Wei YANG, Chuang XU, Cheng XIA, Yuanyuan CHEN, Jiasan ZHENG, Xinwei LI, Guowen LIU, Xiaobing LI

Negatif Enerji Dengesiyle Oluşturulmuş Koyun Yağlı Karaciğerinin Ultrasonografik Değerlendirmesi

Yağlı karaciğer hastalığı insanlarda ve benzer olarak hayvanlarda yaygın bir karaciğer hastalığı olup, makrofauna yağlı karaciğer hastalığının ultrasonografi (US)-temelli diagnostik araştırmaları için ideal bir modeldir. Bu çalışmada, laktasyondaki ince kuyruklu koyun ırkında negatif enerji dengesi ile yağlı karaciğer hastalığı modeli beslemenin kısıtlaması yoluyla oluşturulmuştur. Ultrasonografik görüntüler karaciğer trigliserid konsantrasyonu (yaş karaciğer ağırlığının trigliserid ağırlığına oranı) baz alınarak yağ sınıflarına ayrılmıştır; sağlıklı karaciğer, =2% ile 5% arası; orta derecede HS, >=5% ile 10% arası ve şiddetli HS, >=10%. HS şiddetine göre ultrasonografik görüntülerin özellikleri değerlendirildi. Sonuç olarak kısıtlı besleme ile hafif ve orta derecelerde HS tespit edildi. Sağlıklı, hafif, orta ve şiddetli HS'nin diagnostik ayırımı için 5 MHz'nin hassasiyeti sırasıyla %66.7, %60.0, %66.7 ve %82.5 olarak belirlendi. Gözlemciler arasında ortalama ? istatistik 0.667 ile 0.735 arasında değişim gösterdi. Sağlıklı, hafif, orta ve şiddetli HS'nin diagnostik ayırımı için 3.5 MHz'nin hassasiyeti sırasıyla %69.7, %60.5, %81.5 ve %75.4 olarak belirlendi. Gözlemciler arasında ortalama ? istatistik 0.575 ile 0.684 arasında değişim gösterdi. Orta ve şiddetli HS için 3.5 ve 5 MHz'lerin hassasiyetlikleri sırasıyla %97.4 ve %98.2'ye ulaştı. Özet olarak, 3.5 ve 5 MHz'lerde görüntüleme hafif vakalar haricinde HS'nin tanısı için tespit edilmiş olan US tanı standartlarında idealdir. Ayrıca, bu model görüntü dijital analiz gibi yeni metotlar ile birlikte yağlı karaciğerin US temelli tanısında ileriki araştırmalarda kullanılabilir.

Ultrasound Evaluation of Negative Energy Balance-Induced Fatty Liver in Sheep

Fatty liver disease is a common liver disease in humans and animals alike, and macrofauna are considered an ideal model of fatty liver disease for ultrasonography (US)-based diagnostic research. Here, a model of fatty liver disease in lactating thin-tail sheep induced by negative energy balance was developed by restricted feeding. Ultrasonographic images were divided into the following fat classes according to liver triglyceride concentrations (ratio of triglyceride weight to wet liver weight): healthy liver, <2%; mild hepatic steatosis (HS), >=2% to 5%; moderate HS, >=5% to 10%; and severe HS, >=10%. Characteristics of Ultrasonographic images according to HS severity were evaluated. As the results, mild to severe HS was detected by restricted feeding. The sensitivity of 5 MHz for diagnostic differentiation of healthy liver, and mild, moderate, and severe HS were 66.7%, 60.0%, 66.7%, and 82.5%, respectively, with a moderate ? statistic ranging from 0.667 to 0.735 among observers. The sensitivity of 3.5 MHz for diagnosis of healthy liver, and mild, moderate, and severe HS were 69.7%, 60.5%, 81.5%, and 75.4%, respectively, with moderate ? statistics ranging from 0.575 to 0.684 among observers. The sensitivities of 3.5 and 5 MHz for moderate to severe HS reached 97.4% and 98.2%, respectively. In summary, visualization at 3.5 and 5 MHz was ideal for diagnosis of HS except mild cases with established US diagnostic standards. Furthermore, with the established model, newer methods, such as image digitizing analysis, can be used for US-based diagnosis of fatty liver in further investigations.

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