Zeynep BOZKAN, ALİ BELGE, MURAT SARIERLER, RECAİ TUNCA, RAHİME YAYGINGÜL, EMRAH İPEK

Comparison of the Efficacy of Amniotic Membrane Transplantation, Topical Water-Based Propolis Extract, Corticosteroid and Antibiotic Use in Diffrent Combinations on Subacute Corneal Alkali Burns in Rabbits

Investigation of the superiority of the usage of propolis extract, amniotic membrane transplantation, corticosteroid and antibiotic both separately and in diffrent combinations was aimed. A total of 40 (20 male, 20 female) New Zealand breed rabbits weighing 2.5-3.0 kg were used in the study. The rabbits were divided into 5 groups on day 7 of alkali burn injury. Amniotic membrane transplantation was applied to 3 groups and each one of these 3 groups had diffrent local medications as 1% water-based propolis extract (AMN+PRP), dexamethasone and tobramycin (AMN+AC) and only tobramycin (AMN+A). The other two groups, which were not performed amniotic membran transplantation, were either treated with propolis (PRP) or left as a control. The defect area results obtained from AMN+PRP group were lower than AMN+A group, and both AMN+PRP and control group at the day 9 and day 14, respectively (P≤0.05). In the measurements made after the second week, AMN+PRP group showed significantly better results in all weeks in terms of defect area (P≤0.05). Corneal thickness was found to be lower in all groups treated with amniotic membrane than in the other groups (P

Tavşanlarda Korneanın Subakut Alkali Yanıklarında Amniyotik Membran Transplantasyonu, Topikal Su Bazlı Propolis Ekstraktı, Kortikosteriod ve Antibiyotiğin Farklı Kombinasyonlarda Kullanımının Etkinliğinin Karşılaştırılması

Propolis ekstraktı, amniotik membran transplantasyonu, kortikosteroid ve antibiyotiğin ayrı ayrı ve farklı kombinasyonlarda kullanımının üstünlüğünün araştırılması amaçlandı. Çalışmada, 2.5-3.0 kg ağırlıkta toplam 40 Yeni Zelanda ırkı tavşan kullanıldı. Tavşanlar alkali yanık oluşturulmasının 7. gününde 5 gruba ayrıldı. Üç gruba amniyotik membran tranplantasyonu yapıldı ve bu gruplardan birine %1 su bazlı propolis ekstraktı (AMN+PRP), birine deksametazon ile tobramisin (AMN+AK), diğerine de sadece tobramisin (AMN+A) lokal olarak uygulandı. Diğer iki gruba amniyotik memran transplantasyonu yapılmadı, propolis ile tedavi edildi (PRP) veya kontrol olarak bırakıldı. AMN+PRP grubundan elde edilen defekt alanı sonuçları 9. günde AMN+A grubundan 14. günde hem AMN+A hem de kontrol grubundan daha düşüktü (P≤0.05). İkinci haftadan sonraki ölçümlerde AMN+PRP grubu defekt alanı bakımından tüm haftalarda daha iyi sonuç verdi (P≤0.05). Korneal kalınlık sonuçları ise amniotik membran ile tedavi gören tüm gruplarda düşüktü (P

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Ledbetter EC, Gilger BC: Diseases and Surgery of the Canine Cornea and Sclera, In, Gelatt KN, Gilger BC, Kern TJ (Eds): Veterinary Ophthalmology, 5 th ed., 976-1060, Wiley-Blackwell, USA, 2013.

Knollinger AM, McDonald JE, Carpenter NA, Crook EK: Use of equine amniotic membrane free-island grafts for treatment of a midstromal corneal ulcer and descemetocele in a snow leopard (Panthera uncia). J Am Vet Med Assoc, 253, 1623-1629, 2018. DOI: 10.2460/javma.253.12.1623

Barachetti L, Giudice C, Mortellaro CM: Amniotic membrane transplantation for the treatment of feline corneal sequestrum: Pilot study. Vet Ophthalmol, 13, 326-330, 2010. DOI: 10.1111/j.1463-5224.2010.00821.x

Chow DWY, Westermeyer HD: Retrospective evaluation of corneal reconstruction using ACell Vet™ alone in dogs and cats: 82 cases. Vet Ophthalmol, 19, 357-366, 2016. DOI: 10.1111/vop.12294

Jirsova K, Jones GLA: Amniotic membrane in ophthalmology: Properties, preparation, storage and indications for grafting-a review. Cell Tissue Bank, 18, 193-204, 2017. DOI: 10.1007/s10561-017-9618-5

Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian AM: Properties of the amniotic membrane for potential use in tissue engineering. Eur Cell Mater, 15, 88-99, 2008.

Kim JS, Kim JC, Na BK, Jeong JM, Song CY: Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acute corneal alkali burn. Exp Eye Res,70, 329-337, 2000. DOI: 10.1006/exer.1999.0794

Westekemper H, Figueiredo FC, Siah WF, Wagner N, Steuhl KP, Meller D: Clinical outcomes of amniotic membrane transplantation in the management of acute ocular chemical injury. Br J Ophthalmol, 101, 103-107, 2017. DOI: 10.1136/bjophthalmol-2015-308037

Bouchard CS, John T: Amniotic membrane transplantation in the management of severe ocular surface disease: Indications and outcomes. Ocul Surf, 2, 201-211, 2004. DOI: 10.1016/S1542-0124(12)70062-9

Deihim T, Yazdanpanah G, Niknejad H: Diffrent light transmittance of placental and reflcted regions of human amniotic membrane that could be crucial for corneal tissue engineering. Cornea, 35, 997-1003, 2016. DOI: 10.1097/ICO.0000000000000867

Cavendish RL, de Souza Santos J, Neto RB, Paixão AO, Oliveira JV, de Araujo ED, Silvia AAB, Thomazzi SM, Cardoso JC, Gomes, MZ: Antinociceptive and anti-inflmmatory effcts of Brazilian red propolis extract and formononetin in rodents. J Ethnopharmacol, 173, 127-133, 2015. DOI: 10.1016/j.jep.2015.07.022

Castaldo S, Capasso F: Propolis, an old remedy used in modern medicine. Fitoterapia, 73 (Suppl. 1): S1-S6, 2002. DOI: 10.1016/S0367- 326X(02)00185-5

Baki ME, Özcan M, Kerimoğlu G: Oral propolis treatment decelerates experimentally induced osteoarthritis in rats. J Clin Exp Med, 34, 191-194, 2017.

Öztürk F, Kurt E, Cerçi M, Emiroglu L, İnan Ü, Türker M, Ilker, S: The effct of propolis extract in experimental chemical corneal injury. Ophthalmic Res, 32, 13-18, 2000. DOI: 10.1159/000055581

Sá-Nunes A, Faccicoli LH, Sforcin JM: Propolis: Lymphocyte proliferation and IFN-γ production. J Ethnopharmacol, 87, 93-97, 2003. DOI: 10.1016/S0378-8741(03)00121-1

Kumazawa S, Hamasaka T, Nakayama T: Antioxidant activity of propolis of various geographic origins. Food Chem, 84, 329-339, 2004. DOI: 10.1016/S0308-8146(03)00216-4

Keshavarz M, Mostafaie A, Mansouri K, Shakiba Y, Motlagh HRM: Inhibition of corneal neovascularization with propolis extract. Arch Med Res, 40, 59-61, 2009. DOI: 10.1016/j.arcmed.2008.10.004

Lee HS, Lee JH, Kim CE, Yang JW: Anti-neovascular effect of chondrocyte-derived extracellular matrix on corneal alkaline burns in rabbits. Graefes Arch Clin Exp Ophthalmol, 252, 951-961, 2014. DOI: 10.1007/s00417-014-2633-3

Hansen PA, Guandalini A: A retrospective study of thirty cases of frozen lamellar corneal graft in dogs and cats. Vet Ophthalmol, 2, 233-241, 1999. DOI: 10.1046/j.1463-5224.1999.00084.x

Kim JC, Tseng SCG: The effects on inhibition of corneal neovascularization after human amniotic membrane transplantation in severely damaged rabbit corneas. Korean J Ophthalmol 9, 32-46, 1995.

Rootman DB, Trope GE, Rootman DS: Glaucoma aqueous drainage device erosion repair with buccal mucous membrane grafts. J Glaucoma, 18, 618-622, 2009. DOI: 10.1097/IJG.0b013e318193c472

Ziai S, Rootman DS, Slomovic AR, Chan CC: Oral buccal mucous membrane allograft with a corneal lamellar graft for the repair of Boston type 1 keratoprosthesis stromal melts. Cornea, 32, 1516-1519, 2013. DOI: 10.1097/ICO.0b013e3182a480f5

Kim JH, Chun YS, Lee SH, Mun SK, Jung HS, Lee SH, Son Y, Kim JC: Ocular surface reconstruction with autologous nasal mucosa in cicatricial ocular surface disease. Am J Ophthalmol, 149, 45-53, 2010. DOI: 10.1016/j. ajo.2009.07.030

Andrade AL, Laus JL, Figueiredo F, Batista CM: The use of preserved equine renal capsule to repair lamellar corneal lesions in normal dogs. Vet Ophthalmol, 2, 79-82, 1999. DOI: 10.1046/j.1463-5224.1999.00052.x

Liu J, Sheha H, Fu Y, Liang L, Tseng SC: Update on amniotic membrane transplantation. Expert Rev Ophthalmol, 5, 645-661, 2010. DOI: 10.1586/eop.10.63

Küçükerdönmez C, Akova YA, Altinors DD: Vascularization is more delayed in amniotic membrane graft than conjunctival autograft after pterygium excision. Am J Ophthalmol, 143, 245-249, 2007. DOI: 10.1016/j. ajo.2006.10.032

Wichayacoop T, Briksawan P, Tuntivanich P, Yibchok‐anun S: Anti-inflmmatory effcts of topical supernatant from human amniotic membrane cell culture on canine deep corneal ulcer after human amniotic membrane transplantation. Vet Ophthalmol, 12, 28-35, 2009. DOI: 10.1111/j.1463-5224.2009.00670.x

Lassaline ME, Brooks DE, Ollivier FJ, Komaromy AM, Kallberg ME, Gelatt KN: Equine amniotic membrane transplantation for corneal ulceration and keratomalacia in three horses. Vet Ophthalmol, 8, 311-317, 2005. DOI: 10.1111/j.1463-5224.2005.00405.x

Choi US, Labelle P, Kim S, Kim J, Cha J, Lee KC, Lee HB, Kim NS, Kim MS: Successful treatment of an unusually large corneal epithelial inclusion cyst using equine amniotic membrane in a dog. Vet Ophthalmol, 13, 122-125, 2010. DOI: 10.1111/j.1463-5224.2010.00765.x

Park JH, Jeoung JW, Wee WR, Lee JH, Kim MK, Lee JL: Clinical efficacy of amniotic membrane transplantation in the treatment of various ocular surface diseases. Cont Lens Anterior Eye, 31, 73-80, 2008. DOI: 10.1016/j.clae.2007.11.004

Takaoka M, Nakamura T, Sugai H, Bentley AJ, Nakajima N, Fullwood NJ, Yokoi N, Hyon SH, Kinoshita S: Sutureless amniotic membrane transplantation for ocular surface reconstruction with a chemically defined bioadhesive. Biomaterials, 29, 2923-2931, 2008. DOI: 10.1016/j.biomaterials.2008.03.027

Westekemper H, Figueiredo FC, Siah WF, Wagner N, Steuhl KP, Meller D: Clinical outcomes of amniotic membrane transplantation in the management of acute ocular chemical injury. Br J Ophthalmol, 101, 103-107, 2017. DOI: 10.1136/bjophthalmol-2015-308037

Subasi S, Altintas O, Yardimoglu M, Yazir Y, Karaman S, Rencber SF, Kavram K: Comparison of collagen cross-linking and amniotic membrane transplantation in an experimental alkali burn rabbit model. Cornea, 36, 1106-1115, 2017. DOI: 10.1097/ICO.0000000000001276

Eslani M, Baradaran-Rafii A, Cheung AY, Kurji KH, Hasani H, Djalilian AR, Holland EJ: Amniotic membrane transplantation in acute severe ocular chemical ınjury: A randomized clinical trial. Am J Ophthalmol, 199, 209-215, 2019 DOI: 10.1016/j.ajo.2018.11.001

Gheorghe A, Pop M, Burcea M, Serban M: New clinical application of amniotic membrane transplant for ocular surface disease. J Med Life, 9, 177-179, 2016.

Sharma N, Singh D, Maharana PK, Kriplani A, Velpandian T, Pandey RM, Vajpayee RB: Comparison of amniotic membrane transplantation and umbilical cord serum in acute ocular chemical burns: A randomized controlled trial. Am J Ophthalmol, 168, 157-163, 2016. DOI: 10.1016/j. ajo.2016.05.010

Glass LRD, Freitag SK: Orbital inflmmation: Corticosteroids first. Surv Ophthalmol, 61, 670-673, 2016. DOI: 10.1016/j.survophthal.2016.01.005

Chen HJ, Pires RTF, Tseng SCG: Amniotic membrane transplantation for severe neurotrophic corneal ulcers. Br J Ophthalmol, 84, 826-833, 2000. DOI: 10.1136/bjo.84.8.826

Baradaran-Rafii A, Eslani M, Haq Z, Shirzadeh E, Huvard MJ, Djalilian AR: Current and upcoming therapies for ocular surface chemical injuries. Ocul Surf, 15, 48-64, 2017. DOI: 10.1016/j.jtos.2016.09.002

Hepşen IF, Er H, Cekiç O: Topically applied water extract of propolis to suppress corneal neovascularization in rabbits. Ophthalmic Res, 31, 426-431, 1999. DOI: 10.1159/000055567

Totan Y, Aydin E, Cekiç O, Cihan Daglioglu M, Borazan M, Daglioglu K, Gültek A: Effct of caffic acid phenethyl ester on corneal neovascularization in rats. Curr Eye Res, 23, 291-297, 2001. DOI: 10.1076/ ceyr.23.4.291.5453

Clare G, Suleman H, Bunce D, Dua H: Amniotic membrane transplantation for acute ocular burns. Cochrane Database Syst Rev, 9, 1-41, 2012.

Yang LL, Zhou QJ, Wang Y, Gao Y, Wang YQ: Comparison of the therapeutic effects of extracts from Spirulina platensis and amniotic membrane on inflmmation-associated corneal neovascularization. Int J Ophthalmol, 5, 32-37, 2012.

Hopkinson A, Mclntosh RS, Tighe PJ, James DK, Dua HS: Amniotic membrane for ocular surface reconstruction: Donor variations and the effct of handling on TFG-β content. Invest Ophthalmol Vis Sci, 47, 4316- 4322, 2006. DOI: 10.1167/iovs.05-1415

Tamhane A, Vajpayee BB, Biswas NR, Pandey RM, Sharma N, Titiyal JS, Tandon R: Evaluation of amniotic membrane transplantation as an adjunct to medical therapy as compared with medical therapy alone in acute ocular burns. Ophthalmology, 112, 1963-1969, 2005. DOI: 10.1016/j. ophtha.2005.05.022

Lee HS, Lee JH, Kim CE, Yang JW: Anti-neovascular effect of chondrocyte-derived extracellular matrix on corneal alkaline burns in rabbits. Graefes Arch Clin Exp Ophthalmol, 252, 951-961, 2014. DOI: 10.1007/s00417-014-2633-3

Zheng J, Wei R, Zhang J, Wang Z, Zhu T, Ruan H, Song J: The efficacy of conjunctiva coverage in combination with amnion liquid supernatant eye drop on deep layer corneal ulcer in canine caused by alkali burn combined with mechanical injury. Kafkas Univ Vet Fak Derg, 25 (3): 365-372, 2019. DOI: 10.9775/kvfd.2018.21007

Pessolato AGT, dos S antos Martins D, Ambrósio CE, Mançanares CAF, de Carvalho AF: Propolis and amnion reepithelialise seconddegree burns in rats. Burns, 37, 1192-1201, 2011. DOI: 10.1016/j.burns. 2011.05.016