Emrah ŞİMŞEK, Arif ÇİLOĞLU, ALPARSLAN YILDIRIM, Gokmen Zafer PEKMEZCI

Identification and Molecular Characterization of Hysterothylacium (Nematoda: Raphidascarididae) Larvae in Bogue (Boops boops L.) from the Aegean Sea, Turkey

Hysterothylacium species are the most prevalent ascaridoid nematode reported from various freshwater, estuarial and marine fish species. However, there are very few studies about molecular identification and characterization of Hysterothylacium species in marine fish species from Turkish waters. The aim of the present study was to molecular identification and characterization of Hysterothylacium larvae from Boops boops caught off Aegean Sea, Turkey. Hysterothylacium larvae were found in 10 of 109 B. boops. The prevalence of Hysterothylacium spp. larvae was 9.2%. A total of 10 larvae of Hysterothylacium spp. were collected from all infected fish. Hysterothylacium larvae were genetically identified as H. aduncum, H. fabri and H. reliquens from Turkish waters by using sequence analyses of rDNA ITS regions in B. boops for the first time. In addition, B. boops was reported as a new host for H. reliquens. Moreover, H. reliquens was characterized for the first time by sequencing of the ITS regions from the Turkish waters with the present study.

Türkiye’nin Ege Denizi’nden Yakalanan Kupes (Boops boops) Balıklarında Hysterothylacium Larvalarının İdentifikasyonu ve Moleküler Karakterizasyonu

Hysterothylacium türleri çeşitli tatlı, acı su ve deniz balığı türlerinden bildirilen en yaygın ascaridoid nematodlardır. Ancak Türkiye sularındaki deniz balıklarında Hysterothylacium türlerinin moleküler tanımlanması ve karakterizasyonu konusunda çok az çalışma bulunmaktadır. Bu çalışmada Türkiye’nin Ege Denizi’nden yakalanan Boops boops’larda Hysterothylacium larvalarının moleküler tanımlanması ve karakterizasyonlarının belirlenmesi amaçlanmıştır. Hysterothylacium larvaları 109 B. boops’un 10’unda tespit edilmiş olup enfeksiyon oranı %9.2 olarak belirlenmiştir. Tüm enfekte balıklardan toplamda 10 adet Hysterothylacium larvası toplanmıştır. Türkiye sularından B. boops’larda ilk defa Hysterothylacium larvalarından rDNA ITS gen bölgesinin dizi analizleri ile H. aduncum, H. fabri ve H. reliquens türleri genetik olarak tanımlanmıştır. Ek olarak B. boops türü H. reliquens için yeni bir konak olarak rapor edilmiştir. Ayrıca, H. reliquens ITS gen bölgesinin sekans analizi ile Türkiye sularından ilk kez karakterize edilmiştir.

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