Leyla GÜLER, Kadri GÜNDÜZ, S. Asuman SARIŞAHİN

Capsular typing and antimicrobial susceptibility of pasteurella multocida isolated from different hosts

Bu çalışmada 2001-2012 yılları arasında sığır, koyun, keçi ve tavşan gibi değişik hayvan türlerinden izole edilen Pasteurella multocida suşlarının multipleks PCR ile identifikasyonu ve kapsüler serogrupları (A, B, D, E ve F) araştırıldı. Toplam 75 suşun 36sı (19 akciğer, 12 burun svabı, 5 süt) sığır, 33ü koyun, 3ü keçi ve 3ü de tavşan akciğerlerinden izole edildi. İki işletmeden alınan 9 (6 ve 3er adet) sığır burun svabı dışındaki örneklerin her biri farklı işletmelerden gelen hayvanlara aitti. Konvansiyonel yöntemlerle P. multocida olarak identifiye edilen suşların tümü multipleks PCR ile doğrulandı. Kapsüler tiplendirme sonucu 36 sığır suşunun 33 (%91.6)ü serogroup A; 33 koyun suşunun 11 (%33.3)i serogrup D, 2 (%6.0)si A, 1(%3.0)i F olarak belirlendi ve 19 (%57.5)u tiplendirilemedi; 3 keçi suşunun 2si serogrup D olarak tiplendirildi; 3 tavşan suşunun hiçbiri tiplendirilemedi. Toplam 26 (%34.6) suş tiplendirilemedi. Serogrup B ve Eye ait suş tespit edilmedi. Disk difüzyon yöntemiyle 50 suşa yapılan antimikrobiyal duyarlılık test sonucunda suşların tümü ceftiofur, enrofloxacin, florfenicol ve trimethoprim-sulfamethoxazolee duyarlı bulundu. Spectinomycin ve tetracyclinee 6 (%12.0)şar suş, tilmicosin ve erythromycine 3 (%6.0)er, suş dirençli bulundu. Dirençli 11 (10 sığır, 1 koyun) suşun 7sinde çoklu direnç (4 suşta tetracycline + spectinomycin, 2 suşta tilmicosin + erythromycin, 1 suşta tetracycline + tilmicosin + erythromycin) tespit edildi.

Farklı hayvan türlerinden izole edilen pasteurella multocida suşlarının kapsüler tiplendirilmesi ve antimikrobiyal duyarlılıkları

In this study, 75 Pasteurella multocida isolates from different animal species of cattle, sheep, goat and rabbit isolated during 2001-2012 were identified and characterized by multiplex PCR for 5 capsular types of A, B, D, E, and F. Thirty six of isolates were isolated from cattle (19 lungs, 12 nasal swabs and 5 milk samples), 33 from sheep lungs, 3 from goat lungs and 3 from rabbit lungs. Each isolates was obtained from different herds/flocks except 9 bovine nasal swab isolates (6 and 3), which were from two herds. All P. multocida isolates identified by conventional methods were confirmed by multiplex PCR. Thirty three (91.6%) of 36 bovine isolates were serogroup A; 11 (33.3%) of 33 sheep isolates were serogroup D, 2 (6.0%) were serogroup A, 1 (3.0%) was serogroup F and 19 (57.5%) were untypable; 2 of 3 goat isolates were serogroup D. All of 3 rabbit isolates were untypable. Overall 26 (34.6%) isolates were untypable. No strain was detected from serogroup B and E. Antimicrobial susceptibility test results of 50 P. multocida strains using disk diffusion test showed that all strains were susceptible to ceftiofur, enrofloxacin, florfenicol and trimethoprim-sulfamethoxazole. Six (12.0%) strains were resistant to spectinomycin, 6 (12.0%) to tetracycline, 3 (6.0%) to tilmicosin and 3 (6.0%) to erythromycin. Multiple resistance was detected in 7 (4 tetracycline+spectinomycin, 2 tilmicosin+erythromycin, 1 tetracycline + tilmicosin + erythromycin) of 11 resistant strains isolated from 10 cattle and 1 sheep.

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