Öznur ASLAN, İLKNUR KARACA BEKDİK, Esma Gamze İLGAR

Bir kedide Mycoplasma haemofelis enfeksiyonu ve etkenin taramalı elektron mikroskopi ile görüntülenmesi

Bu olgu sunumunda, bir kedide Mycoplasma haemofelis enfeksiyonunun ve elektron mikroskopik görüntülenmesinin değerlendirilmesi amaçlandı. Olgunun materyalini iştahsızlık, halsizlik, dehidrasyon şikayeti olan, 3 yaşlı, erkek, sarman kedi oluşturdu. Kedinin yapılan klinik muayenesinde şiddetli dehidrasyon, apati, taşipne, kalpte üfürüm, taşikardi ve beden ısısının 38.2°C olduğu belirlendi. Kan örneklerinin sitolojik değerlendirilmesi ve polimeraz zincir reaksiyonu (PZR) ile Mycoplasma haemofelis tanısı kondu ve taramalı elektron mikroskobi ile görüntülendi. Sağaltım amacıyla oral yolla 14 gün süreyle 10 mg/kg/gün dozunda doksisiklin (Tetradox kapsül, Fako®) kullanıldı. Sağaltım sonucunda kedinin klinik durumunun düzeldiği, kan frotisinde etkenlerin sayısının azaldığı, kan hemogram ve serum biyokimyasal değerlerinin normale döndüğü belirlendi. Yapılan literatür araştırmasına göre ülkemizde Mycoplasma haemofelis ile doğal enfekte bir kedide etkenin taramalı elektron mikroskopi (TEM) yöntemiyle görüntülenmesi ilk kez gerçekleştirildi.

Mycoplasma haemofelis infection and imaging of Mycoplasma haemofelis using scanning electron microscopy in a cat

In this case report was aimed to the evaluation of Mycoplasma haemofelis infection and electron microscopic imaging in a cat. The material of present case was included a cat, three years old, male, referred to a history of anorexia, lethargy and dehydration. Clinically were defined dehydration, apathy, tachypnea, 38.2 º C body temperature, murmur and tachycardia at the auscultation of heart. Mycoplasma haemofelis was detected at the peripheral smear and using PCR assay, and viewed with scanning electron microscopy. The patient was treated with doxycycline (Tetradox kapsül, Fako®) at a dose of 10 mg/kg/day orally for 14 days. The clinical condition of the cat was improved after treatment, which reduced the number of Mycoplasma haemofelis in blood smears, hematological and serum biochemical values were determined to be normal. To the best of authors knowledge, this case reports the first imaging of scanning electron microscopy of Mycoplasma haemofelis in cats that are naturally infected in Turkey.

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1. Barker E, Tasker S: Haemoplasmas: Lessons learnt from cats. N Z Vet J, 61 (4): 184-192, 2013.
2. Tasker S: Haemotropic mycoplasmas: Whats their real significance in cats? J Feline Med Surg, 12 (5): 369-381, 2010.
3. Torkan S, Aldavood SJ, Rafie SM, Hejazi H, Shirani D, Momtaz H: Prevalence and risk factor analysis of Haemobartonella felis in cats using direct blood smear and PCR assay. Comp Clin Path , 22 (6): 1103-1109, 2013.
4. Sykes JE: Feline hemotropic mycoplasmas. J Vet Emerg Crit Care (San Antonio), 20 (1): 62-69, 2010.
5. Willi B, Tasker S, Boretti FS, Doherr MG, Cattori V, Meli ML, Lobetti RG, Malik R, Reusch CE, Lutz H, Hofmann-Lehmann R: Phylogenetic analysis of Candidatus Mycoplasma turicensis isolates from pet cats in the United Kingdom, Australia, and South Africa, with analysis of risk factors for infection. J Clin Microbiol, 44 (12): 4430-4435, 2006.
6. Tasker S, Caney SM, Day MJ, Dean RS, Helps CR, Knowles TG, Lait PJ, Pinches MD, Grufydd-Jones TJ: Efect of chronic FIV infection, and efficacy of marbofoxacin treatment, on Mycoplasma haemofelis infection. Vet Microbiol, 117 (2-4): 169-179, 2006.
7. Tasker S, Helps CR, Day MJ, Grufydd-Jones TJ, Harbour DA: Use of real-time PCR to detect and quantify Mycoplasma haemofelis and Candidatus Mycoplasma haemominutum DNA. J Clin Microbiol, 41 (1): 439- 441, 2003.
8. Carney HC, England JJ: Feline hemobartonellosis. Vet Clin North Am: Small Anim Pract, 23 (1): 79-90, 1993.
9. Harvey JW, Gaskin JM: Feline haemobartonellosis: Attempts to induce relapses of clinical disease in chronically infected cats. J Am Anim Hosp Assoc , 14 (4): 453-456, 1978.
10. Tasker S, Lappin MR: Haemobartonella felis: Recent developments in diagnosis and treatment. J Feline Med Surg, 4 (1): 3-11, 2002.
11. Willi B, Museux K, Novacco M, Schraner EM, Wild P, Groebel K, Ziegler U, Wolf-Jäckel GA, Kessler Y, Geret C, Tasker S, Lutz H, Hofmann-Lehmann R: First morphological characterization of Candidatus Mycoplasma turicensis using electron microscopy. Vet Microbiol, 149 (3- 4): 367-373, 2011.
12. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manuel. 2 nd ed., Vol. 3, 914-923, Cold Spring Harbor Lab. Press, New York, 1989.
13. Devrim AK, Kaya N: Polimeraz zincir reaksiyonu. Kafkas Univ Vet Fak Derg, 10 (2): 209-214, 2004.
14. Jensen WA, Lappin MR, Kamkar S, Reagan WJ: Use of a polymerase chain reaction assay to detect and differentiate two strains of Haemobartonella felis in naturally infected cats. Am J Vet Res. 62 (4): 604- 608, 2001.
15. Grindem CB, Corbett WT, Tomkins MT: Risk factors for Haemobartonella felis infection in cats. J Am Vet Med Assoc, 196 (1): 96-99, 1990.
16. Atalay T: Kayseri yöresindeki kedilerde feline infeksiyöz aneminin varlığının belirlenmesi. Yüksek Lisans Tezi, Erciyes Üniv. Sağlık Bil. Enst., 2013.
17. Flint JC, Roepke MH, Jensen R: Feline infections anemia. II. Experimental cases. Am J Vet Res, 20, 33-40, 1959.
18. Korman RM, Ceron JJ, Knowles TG, Barker EN, Eckersall PD, Tasker S: Acute phase response to Mycoplasma haemofelis and Canditatus mycoplasma haemominutum infection in FIV-infected and non-FIV- infected cats. Vet J, 193 (2): 433-438, 2012.
19. Messick JB: New perspectives about hemotropic mycoplasma (formerly, Haemobartonella and Eperythrozoon species) infections in dogs and cats. Vet Clin North Am: Small Anim Pract, 33 (6): 1453-1465, 2003.
20. Aslan Ö, İça A, Çam Y, Kibar M: Kayseride bir kedide haemo- bartonellozis olgusu. Erciyes Üniv Vet Fak Derg , 7 (2): 131-135, 2010.
21. Tasker S, Peters IR, Papasouliotis K, Cue SM, Willi B, Hofmann- Lehmann R, Gruffydd-Jones TJ, Knowles TG, Day MJ, Helps CR: Description of outcomes of experimental infection with feline haemoplasmas: Copy numbers, haematology, Coombs testing and blood glucose concentrations. Vet Microbiol, 139 (3-4): 323-332, 2009.
22. Ural K: Hemobartonellozisli kedilerde klinik, hematolojik bulgular, FIV/FeLV enfeksiyonları ile sağaltımda enrofloksasin uygulamaları. Doktora Tezi, Ankara Üniv. Sağlık Bil. Enst., 2006.