Liuming ZHANG, Tariq SOHAIL, Yongjun LI, Yanhu WANG, Changjiang CHU, Yunkui FENG, Jinliang MA

Eff ect of Diff erent Extenders on the Sperm Quality Parameters of Hu Ram Semen Preserved at 16ºC

The purpose of this experiment was to determine the eff ects of diff erent extenders on the sperm quality parameters of Hu ram semen preserved at 16ºC. The quality parameters studied include total sperm motility, progressive motility, survival time, plasma membrane integrity, acrosome integrity and some kinematic parameters, such as the curvilinear velocity. Each ram ejaculated approximately twenty times and ejaculates were collected every two days interval during breeding season. Three Hu rams were used in experiments. The ejaculates were pooled and diluted (1:10) with extenders A (Tris-Fructose based), B (Fructose-Sodium Citrate based), C (Glucose based), D (Fructose based) and E (Control, Physiological saline solution) and then stored at 16ºC. The above parameters were detected every 24 h. The total sperm motility, progressive motility, acrosome integrity and some kinematic parameters of extender A were the highest compared to those of other extenders and decreased slowly within 24 to 144 h. The eff ective survival time of sperm preserved in extender A was 74.50±4.82 h, and the total survival time was 412.67±2.52 h, which was significantly higher than those of the other four extenders (P≤0.05). The acrosome integrity of extender A was the highest within 24 to 144 h and significantly higher than those of the other extenders within 48 to 144 h (P≤0.05). Compared with the other extenders, extender A had numerically the highest plasma membrane integrity within 24 to 96 h of preservation (P>0.05). In conclusion, extender A improved the sperm quality of Hu ram semen, which could be used for artificial insemination for up to 144 h of preservation.

Farklı Sperm Sulandırıcılarının 16°C’de Saklanan Hu Koç Sperm Kalitesi Parametreleri Üzerine Etkisi

Bu çalışmanın amacı, farklı sperm sulandırıcılarının 16ºC’de saklanan Hu koç sperm kalitesi parametreleri üzerindeki etkilerini belirlemekti. İncelenen kalite parametreleri arasında toplam sperm motilitesi, progresif motilite, canlı kalma süresi, plazma membran bütünlüğü, akrozom bütünlüğü ve eğrisel hız gibi bazı kinematik parametreler bulunuyordu. Her koçtan yaklaşık yirmi kez sperm alındı ve üreme mevsimi boyunca koçlardan iki günde bir ejekülat toplandı. Deneylerde üç Hu koçu kullanıldı. Ejekülatlar havuz oluşturulduktan sonra, 1:10 oranında sırasıyla A (Tris-Fruktoz bazlı), B (Fruktoz-Sodyum Sitrat bazlı), C (Glukoz bazlı), D (Fruktoz bazlı) ve E (Kontrol, Fizyolojik tuzlu su) sulandırıcıları ile seyreltildi ve sonra 16ºC’de muhafaza edildi. Adı geçen parametreler her 24 saatte bir tespit edildi. A sulandırıcısının kullanıldığı gruptaki toplam sperm motilitesi, progresif motilite, akrozom bütünlüğü ve bazı kinematik parametreleri diğer sulandırıcılara oranla en yüksek saptandı ve bu özellikler 24 ile 144 saat içinde yavaş yavaş azaldı. A sulandırıcısının kullanıldığı gruptaki spermlerin etkin canlılık süresi 74.50±4.82 saat ve toplam canlılık süresi 412.67±2.52 saat iken, bu değerler diğer dört sulandırıcıdan çok daha yüksek saptandı (P≤0.05). A sulandırıcısının kullanıldığı gruptaki spermlerin akrozom bütünlüğü 24 ile 144 saat içinde en yüksekti ve bu değerler diğer sulandırıcıların 48 ile 144 saat içinde sahip oldukları değerlerden çok daha yüksekti (P≤0.05). Diğer sulandırıcılarla karşılaştırıldığında A sulandırıcısının, 24 ile 96 saatlik sperm muhafazasında sayısal olarak en yüksek plazma membran bütünlüğüne sahip olduğu saptandı (P >0.05). Sonuç olarak, A sperm sulandırıcısının, suni tohumlama için kullanılmak üzere 144 saate kadar muhafaza süresince sperm kalitesini artırdığı belirlendi.

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