Mehrzad AFLATOUNI, Negar PANAHI, Pejman MORTAZAVI, Bahar SHEMSHADI, Shapour KAKOOLAKI

Hepatoprotective Activity of Silymarin in Combination with Clorsulon Against Fasciola hepatica in Naturally Infected Sheep

Fasciolosis is a parasitic infection with socioeconomic implications that causes essential health problems in animals throughout the world. The current study intended to evaluate the hepatoprotective activity of silymarin alone and in combination with clorsulon in sheep naturally infected with Fasciola hepatica. For this purpose, a total of 40 sheep (Sangsari breed) were divided into five groups as follows: 1) the healthy sheep without treatment (the negative control), 2) the infected sheep without treatment (the positive control), 3) the infected sheep treated with silymarin (280 mg orally from the day 1 to 14), 4) the infected sheep treated with clorsulon (7.5 mg/kg orally on the days 5, 10, and 15), and 5) the infected sheep treated with silymarin + clorsulon. The assessed serum parameters included total bilirubin, total protein, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT). Additionally, histopathological changes in naturally F. hepatica-infected sheep and controls were investigated using light microscopy. Biochemical analysis showed significantly lower levels of AST, ALT, ALP, GGT, bilirubin, protein, and albumin in infected animals than in the healthy group (P

Fasciola hepatica İle Doğal Enfekte Koyunlarda Silymarinin Klorsulon İle Kombinasyonunun Hepatoprotektif Aktivitesi

Fasciolosis, tüm dünyada hayvanlarda temel sağlık sorunlarına neden olan ve sosyoekonomik etkilere sahip paraziter bir enfeksiyondur. Bu çalışma, Fasciola hepatica ile doğal olarak enfekte olmuş koyunlarda silymarinin tek başına ve klorsulon ile kombinasyon halinde kullanımının hepatoprotektif aktivitesini değerlendirmeyi amaçlamıştır. Bu amaçla toplam 40 koyun (Sangsari ırkı) beş gruba ayrıldı: 1) tedavi görmeyen sağlıklı koyunlar (negatif kontrol), 2) tedavi görmemiş enfekte koyunlar (pozitif kontrol), 3) silymarin ile tedavi edilen enfekte koyunlar (1. günden 14. güne kadar oral yoldan 280 mg), 4) klorsulon ile tedavi edilen enfekte koyunlar (5, 10 ve 15 günlerde ağızdan 7.5 mg/kg) ve 5) silymarin + klorsulon ile tedavi edilen enfekte koyunlar. Serum parametreleri olarak total bilirubin, toplam protein, albümin, alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), alkalin fosfataz (ALP) ve gama-glutamil transferaz (GGT) incelendi. Bunun yanı sıra, doğal olarak F. hepatica ile enfekte ve kontrol grubundaki koyunlarda histopatolojik değişiklikler ışık mikroskobu kullanılarak incelendi. Analiz sonuçları, enfekte hayvanlarda AST, ALT, ALP, GGT, bilirubin, protein ve albümin düzeylerinin sağlıklı gruba göre anlamlı derecede düşük olduğunu gösterdi (P

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