Wentao MA, Yi Zheng WANG, Fei GAO, Mengxia NING, Ahua LIU, Yanyan LI, Yang GAO, Ping LU, Dekun CHEN

Development of a Monoclonal Antibody Against Bovine α-casein to Evaluate Functional Status of Mammary Epithelial cells During Mastitis

Mastitis is a widespread disease in dairy animals and causes huge economic losses around the globe. Mastitis affects the health of animals and also reduces the protein content in the milk. In lactating animals, the surface of mammary gland is coated and lined with mammary epithelial cells (MECs). The functional status of MECs can be evaluated by determining the expression of caseins. The total content of caseins can largely reflect the quality of milk. In the previous literature, there is no general information about the milk quality and expressions of casein in mastitis. The current study was designed to determine if there is any correlations between mastitis and α-casein expression in MECs. We prepared a hybridoma cell line that produces antibody against bovine α-casein to evaluate the casein expression status in vitro on the MECs during lipopolysaccharide (LPS)-induced mastitis. The results showed that the expression of α-casein has not changed significantly under the stimulation of LPS. Our study established a useful tool to determine the expression profile of α-casein in bovine MECs. Furthermore, the result indicated that the expression level of α-casein remained stable during mastitis.

Mastitis Süresince Meme Epitel Hücrelerinin Fonksiyonel Durumunu Değerlendirmek Amacıyla Sığır α-kazein Proteinine karşı Monoklonal Antikor Geliştirilmesi

Mastitis sütçü hayvanlarda yaygın bir hastalıktır ve dünya çapında büyük ekonomik kayıplara neden olmaktadır. Mastitis hayvanların sağlığını etkilemekte ve sütteki protein miktarını düşürmektedir. Laktasyondaki hayvanlarda, meme bezlerinin yüzeyleri meme epitel hücreleri ile döşelidir. Meme epitelyum hücrelerinin fonksiyonel durumu kazein ekspresyonu belirlenerek değerlendirilebilir. Total kazein miktarı büyük ölçüde sütün kalitesini yansıtmaktadır. Literatürde süt kalitesi ve mastitiste kazein ekspresyonu hakkında bir bilgi bulunmamaktadır. Bu çalışma, mastitis ile meme epitelyum hücrelerinde α-kazein ekspresyonu arasında bir ilişki bulunup bulunmadığını belirlemek amacıyla yapılmıştır. Lipopolisakkarit (LPS) ile oluşturulmuş mastitis süresince meme epitelyum hücrelerinde in vitro kazein ekspresyonunu değerlendirebilmek amacıyla sığır α-kazeinine karşı antikor üreten hibridoma hücre hattı hazırlanmıştır. Elde edilen sonuçlar, α-kazein ekspresyonunun LPS stimulasyonu altında anlamlı derecede değişmediğini göstermiştir. Bu çalışma sığır meme epitel hücrelerinde α-kazein ekspresyonunu belirlemek amacıyla yararlı olmuştur. Aynı zamanda, sonuçlar mastitis süresince α-kazein ekspresyon seviyesinin stabil olduğunu göstermiştir.

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