NADİM YILMAZER, ESİN GÜVEN, Sadiye Kaplan KUCUK, Katja DITTMAR, Berit BANGOURA, Sirri KAR

Could vital dyes be used to determine the degree of the time dependent viability changes in Cryptosporidium parvum oocysts?

Bu çalışmada, antimikrobiyal destekli PBS içerisinde +4oC’de muhafaza edilen pürifiye Cryptosporidium parvum oocystlerindeki canlılık değişimi vital boyalarla (DAPI/PI) belirlenmeye çalışılmıştır. Denemeler, vital boyaların oocyst canlılığındaki değişimi ortaya koyabildiğini ve ayrıca iyi değerlendirildiği taktirde, canlılıktaki söz konusu değişimin derecesinin de, hücre kültürü-PCR uygulamalarına benzer şekilde belirlenebileceğini göstermiştir.

Anahtar Kelimeler:

ookist, insan hastalıkları, polimeraz zincir reaksiyonu, protozoa enfeksiyonları, yaşayabilme, Cryptosporidium parvum, antiprotozoal ajanlar, hücre kültürü, cryptosporidiosis, boyalar

Cryptosporidium parvum oocystlerindeki zamana bağlı canlılık değişim derecesi vital boyalarla belirlenebilir mi?

The present study was undertaken to determine time dependent viability changes of purified Cryptosporidium parvum oocysts, stored in antimicrobial-supplemented PBS at +4oC, using vital dyes (DAPI/PI). The trials demonstrated that vital dyes could provide estimation of oocyst viability, and furthermore, if interpreted correctly, they could be used to determine the degree of the viability in Cryptosporidium parvum oocysts as cell culture-PCR assay is used.

Keywords:

oocysts, human diseases, polymerase chain reaction, protozoal infections, survival, Cryptosporidium parvum, antiprotozoal agents, cell culture, cryptosporidiosis, dyes,

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