YAKUP YILDIRIM, VOLKAN YILMAZ, ATİLA TANER KALAYCIOĞLU, SEVAL BİLGE DAĞALP, Ali Rıza Farajı MAJARASHIN, ÖZGÜR ÇELEBİ, DOĞAN AKÇA

An investigation of A possible involvement of BVDV, BHV-1 and BHV-4 infections in abortion of dairy cattle in Kars district of Turkey

Bu araştırmada, Kars yöresinde süt sığırı sürülerinde görülen abort vakalarında BVDV, BHV-1 ve BHV-4 enfeksiyonlarının olası etkileri serolojik olarak araştırıldı. Kan serumu örnekleri son yıllarda abort vakalarının sıklıkla görüldüğü gebeliğin ilk ve ikinci üç aylık dönemlerinde, atık yapmış 140 sığırdan toplandı. Kan serumu örnekleri BHV-1 ve BHV-4 spesifik antikorların varlığı ELISA, BVDV spesifik antikorların varlığı ise nötralizasyon testleri ile analiz edildi. ELISA tekniği ile örneklerin %61.4’ünde (86/140) BHV-1 ve %29.3 (41/140)’ünde BHV-4 spesifik antikorlar yönünden pozitiflik saptandı. Virus nötralizasyon testi ile de örneklerin %52.9’unun (74/140) BVDV’üne spesifik nötralizan antikor taşıdığı belirlendi. Örneklerin %11.4’ünün ise üç enfeksiyona da spesifik antikorlara sahip olduğu tespit edildi. Sonuçlar değerlendirildiğinde, Kars yöresinde meydana gelen abort vakalarından diğer enfeksiyöz ajanların yanı sıra BHV-4, BHV-1 ve BVDV enfeksiyonlarının da sorumlu olabileceği anlaşılmaktadır. Ayrıca örneklenen sürülerde yüksek BVDV seroprevalansı’nın varlığı, sürü içerisinde persiste enfekte hayvanların varlığını akla getirmektedir. Bu çalışma’nın sonuçları işığında viral enfeksiyonların neden olabileceği abort vakalarının kontrolü için aşılama çalışmalarının, gebelik döneminden önce yapılmasının faydalı olacağı kanaatine varılmıştır.

Kars yöresindeki süt sığırlarında görülen abort vakalarında BVDV, BHV-1 ve BHV-4 enfeksiyonlarının olası etkilerinin araştırılması

In this study, the possible effects of BHV-1, BHV-4 and BVDV involving abortions in the selected dairy cattle herds in Kars province of Turkey were serologically investigated. Serum samples obtained from 140 aborted cattle where frequent abortion cases frequently occurred during first and second trimesters of gestation last a few years were analyzed for the presence of specific antibodies for BHV-1 and BHV-4 using ELISA and BVDV using neutralization test, respectively. The results showed that 61.4% (86/140) and 29.3% (41/140) of samples were positive for BHV-1 and BHV-4 specific antibodies by ELISA respectively and 74 (52.9%) of tested samples had neutralizing antibodies to BVDV in virus neutralization test. Furthermore, the results also showed that 11.4% of the samples (16/140) had antibody against all of BHV-4, BHV-1 and BVDV. From our results, BHV-1, BHV-4 and BVDV may be responsible for causing abortions and BVDV seroprevalece in tested animals may be indicative for the presence of the persistently infected animals in Kars pasture. The control of abortions caused by viral infections vaccinations should be practiced before the gestation time of cattle.

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