NADİDE NABİL KAMİLOĞLU, Ebru BEYTUT, ALKAN KAMİLOĞLU

Changes in lipid peroxidation and antioxidant environment of spinal fluid with the use of bupivacaine for spinal anesthesia

Bu çalışmada, bupivacain ile yapılan spinal anestezinin antioksidan savunma sistemi ve lipid peroksidasyonunun bir indikatörü olan tiyobarbiturik asit substratları (TBARS) düzeyleri, üzerine etkisini belirlemek amaçlanmıştır. Bunun için 15 klinik olarak sağlıklı 3-4 yaşlarında ve ortalama 56±5 kg ağırlığında Tuj koçları kullanılmıştır. Spinal anestezi oluşturmak amacıyla 8 ml %0.5’lik bupivacain kullanıldı. Bupivacain ile spinal anestezi yapılmadan önce (0. dakika) ve anesteziden 5, 30 ve 60 dakika sonra subarachnoid (intrathecal) bölgeden alınan spinal sıvı numuneleri thiobarbiturik asit substans (TBARS), E vitamini ve glutatyon (GSH) düzeyleri ile glutatyon peroxidaz (GSHPx, EC. 1.11.1.9) aktivitelerini belirlemek için kullanıldı. Bu çalışmada, TBARS düzeylerinin anesteziden 5 dak. sonra istatistiksel olarak artmaya başladığı (P

Anahtar Kelimeler:

glutatyon peroksidaz, lipit peroksidasyonu, farmakodinamik, koç, koyun, omurilik, E vitamini, anestetikler, anestezi, antioksidanlar, vücut sıvıları, serbest radikaller, glutatyon

Bupivacain ile yapılan spinal anestezi ile spinal sıvı lipid peroksidasyon düzeyi ve antioksidan sisteminde meydana gelen değişiklikler

The objective of this study was to determine if bupivacaine has any effects on the antioxidant defence system and in lipidperoxidation as thiobarbituric acid substance (TBARS) of spinal fluid. The experiment was carried out on 15 male clinicallyhealthy, 3-4 years old, male Tuj ram weighing an average 56±5 kg. Eight ml of 0.5% bupivacaine was used for the induction ofspinal anesthesia (SA). To measure the levels of lipid peroxidation (LPO), glutathione (GSH), activity of glutathione peroxidase (GSHPx; EC: 1.11.1.9) and the levels of vitamine E in spinal fluid, samples were taken from the subarachnoid (intrathecal) spacebefore anaesthesia (0. min) and at 5, 15, and 60 min. after anaesthesia. The levels of TBARS slightly increased at 5 min. afterspinal injection of bupivacaine and this increase continued trougout anesthesia (P<0.001). Vitamin E levels were decreased at5 min. after anesthesia (P<0.001) and this level recovered to control levels at 30 min. after bupivacaine injection. The activity of GSHPx and the levels of GSH in spinal fluid increased continually 5 min. after administration of bupivacain (P<0.001). Inconclusion, we found that while spinally injection of bupivacaine increased free radical levels in spinal fluid may be supportedantioxidant environment of spinal fluid during anesthesia.

Keywords:

glutathione peroxidase, lipid peroxidation, pharmacodynamics, rams, sheep, spinal cord, vitamin E, anaesthetics, anaesthesia, antioxidants, body fluids, free radicals, glutathione,

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