm motility and plasma membrane integrity of the present study was signifcantly better when sperm was frozen in DMSO with respect to control, glycerol, ethylene glycol, 1,2 propanediol (P<0.05).

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