Effects of laminar flow ultraviolet sterilization Protocol on Cryptosporidium parvum oocysts and determination of these effects with vital dyes

Cryptosporidiosis dünya genelinde yaygın olarak görülen, zoonotik karakterde bir hastalıktır. Önemli ekonomik kayıplara neden olabilen hastalıkla mücadelede korunma ve kontrol büyük önem arz etmektedir. Bu noktada, hastalığın enfeksiyöz formu olan oocystlerinin inaktivasyonunu sağlamak amacıyla UV (ultraviolet) kullanılabilmektedir. Bu çalışmada, rutin hücre kültürü laboratuvarları steril kabinlerinde kullanılan UV sterilizasyon işleminin Cryptosporidium parvum oocystleri üzerindeki etkisinin ve UV uygulamasına bağlı olarak oocystlerde meydana gelen değişikliklerin belirlenmesi konusunda vital boyaların (DAPI, PI) etkinliklerinin belirlenmesi amaçlanmıştır.

Anahtar Kelimeler:

ışınlama, zar geçirgenliği, ookist, ultraviyole ışıması, Cryptosporidium parvum, hücre zarı, boyalar, inaktivasyon

Cryptosporidium parvum oocystleri üzerine laminar flow ultraviole sterilizasyon protokolünün etkisi ve söz konusu etkinin vital boyalarla belirlenmesi

Cryptosporidiosis is a globally widespread protozoan disease with zoonotic character. Protection and control bear greatimportance for combating the disease that may cause serious economic losses. At this point, UV (ultraviolet) may be used toinactivate the oocysts those are infectious form of the disease. In this study, determination of the effect of UV sterilizationprocess used in routine cell culture laboratory sterile cabins (laminar fl ow) on Cryptosporidium parvum oocysts and the efficiencyof vital dyes (DAPI, PI) in the demonstration of the changes made by UV applications in question on oocysts is aimed.

Keywords:

irradiation, membrane permeability, oocysts, ultraviolet radiation, Cryptosporidium parvum, cell membranes, dyes, inactivation,

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