Harun ALP, İsmail AYTEKİN, Hasan ESEN, Kemal BAŞARALI, Seval KUL

Effects of caffeic acid phenethyl ester, ellagic acid, sulforaphane and curcumin on diazinon induced damage to the lungs, liver and kidneys in an acute toxicity rat model

Bu çalışmanın amacı kafeik asit fenetil ester (CAPE), elajik asit (EA), Sulforafan (SFN) ve Kurkumin (CUR)’in, diazinon (DI)’un toksik etkilerine karşı muhtemel koruyucu etkilerini araştırmaktır. Çalışmada 60 adet Sprague Dawley sıçan eşit şekilde ve rastgele 10 gruba ayrıldı. Kontrol grupları; kontrol, CAPE, EA, SFN ve CUR grupları olarak 5 gruba ayrıldı. Geri kalan 5 grup ise çalışma grupları olarak; DI, DI + CAPE, DI + EA, DI + SFN ve DI + CUR gruplarına ayrıldı. Hayvanlar ilaç uygulamalarından 24 saat sonra sakrifiye edildi. DI, asetil kolin esteraz (AChE) aktivitelerine azaltıcı (P

Akut toksisite sıçan modelinde diazinonun neden olduğu akciğer, karaciğer ve böbrek hasarı üzerine kafeik asit fenetil ester, elajik asit, Sulforafan ve Kurkumin’in etkileri

The aim of this study was to investigate the possible protective effects of caffeic acid phenethyl ester (CAPE), ellagic acid (EA), sulforaphane (SFN) and curcumin (CUR) against the toxic effects of diazinon (DI). Sixty Sprague Dawley rats were randomly divided into 10 groups. Five groups were allocated as control groups comprising unmedicated control, CAPE, EA, SFN and CUR control groups. The remaining five groups were the study groups comprising DI, DI + CAPE, DI + EA, DI + SFN, and DI + CUR groups. The animals were sacrified 24 h after drug administrations. DI caused a decrease in acetyl cholinesterase (AChE) activity (P<0.05) and increases in γ-glutamyltransferase (GGT) and amylase activities. It also damaged the kidney, liver, and lung tissues. The negative effects of DI on these enzymes were confirmed histopathologically. Also, CAPE, EA, SFN and CUR reduced amylase and GGT activities and caused an increase in the AChE activities that were increased due to the toxic effects of DI. Thus, it was determined bio- chemically and histopathologically that these medication reduced the degenerative toxic effects created by DI in the lung, liver and kidney tissues. These findings led us to believe that CAPE, EA, SFN and CUR may be used as protective medicines in acute DI intoxication.

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