Abdul MALIK, Muhammad SYARIFDJAYA, Aam GUNAWAN, Siti ERLINA, Achmad JAELANI, Dadang Budi WIBOWO

Effect of Fish Oil Addition to the Skim Milk-egg Yolk Extender on the Quality of Frozen-thawed Bali Bull Spermatozoa

Bu çalışma sperma sulandırıcısına katılan balık yağının Bali boğa spermatozoonlarının çözdürme sonrası kalitesi üzerine etkisini araştırmak amacıyla yapıldı. Süt tozu-yumurta sarısı sulandırıcısına 0 (kontrol), 50, 100, 150 ve 200 mg/100 mL dozunda balık yağı ilave edildi. 100 mg/100 mL balık yağı ilavesi kontrol grubu ile karşılaştırıldığında hem canlı sperm oranını hem de motiliteyi önemli derecede (P

Süt Tozu-Yumurta Sarısı Sulandırıcısına Balık Yağı İlavesinin Dondurulmuş- Çözdürülmüş Bali Boğa Spermatozoonlarının Kalitesi Üzerine Etkisi

This study was conducted to investigate the effect of fish oil addition to the semen extender on post-thaw quality of Bali bull spermatozoa. Fish oil was added at the doses of 0 (control), 50, 100, 150 and 200 mg/100 mL to the skim milk-egg yolk extender. Supplementation of fish oil at the dose of 100 mg/mL significantly (P

PDF

___

1. Demir K, Öztürk Bakırer G, Cirit Ü, Bozkurt HH, Aktaş A, Birler S, Ak K, Pabuçcuoğlu S: Effects of cooling rate on membrane integrity and motility parameters of cryopreserved ram spermatozoa. Kafkas Univ Vet Fak Derg, 21 (1): 61-67, 2015. DOI: 10.9775/kvfd.2014.11726
2. Isachenko E: Vitrification of mammalian spermatozoa in the absence of cryoprotectans: from past practical difficulties to present. Reprod Biol, 6, 191-200, 2003. DOI: 10.1016/S1472-6483(10)61710-5
3. Khalili B, Farshad A, Zamiri MJ, Rashidi A, Fazeli P: Effects of sucrose and trehalose on the freezability of Markhoz goat spermatozoa. Asian Australas J Anim Sci, 22, 1614-1619, 2009. DOI: 10.5713/ajas.2009.90286
4. Purdy PH: A review on goat sperm cryopreservation. Small Ruminant Res, 63, 215-225, 2006. DOI: 10.1016/j.smallrumres.2005.02.015
5. Hammerstedt RH: Maintenance of bioenergetic balance in sperm and prevention of lipid peroxidation: A review of the effect on design of storage preservation systems. Reprod Fertil Dev, 5, 675-690,1993. DOI: 10.1071/RD9930675
6. Mandal R, Damodar B, Jitamanyu C: Role of membrane lipid fatty acids in sperm cryopreservation. Adv Androl, 2014 (2014), Article ID: 190542. DOI: 10.1155/2014/190542
7. Stoeckel K, Nielsen LH, Fuhrmann H, Bachmann L: Fatty acid patterns of dog erythrocyte membranes after feeding of a fish-oil based DHA-rich supplement with a base diet low in n-3 fatty acids versus a diet containing added n-3 fatty acids. Acta Vet Scand, 6, 53-57, 2011. DOI: 10.1186/1751-0147-53-57
8. Nichols PD, Glencross B, Petrie JR, Singh SP: Readily available sources of long-chain omega-3 oils: Is farmed Australian seafood a better source of the good oil than wild-caught seafood. Nutrients, 6, 1063-1079, 2014. DOI: 10.3390/nu6031063
9. Rooke JA, Shao CC, Speake BK: Effects of feeding tuna oil on the lipid composition of pig spermatozoa and in vitro characteristics of semen. Reproduction, 121 (2): 315-322, 2001.
10. Blesbois E, Douard V, Germain M, Boniface P, Pellet F: Effects of n-3 polyunsaturated dietary supplementation on the reproductive capacity of male turkeys. Theriogenology, 61, 537-549, 2004. DOI: 10.1016/S0093- 691X(03)00207-3
11. Samadian F, Towhidi A, Rezayazdi K, Bahreini M: Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm. Animal, 4 (12): 2017-2022, 2010. DOI: 10.1017/S1751731110001308
12. Castellano CA, Audet I, Laforest JP, Matte JJ, Suh M: Fish oil diets alter the phospholipid balance, fatty acid composition, and steroid hormone concentrations in testes of adultpigs.Theriogenology, 76, 1134- 1145, 2011. DOI: 10.1016/j.theriogenology.2011.05.022
13. Aksoy Y, Aksoy H, Altinkaynak K, Aydin HR, Ozkan A: Sperm fatty acid composition in subfertile men. Prostaglandins Leukot Essent Fatty Acids, 75, 75-79, 2006. DOI: 10.1016/j.plefa.2006.06.002
14. Am-in N, Kirkwood RN, Techakumphu M, Tantasuparuk W: Lipid profiles of sperm and seminal plasma from boars having normal or low sperm motility. Therigenology, 75, 897-903, 2011. DOI: 10.1016/j. theriogenology.2010.10.032
15. Kaeoket K, Sang-urai P, Thamniyom A, Chanapiwat P, Techakumphu M: Effect of docosahexaenoic acid on quality of cryopreserved boar semen in different breeds. Reprod Domest Anim, 45, 458-463, 2010. DOI: 10.1111/j.1439-0531.2008.01239.x
16. Maldjian A, Pizzi F, Gliozzi T, Cerolini S, Penny P, Noble R: Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology, 63, 411-421, 2005. DOI: 10.1016/j.theriogenology. 2004.09.021
17. Chanapiwat P, Kaeoket K, Tummaruk P: Effects of DHA-enriched hen egg yolk and l-cysteine supplementation on quality of cryopreserved boar semen. Asian J Androl, 11, 600-608, 2009. DOI: 10.1038/aja.2009.40
18. Evans G, Maxwell WMS: Salamon’s Artificial Insemination of Sheep and Goats. University Press, Sydney, NSW, Australia, 1987.
19. Mammoto A, Masumoto N, Tahara M, Ikebuchi Y, Ohmichi M, Tasaka K, Miyake A: Reactive oxygen species block sperm-egg fusion via oxidation of sperm sulfhydryl proteins in mice. Biol Reprod, 55, 1063- 1068, 1996.
20. Aitken RJ: Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev, 7, 659-668, 1995. DOI: 10.1071/RD9950659
21. Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P, Jennings Z, Irvine DS: Relative impact of oxidative stress on the functional competence and genomic integrity of humanspermatozoa. Biol Reprod, 59, 1037- 1046,1 998. DOI: 10.1095/biolreprod59.5.1037
22. Esteso MC, Fernández SMR, Soler AJ, Garde JJ: Head dimensions of cryopreserved red deer spermatozoa are affected by the thawing procedure. Cryoletters, 24, 261-268, 2003.
23. Munoz OVL, Amirat-Briand T, Diaz L, Va´squez E, Schmidt S, Desherces M, Anton D, Bencharif D, Tainturier D: Effect of semen dilution to low-sperm number per dose on motility and functionality of cryopreserved bovine spermatozoa using low-density lipoproteins (LDL) extender: Comparison to Triladyl® and Bioxcell®. Theriogenology, 71, 895- 900, 2009. DOI: 10.1016/j.theriogenology.2008.10.010
24. Abdi-Benemar H, M Jafaroghli B, Khalili, MJ Zamiri, H. Ezazi, AA Shadparvar: Effects of DHA supplementation of the extender containing egg yolk and - tocopherol on the freezability and post-thawing fertility of ram semen. Small Ruminant Res, 130, 166-170, 2015. DOI: 10.1016/j. smallrumres.2015.06.013
25. Wathes DC, Abayasekara DR, Aitken RJ: Polyunsaturated fatty acids in male and female reproduction. Biol Reprod, 77, 190-201, 2007. DOI: 10.1095/biolreprod.107.060558
26. Conquer JA, Martin JB, Tummon I, Watson L, Tekpetey F: Effect of DHA supplementation on DHA status and sperm motility in asthenozoospermic males. Lipids, 35, 149-154, 2000. DOI: 10.1007/BF02664764
27. Kandelousi SMA, Arshami J, Naserian AA, Abavisani A: The effects of addition of omega-3, 6, 9 fatty acids on the quality of bovine chilled and frozen-thawed sperm. Open Vet, 3 (1): 47-52, 2013.
28. Chenoweth PJ: Genetic sperm defects. Theriogenology, 64, 457-468, 2005. DOI: 10.1016/j.theriogenology.2005.05.005