Yıldıray BAŞBUĞAN, Ahmet Ufuk KÖMÜROĞLU, Deniz DİRİK, Uğur ÖZDEK, Pınar KOLUSARI, Volkan KOŞAL, Ömer Faruk KELEŞ

Ovaryum Torsiyonu Uygulanmış Ratlarda Chrisinin Koruyucu Etkisi

Bu çalışmadaki amacımız ovaryum torsiyon detorsiyon hasarına karşı chrysinin (CH) koruyucu etkisini değerlendirmektir. Otuz iki adet dişi wistar albino rat 4 gruba ayrıldı. Grup 1. Kontrol grubu, grup 2: TD grubu; grup 3: TD-CH grubu; grup 4: CH grubu. TD ve TD- CH gruplarına 3 saat torsiyon uygulandı ve 3 saatin sonunda detorsiyon yapıldı. TD-CH grubuna detorsiyondan yarım saat önce 50 mg/kg CH oral gavaj ile verildi. Daha sonra TD-CH ve CH gruplarına 14 gün boyunca 50 mg/kg CH oral gavaj ile verildi. Deneme sonunda ratlar sakrifiye edilerek kan örnekleri ve ovaryum dokusu alındı. Serum ve ovaryum dokusunda SOD ve GPx aktiviteleri, GSH ve 8-OHdG seviyeleri, serum örneklerinde ise 8-OHdG, IL-18, KIM-1, NGAL, Cys-C seviyeleri çalışıldı. TD grubunda hem serum hem de ovaryum dokusunda, GSH seviyeleri, GPx ve SOD aktiviteleri kontrol ve TD-CH gruplarından anlamlı olarak düşükken (P

Protective Eff ects of Chrysin in Rats with Ovarian Torsion

The objective of this study is to assess the protective eff ect of chrysin (CH) against ovarian torsion-detorsion injury. Thirty-two female albino ratswere divided into 4 groups as Control, Torsion/Detorsion (T/D), TD-CH, and CH. Ovarian torsion was done for 3 hours on TD and TD-CH groupsand then detorsion was performed. 50 mg/kg of CH was administered to the TD-CH group via oral gavage half an hour before the detorsion.Subsequently, 50 mg/kg of CH was administered via oral gavage to TD-CH and CH groups for 14 days. At the end of the experiment, bloodsamples were collected and ovarian tissue were taken. SOD and GPx activities and GSH and 8-OHdG levels were studied in serum and ovariantissue. Also, IL-18, KIM-1, NGAL, Cys-C levels were studied in serum samples. GSH levels and GPx, SOD activities in both serum and ovarian tissuewere significantly lower in TD group compared to the control and TD-CH groups (P

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