ABCG2 gene polymorphism in Holstein cows of Turkey

ABCG2 proteini ya da Breast Cancer Resistant Protein (Bcrp) meme bezi alveoler epitel hücre membranında lokalize olan en önemli aktif transport proteinlerinden biridir ve ilaçların kandan süte transferinden sorumludur. ABCG2 proteini aynı adlı gen (ABCG2 ya da Bcrp) tarafından kodlanır. Bu transport proteinleri veteriner hekimlikte oldukça önemlidir. İsrail kökenli Holştayn ırkı ineklerde, Bcrp‘ni kodlayan gende (ABCG2), aminoasit değişimine (Y581S) neden olan tek nükleotid polimorfizmi, hayvanların süt verimini ve süt özelliklerini etkilemektedir. ABCG2A alleline sahip İsrail Holştayn ırkı hayvanlarda süt veriminin düştüğü ancak sütün yağ ve protein oranının arttığı bilinmektedir. Bu çalışmada Türkiye kökenli Holştayn ırkı ineklerde ABCG2 geninde sözü edilen allel sıklığı araştırılmış ve bu amaçla Trakya bölgesinde 207 adetlik sınırlı bir Holştayn popülasyonu genotiplenmiştir. İncelenen hayvanların tümünde A alleli predominant bulunmuştur (0.9807). Bu ineklerden 8’inde C alleli (0.0193) saptanırken incelenen hiçbir hayvanda CC homozigotluk bulunamamıştır.

Anahtar Kelimeler:

Alacalı Sığır, Türkiye, alleller, sığır, inek, süt sığırı, süt ineği, gen frekansı, genler, genetik polimorfizm, meme bezleri, süt bileşimi, süt verimi

Türkiye Holstein ırkı ineklerinde ABCG2 gen polimofizmi

ABCG2 protein or Breast Cancer Resistant Protein (Bcrp), located in membrane of mammary glands alveolar epithelial cells, is one of the most important active transport proteins and responsible for passing of drugs from blood to milk. It is encoded by ABCG2 (Bcrp). These transport proteins are very important for veterinary medicine. Single nucleotide polymorphism, which is caused by changing amino acid (Y581S), in ABCG2 gene that encodes Bcrp, affect milk yield and milk properties in Israeli Holstein. It is known that fat and protein proportion in milk were increased, but milk yield was decreased in Israeli Holstein which have ABCG2A allele. In this study, allele frequency of ABCG2 was investigated in Holstein cows of Turkey and for this purpose 207 Holstein cows from Trakya region were genotyped. A allele (0.9807) had been found dominant in all investigated animals. C allele (0.0193) had been determined at only 8 animals. On the other hand CC homozygous had not been found in investigated animals.

Keywords:

Holstein-Friesian, Turkey, alleles, cattle, cows, dairy cattle, dairy cows, gene frequency, genes, genetic polymorphism, mammary glands, milk composition, milk yield,

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